Comet Assay Tanks

Product Description

Gentaur Comet Assay Tanks are available in four slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantifation of DNA damage in cells. Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis DNA damage may be measured using Comet Assay scoring software.

Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells exposed to irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA lesions and repair processes.

Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a comet as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis.

Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using software.

Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is concentrated within and towards the tail.

Variants:

• CSL-COM10 - Comet Assay Tank for 10 Slides

• CSL-COM20 - Comet Assay Tank for 20 Slides

• CSL-COM40 - Comet Assay Tank for 40 Slides

• CSL-COM80 - Comet Assay Tank for 80 Slides

Clinical Electrophoresis

Cellulose acetate electrophoresis is an important technique in clinical diagnostics. Gentaur’s CellasGEL range is a complete system solution for research and clinical cellulose acetate electrophoresis applications. The CellasGEL range includes both equipment and consumables to assist in the research and diagnosis of specific disease states.

Cellas Electrophoresis System

 

Product Description

The ideal tank for standard membrane and gel cellulose acetate techniques, the Cellas electrophoresis system is designed and built to our high quality standard to address both routine clinical and research requirements. Two adjustable supports, which can be positioned anywhere within the tank, readily accommodate different lengths of dry cellulose acetate membrane to a maximum 20cm.

Variant:

• CSL-CELLAS - Horizontal Unit for Cellulose Acetate Electrophoresis

 

Applicators

 

Product Description

Unlike dry cellulose acetate strips that are restricted to low volume micro tests, CellasGEL’s greater thickness allows tests to be performed on semi-micro and macro scales using a wide range of specialist applicators. Consequently greater sample-volumes may be loaded as larger, but finer bands over a wider front. This reduces sample saturation and aids densitometric band quantitation, thereby improving resolution.

Variants:

• CSLAPPS22    - 1x 2-specimen semi-micro applicator 0.7μl / 7mm, 2.5x14cm

• CSLAPPS4SP - 1x 4-specimen semi-micro applicator 0.9μl / 9mm, 5.7x14cm

• CSLAPPS4       - 1x 4-specimen semi-micro applicator 1.2μl / 9mm, 5.7x14cm

• CSLAPPS6       - 1x 6-specimen semi-micro applicator 0.7μl / 7mm, 5.7x14cm

• CSLAPPM8     - 1x 8-sample micro applicator 0.3μl /5mm, 5.7 x 14cm

 

Densitometer

Product Description

A universal densitometer scanner with software capable of reading 144 tests per minute is available for band quantitation following post-electrophoretic staining.

In terms of flexibility, TurboScan puts all previous systems in the shade. It allows you to create as many individually generated scan masks as you wish. You can also select as many analysis methods as you wish. No other system offers so many options. In clinical work, for example, you can use TurboScan for analysis in the following applications: serum protein electrophoresis, lipo-protein electrophoresis, haemoglobin electrophoresis, Hb-A1c electrophoresis, urine electrophoresis, CSF electrophoresis, Bence-Jones, isoenzymes, immunofixation, isoelectric focusing, multifractional electrophoresis, blots and lots more. It does not matter if you carry out your methods on dry or wet cellulose acetate strips, on agarose and on other gels or you work with micro, semi-micro or macro application. TurboScan uses the latest digital image analysis technology.

Variations

• CSLDENS - TurboScan Software Densitometer (excludes computer and scanner)

• CSLSCAN - Flatbed scanner for TurboScan software

 

Bridge for Cellas Gel Strips

Product Description

A universal bridge supports each 2.5 x 14cm and 5.7 x 14cm CellasGEL during sample application by serving as a convenient loading template for the required applicator. Up to three universal bridges may be then placed into each Cellas tank for electrophoresis.

Variant:

• CSLBDG8.5S - Bridge 8.5 cm (standard size for CellasGEL kits) for CellasGEL strips 5.7×14 or 2.5x14cm

 

CellasKIT: Lipoproteins

 

Product Description

4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250mm); 3 strips / CSL-CELLAS performed on 3x bridges.

Kit content, sufficient for 100x semi-micro tests:

25 Cellogel strips & Tris- Hippurate buffer; Sudan Black stain & clearing solution; blotting paper, mylar film and 1 plastic box.

Variant:

• CSLKITLP100 - CellasKIT LIPOPROTEINS (100 semimicro tests) on CellasGEL (Accessories required: 1x CSLAPPS4, 3x CSLBDG8.5S)

• CSLKITLP100-ABS - Includes 1 x CSLKITLP100, 1 X CSLAPPS4, 3 X CSLBDG8.5S

 

CellasKIT: Haemoglobin

Product Description

4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250mm); 3 strips / CSL-CELLAS performed on 3x bridges.

Kit content, sufficient for 100x semi-micro tests:

25 CellasGEL strips & Tris-Glycine buffer; Ponceau S stain, destain & clearing solutions; blotting paper & mylar film.

Variant:

• CSLKITHG100- CellasKIT HEMOGLOBINS (100 semimicro tests) on CellasGEL (Accessories required: 1x CSLAPPS4, 3x CSLBDG8.5S)

• CSLKITHG100-ABS - Includes 1 x CSLKITHG100, 1 X CSLAPPS4, 3 X CSLBDG8.5S

 

CellasKIT: Serum Proteins (high resolution)

 

Product Description

 

6x semi-micro or 8x micro tests for each CellasGEL strip (5.7x14cm; 190mm); 3 strips / CSL-CELLAS performed on 3x bridges.

 

Kit content, sufficient for 150x semi-micro or 200x micro tests:

 

25 CellasGEL strips & Tris-Hippurate buffer; Coomassie stain, citric acid & clearing solutions; blotting paper & mylar film.

 

Variant

 

• CSLKITSP150200 - CellasKIT HIGH RESOLUTION OF SERUM PROTEINS,150 semimicro or 200 micro tests, on CellasGEL (Accessories required1x CSLAPPS6, 3x CSLBDG8.5S or 1x CSLAPPM8, 3x CSLBDG8.5S)

 

• CSLKITSPHR-ABS - Includes 1 x CSLKITSP150200, 1 X CSLAPPS4, 3 X CSLBDG8.5S

 

• CSLKITSPHR-ABM - Includes 1 x CSLKITSP150200, 1 X CSLAPPM8, 3 X CSLBDG8.5S

 

CellasKIT: Serum Proteins

 

Product Description

 

4x semi-micro or 8x micro tests for each CellasGEL strip (5.7x14cm; 200mm); 3 strips / CSL-CELLAS performed on 3x bridges.

 

Kit content, sufficient for 100x semi-micro or 200x micro tests:

 

25 CellasGEL strips & Tris-Hippurate buffer; Ponceau S stain, destain & clearing solutions; blotting paper & mylar film.

 

Variant:

 

• CSLKITSP100200 - CellasKIT SERUM PROTEINS,100 semimicro or 200 micro tests, on CellasGEL (Accessories required 1x CPPS4SP, 3x CSLBDG8.5S or 1x CSLAPPM8, 3x CSLBDG8.5S)

 

• CSLKITSP-ABS -Includes 1 x CSLKITSP100200, 1 X CSLAPPS4 & 3 X CSLBDG8.5S

 

• CSLKITSP-ABM - Includes 1 x CSLKITSP100200, 1 X CSLAPPM8, 3 X CSLBDG8.5S

 

 

RunVIEW

Real-time size fractionation and recovery of nucleic acids

RunVIEW is an innovative new system which can be used to maximise the efficiency of DNA recovery from EtBr and SYBR stained gels by minimising the number of steps involved in postelectrophoretic purification. RunVIEW consists of a MultiSUB™ MSCHOICE system with BluVIEW lid, containing an orange spectral emission filter within its viewing pane, and a base unit with integrated power supply and blue LED gel illuminator.

RunVIEW

 

Product Description

 

Using RunVIEW is simple; first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the MSCHOICE tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (loading tier). Replace the lid to connect the MSCHOICE tank to the integrated power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid viewing pane as the samples migrate in real-time to the second row of wells (extraction tier). Once the DNA bands of interest enter the tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.

 

Track DNA without harmful UV

 

Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning efficiency. RunVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.

 

Save on time-consuming gel elution techniques

 

Running DNA into a well containing buffer effectively purifies the DNA of agarose, eliminating the need for time-consuming gel excision and purification techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.

 

No expensive commercial gels

 

RunVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15×7, 15×10 or 15x15cm MSCHOICE gel trays, and therefore does not require expensive precast gels and accessories.

 

A self-contained system

 

The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.

 

Variations:

 

• CSL-RVMSCHOICE – RunVIEW system complete with 15 x 7cm gel tray, 1 set of casting dams and 8 double-sided combs

 

• CSL-RVMSCHOICE10 - RunVIEW system complete with 15 x 10cm gel tray, 1 set of casting dams and 8 double-sided combs

 

• CSL-RVMSCHOICE15 - RunVIEW system complete with 15 x 15cm gel tray, 1 set of casting dams and 8 double-sided combs

 

• CSL-RVMSCHOICETRIO - RunVIEW system complete with 15 x 7, 15 x 10 and 15 x 15cm gel trays, 3 sets of casting dams and 8 double-sided combs

 

• CSL-RVBSBVLID – RunVIEW base station & bluVIEW lid

 

• CSL-RVGELDOC – RunVIEW gel documentation hood with 10MP camera

 

runSafe

 

Product Description

 

runSafe – A new ready to use safe and sensitive alternative to ethidium bromide and other DNA stains!

 

runSafe is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of gels. Supplied in Cleaver Scientific’s 6X DNA Loading Buffer, runSafe is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. runSafe is a highly sensitive stain available for detecting doublestranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visual tracking of DNA migration during electrophoresis. It is ideal for the environment providing a safe, sensitive,
non-mutagenic and non-hazardous alternative to Ethidum Bromide

 

See your samples separate in real time

 

Simply mix, load and run – runSafe acts as both a loading dye and a DNA stain combined for added speed and convenience. Economic and Low environmental impact – No expensive waste management is required and complies with Clean Water Act standards. runSafe – Supplied in 6X Loading Buffer

 

• CSL-RUNSAFE: 1 ml/ vial

 

runDOC

 

Product Description

 

RunDOC is a portable, lightweight gel documentation system designed exclusively for use with RunVIEW – the innovative new system designed for
real-time size fractionation and recovery of nucleic acids. RunDOC comprises a darkroom hood and 12.1 megapixel digital camera to capture images of nucleic acid gels stained with EtBr, SYBR and runSAFE. The RunDOC darkroom hood is placed directly over the RVMSCHOICE gel tank located on the RunVIEW base unit, which includes an integrated power supply and blue LED gel illuminator. The blue LED illuminator provides the excitation source for the DNA gel located within the tank, and following excitation the light emitted is then visualized using either one of the bluVIEW lid options (supplied with RunVIEW and used with the RVMSCHOICE gel tank) or the RunDOC filter slide. RunDOC also benefits from a small footprint area, occupying minimal space within the laboratory.

 

Variants:

 

• CSL-RVGELDOC – RunVIEW gel documentation hood with camera and filter slides

 

• CSL-RVGELDOCSYS - CSL-RVGELDOC plus note book PC and 1D analysis software

 

• CSL-RVSTATION – RunSTATION complete with CSL-RVGELDOC & CSL-RVMSCHOICETRIO

 

• CSL-RVGDCOMPLETE - Complete System; RVMSCHOICETRIO & RVGELDOCSYS

MultiSUB Horizontal Gel Systems

Gentaur’s MultiSUB™ horizontal gel electrophoresis units have been designed by scientists for scientists. With its advanced features, each unit provides an easy to use, versatile and flexible system that can evolve and adapt with the changing needs of today’s laboratory researcher.

For prices click on the links

MultiSUB Mini

 

Product Description

The MultiSUB Mini is the smallest unit in the range, designed for low to medium numbers of samples. The small gel size maximises run economy but does not compromise versatility as two tray options are available,  7 x 7cm and 7 x 10cm,  and combs ranging from preparative up to 16 samples. Simply by altering the gel tray or comb, this compact unit is capable of resolving up to 64 different samples, prepping 1ml of sample or separating sample bands over a distance of 9cm.

Buffer saver blocks physically reduce the volume of a gel chamber and buffer requirements, therefore saving cost.

Variants:

• MSMINI7 – MultiSUB Mini, 7 x 7cm UV Tray, 2 x 8 sample combs, loading guides and dams

• MSMINI10 - MultiSUB Mini, 7 x 10cm UV Tray, 2 x 8 sample combs, loading guides and dams

• MSMINIDUO - MultiSUB Mini, 7 x 7cm & 7 x 10cm UV Tray, 2 x 8 sample combs, loading guides and dams

 

MultiSUB Midi

Product Description

With gel tray options of 10 x 7cm and 10 x 10cm, the MultiSUB Midi has been designed for routine horizontal gel electrophoresis. Extending the width of this unit allows more samples to be resolved per gel than the MultiSUB Mini, without a significant increase in buffer or gel volumes.

A maximum of 100 samples per gel can be resolved, making this unit ideal for those routinely checking medium numbers of samples over short to medium gel run lengths. Loading guides allow easy well identification and sample loading. Scoops are also available to allow safe transfer of gels.

Variants:

• MSMIDI7 – MultiSUB Midi, 10 x 7cm UV tray, 2 x 16 sample combs, loading guides and dams

• MSMIDI10 - MultiSUB Midi, 10 x 10cm UV tray, 2 x 16 sample combs, loading guides and dams

• MSMIDIDUO - MultiSUB Midi, 10 x 7cm & 10 x 10cm UV tray, 2 x 16 sample combs, loading guides and dams

 

MultiSUB Choice

Product Description

The MultiSUB Choice offers a wide degree of versatility. Three tray options are available; 15 x 7cm, 15 x 10cm and 15 x 15cm  – allowing the choice of one, two or all three gel length options at the time of purchase. Further purchases of additional accessories are no longer required. Maximising comb and tray options allow up to 210 samples to be resolved per gel. The 15cm total run length allows restriction fragment or other close MW sample bands to be easily separated and identified. Speed loading is accomplished using 10, 14, 16, 18, 28 or 30 sample multi-channel pipette compatible combs.

Fabricated MultiSUB choice stretch units are available with optional 15 x 20cm and 15 x 25cm gel trays and four 28-sample combs for those researchers wanting to perform higher resolution separation of more samples over a longer distance. MultiSUB Choice Trio includes all 3 tray sizes for optimum versatility and value.

Variants:

• MSCHOICE7 - 15 x 7cm UV tray, 2 x 20 sample combs, loading guides and dams

• MSCHOICE10 - 15 x 10cm UV tray, 2 x 20 sample combs, loading guides and dams

• MSCHOICE15 - 15 x 15cm UV tray, 2 x 20 sample combs, loading guides and dams

• MSCHOICETRIO - 15 x 7,10 & 15cm UV tray, 2 x 20 sample combs, loading guides and dams

• MSCHOICETRIO15 - 3 x 15cm UV tray, 2x 20 sample combs, loading guides and dams

• MSCHOICEST20 - 15 x 20cm UV tray, 4 x 28 sample combs, loading guides and dams
  MSCHOICEST25 - 15 x 25cm UV tray, 4 x 28 sample combs, loading guides and dams

 

MultiSUB Maxi

Product Description

The MultiSUB Maxi is primarily designed for resolution of high numbers of samples, such as from Cloning or PCR. The MultiSUB Maxi allows ultra high-resolution separations over extended runs. Tray sizes correspond to standard blotter sizes. It also allows easy sample transfer onto a membrane for further analysis.

Four gel tray sizes are available; 20 x 10cm, 20 x 15cm, 20 x 20cm and 20 x 25cm. Multichannel pipette compatible combs of up to 40 samples facilitate speed loading of up to 440 samples per gel. 50 sample combs allow maximum sample capacity of 550 samples per gel. Casting dams allow gels to be rapidly cast externally while the MultiSub unit is in use.

Variants:

• MSMAXI10 - 20 x 10cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading guides

• MSMAXI15 - 20 x 15cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading guides

• MSMAXI20 - 20 x 20cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading guides

• MSMAXIDUO - 20 x 10 & 20 x 20cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading guides

MSMAXI25 - 20 x 25cm UV Tray, 2 x 20 sample, 1mm thick combs, casting dams, loading guides

 

MultiSUB Screen

Product Description

Designed for rapid screening of very large numbers of Clonal or PCR samples, the MultiSUB Screen horizontal gel unit has a 672 maximum sample capacity per gel. This allows loading and analysis of seven 96-well format microtitre plates. The large gel run length of 32cm also allows resolution of samples over a long distance for separation of complex sample bands, such as in restriction fragment analysis.

The unit is available with a full length tray or with other tray length options of 16 or 24cm so that the user’s exact requirements can be matched. In addition to options for single length gel trays, the MultiSUB Screen is available with all three gel tray lengths to provide the maximum in flexibility, versatility and value. Buffer recirculation ports are included as standard to allow enhanced resolution over extended runs while loading guides enhance well visibility for easy sample loading.

Variants:

• MSSCREEN32 – MultiSUB Screen, 26 x 32cm UV Tray, 6 x 28 sample combs, loading guides & flexicaster

• MSSCREEN24 – MultiSUB Screen, 26 x 24cm UV Tray, 6 x 28 sample combs, loading guides & flexicaster

• MSSCREEN16 - MultiSUB Screen, 26 x 16cm UV Tray, 6 x 28 sample combs, loading guides & flexicaster

• MSSCREENTRIO - MultiSUB Screen, 26 x 16cm, 26 x 24cm, 26 x 32cm UV Trays, 6 x 28 sample combs, loading guides & flexicaster

 

MultiSUB Midi-96

 

Product Description

 

The MultiSUB Midi 96 Gel System is ideal for high throughput electrophoresis of PCR products or samples containing a small number of DNA bands. Its 10x12cm (W x L) gel dimensions and 96-well comb block format correspond to the standard microplate configuration. Two different types of comb are available; one with 8 wells and one additional lane for DNA markers, and one with 8 wells and 2 additional lanes for markers. The offset layout of the wells allows a maximum run length of 1.8cm per well, resulting in improved separation.

 

The MultiSUB Midi 96 can accommodate samples from a 96-well plate and samples can be loaded directly using 8-channel pipettes. As with all MultiSUB units, the leak-proof casting is simplified using the supplied casting dams, while their ultra-compact size minimises buffer usage and bench space requirements.

 

MSMIDI96ST Stretch Systems are also available for those users requiring an extended run length per well of up to 3.6cm, or for loading of samples from two 96-well plates – MSMIDI96STDBL.

 

Variants:

 

• MSMIDI96 - Multi Sub Midi96, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams

 

• MSMIDI961.5 - Multi Sub Midi96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have one marker lane

 

• MSMIDI96/2M - Multi Sub Midi96, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams. Combs have two marker lanes

 

• MSMIDI96/1.5/2M - Multi Sub Midi96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have two marker lanes

 

• MSMIDI96ST - Multi Sub Midi96 STRETCH, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams

 

• MSMIDI96ST1.5 - Multi Sub Midi96 STRETCH, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have one marker lane

 

• MSMIDI96ST/2M - Multi Sub Midi96 STRETCH, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams. Combs have two marker lanes

 

• MSMIDI96ST/1.5/2M - Multi Sub Midi96 STRETCH, UV tray , Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have two marker lanes

 

• MSMIDI96STDBL - Multi Sub Midi96 STRETCH, UV tray, Comb block with 24 x 8 sample, 1mm thick combs, casting dams – N.B. run length = 1.8cm.

 

MultiSUB-4

 

Product Description

The MultiSUB-4 is a compact system capable of running a maximum 1200 samples simultaneously by stacking up to 4 horizontal gels. This achieves high sample throughput while maintaining the time and space saving advantages of a small, compact unit.

Each MultiSUB-4 is supplied with 4 gel trays and 8 combs as standard, while additional trays and combs may be purchased as required. Two double-sided comb and three tray length formats are also available. An optional flexicaster, either supplied separately or as a package with the MultiSUB-4, is also available that allows up to four 8×6, 8×12 and 8x18cm multiSUB-4 gel trays to be cast simultaneously outside the tank.

Variants:

• CSL-MULTISUB4 - Multisub4, Multi-Level Gel Chamber, Includes 4x 18cm UV Trays, 8x 18/8 Sample 1.5mm Combs (Tape UV Trays To Seal)

• CSL-MULTISUB4EXCAS - Multisub4, as above but with External Caster for 4 Gels

 

MiniRAPIDE

 

Product Description

 

The MiniRapide is a completely self contained system designed for quick checks of samples. Gel casting, running and analysis are all performed in the same ultra compact unit. Buffer and gel volumes have been kept to a minimum and the parallel electrode arrangement allows ultra efficient current transfer, enabling resolution to be completed within 30 minutes. The UV transparent base allows direct viewing on a UV Transilluminator with no need for time consuming transfer and potential gel damage. Dual comb slots allow the loading of up to 40 samples per gel, while multichannel pipette compatible combs further enhance the speed and convenience.

 

Variants:

 

• FMMS10 – MiniRapide 10 x 8cm, 2 x 8 sample combs 1.5mm and casting dams