Isoelectric Focusing

Gentaur IPG Strips are available in 3 different strips lengths to fit all Gentaur vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system.

Ampholytes (2ml Volume)

Product Description

Ampholytes are synthetically derived, low molecular weight zwitterionic molecules, comprising a multitude of pI values. Because of their capacity to carry both positive and negative charge, they are used to make capillary ampholyte gels and IPG strips. A linear pH-gradient is generated when an electric field is applied and they migrate to the position of their corresponding pI (isoelectric point) and have no resultant net charge. Gentaur recommends the application of ampholytes in IPG strip rehydration.

Ampholytes (2ml Volume) recommended for strip rehydration.

Variants:

CSL-AMP3-10/2 - pH Range 3-10

CSL-AMP3-10/2ID - pH Range 3-10 Iso-Dalt

CSL-AMP3-6/2 - pH Range 3-6

CSL-AMP4-7/2 - pH Range 4-7

CSL-AMP5-8/2 - pH Range 5-8

CSL-AMP6-9/2 - pH Range 6-9

IPG Strips pH Range 6-10

Product Description

IPG strips are available in 3 different strips lengths to fit all Gentaur vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system. Shorter strips are normally used to develop and validate methods in the omniPAGE CVS10 mini-gel system*, whereas longer strips of 18cm lengths are best employed in the VS20 WAVE maxi system, with its IPG converter kit, to optimise separation and resolution with higher protein loads. 24cm IPG strips are recommended for use in the VS30 second-dimension vertical system. Gentaur IPG strips are cast using high quality ampholytes, and undergo stringent QC tests for quality and 2-D gel-to-gel reproducibility.

*May also be used with VS10WDSYS with its IPG converter kit.

Design benefits offered by Gentaur IPG strips include:

A rigid plastic backing that supports a covalently bound gel matrix to prevent breakage and make handling easier

Each strip is supplied freeze-dried and with a detachable cover film to stop contamination

Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance

The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE

Light blue stain migrates from cathode to anode during focusing, serving as a marker for electrophoresis progression

Sample-loading by overnight rehydration or cup-loading

Gradient selection criteria:

Supplied in 7, 18 and 24cm strip lengths with 6 different gradients

Standard broad-range pH gradients maximise resolution within a single second-dimension gel (e.g. pH 3-10)

Narrow-range gradients (e.g. pH 3-6) optimise resolving power within a specific pH region by offering more centimetres of gel per pH unit

pH 3-10 Non Linear gradient strips have a flat zone corresponding to pH 5-7 for increased protein resolving power within this region

Quality assured:

Consistency – all 12 strips supplied with each package come from the same production lot

Reliability – accurate pH gradient casting procedures ensure lot-to-lot and 2-D gel-to-gel reproducibility

May be used with all commercial IEF systems

Variants:

CSL-IPG6-10/7 - 7cm IPG Strip Length

CSL-IPG6-10/18 - 18cm IPG Strip Length

CSL-IPG6-10/24 - 24cm IPG Strip Length

IPG Strips pH Range 5-8

Product Description

IPG strips are available in 3 different strips lengths to fit all Gentaur vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system. Shorter strips are normally used to develop and validate methods in the omniPAGE CVS10 mini-gel system*, whereas longer strips of 18cm lengths are best employed in the VS20 WAVE maxi system, with its IPG converter kit, to optimise separation and resolution with higher protein loads. 24cm IPG strips are recommended for use in the VS30 second-dimension vertical system. Gentaur IPG strips are cast using high quality ampholytes, and undergo stringent QC tests for quality and 2-D gel-to-gel reproducibility.

*May also be used with VS10WDSYS with its IPG converter kit.

Design benefits offered by Gentaur IPG strips include:

A rigid plastic backing that supports a covalently bound gel matrix to prevent breakage and make handling easier

Each strip is supplied freeze-dried and with a detachable cover film to stop contamination

Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance

The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE

Light blue stain migrates from cathode to anode during focusing, serving as a marker for electrophoresis progression

Sample-loading by overnight rehydration or cup-loading

Gradient selection criteria:

Supplied in 7, 18 and 24cm strip lengths with 6 different gradients

Standard broad-range pH gradients maximise resolution within a single second-dimension gel (e.g. pH 3-10)

Narrow-range gradients (e.g. pH 3-6) optimise resolving power within a specific pH region by offering more centimetres of gel per pH unit

pH 3-10 Non Linear gradient strips have a flat zone corresponding to pH 5-7 for increased protein resolving power within this region

Quality assured:

Consistency – all 12 strips supplied with each package come from the same production lot

Reliability – accurate pH gradient casting procedures ensure lot-to-lot and 2-D gel-to-gel reproducibility

May be used with all commercial IEF systems

Variants:

CSL-IPG5-8/7 - 7cm IPG Strip Length

CSL-IPG5-8/18 - 18cm IPG Strip Length

CSL-IPG5-8/24 - 24cm IPG Strip Length

Gentaur IPG Strips pH Range 4-7

Product Description

Gentaur IPG strips are available in 3 different strips lengths to fit all Gentaur vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system. Shorter strips are normally used to develop and validate methods in the omniPAGE CVS10 mini-gel system*, whereas longer strips of 18cm lengths are best employed in the VS20 WAVE maxi system, with its IPG converter kit, to optimise separation and resolution with higher protein loads. 24cm IPG strips are recommended for use in the VS30 second-dimension vertical system. Gentaur IPG strips are cast using high quality ampholytes, and undergo stringent QC tests for quality and 2-D gel-to-gel reproducibility.

*May also be used with VS10WDSYS with its IPG converter kit.

Design benefits offered by Gentaur IPG strips include:

A rigid plastic backing that supports a covalently bound gel matrix to prevent breakage and make handling easier

Each strip is supplied freeze-dried and with a detachable cover film to stop contamination

Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance

The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE

Light blue stain migrates from cathode to anode during focusing, serving as a marker for electrophoresis progression

Sample-loading by overnight rehydration or cup-loading

Gradient selection criteria:

Supplied in 7, 18 and 24cm strip lengths with 6 different gradients

Standard broad-range pH gradients maximise resolution within a single second-dimension gel (e.g. pH 3-10)

Narrow-range gradients (e.g. pH 3-6) optimise resolving power within a specific pH region by offering more centimetres of gel per pH unit

pH 3-10 Non Linear gradient strips have a flat zone corresponding to pH 5-7 for increased protein resolving power within this region

Quality assured:

Consistency – all 12 strips supplied with each package come from the same production lot

Reliability – accurate pH gradient casting procedures ensure lot-to-lot and 2-D gel-to-gel reproducibility

May be used with all commercial IEF systems

Variants:

CSL-IPG4-7/7 - 7cm IPG Strip Length

CSL-IPG4-7/18 - 18cm IPG Strip Length

CSL-IPG4-7/24 - 24cm IPG Strip Length

Gentaur IPG Strips pH Range 3-6

Product Description

Gentaur IPG strips are available in 3 different strips lengths to fit all Gentaur vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system. Shorter strips are normally used to develop and validate methods in the omniPAGE CVS10 mini-gel system*, whereas longer strips of 18cm lengths are best employed in the VS20 WAVE maxi system, with its IPG converter kit, to optimise separation and resolution with higher protein loads. 24cm IPG strips are recommended for use in the VS30 second-dimension vertical system. Gentaur IPG strips are cast using high quality ampholytes, and undergo stringent QC tests for quality and 2-D gel-to-gel reproducibility.

*May also be used with VS10WDSYS with its IPG converter kit.

Design benefits offered by Gentaur IPG strips include:

A rigid plastic backing that supports a covalently bound gel matrix to prevent breakage and make handling easier

Each strip is supplied freeze-dried and with a detachable cover film to stop contamination

Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance

The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE

Light blue stain migrates from cathode to anode during focusing, serving as a marker for electrophoresis progression

Sample-loading by overnight rehydration or cup-loading

Gradient selection criteria:

Supplied in 7, 18 and 24cm strip lengths with 6 different gradients

Standard broad-range pH gradients maximise resolution within a single second-dimension gel (e.g. pH 3-10)

Narrow-range gradients (e.g. pH 3-6) optimise resolving power within a specific pH region by offering more centimetres of gel per pH unit

pH 3-10 Non Linear gradient strips have a flat zone corresponding to pH 5-7 for increased protein resolving power within this region

Quality assured:

Consistency – all 12 strips supplied with each package come from the same production lot

Reliability – accurate pH gradient casting procedures ensure lot-to-lot and 2-D gel-to-gel reproducibility

May be used with all commercial IEF systems

Variants:

CSL-IPG3-6/7 - 7cm IPG Strip Length

CSL-IPG3-6/18 - 18cm IPG Strip Length

CSL-IPG3-6/24 - 24cm IPG Strip Length

Gentaur IPG Strips pH Range 3-10 NL

Product Description

Gentaur IPG strips are available in 3 different strips lengths to fit all Gentaur vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system. Shorter strips are normally used to develop and validate methods in the omniPAGE CVS10 mini-gel system*, whereas longer strips of 18cm lengths are best employed in the VS20 WAVE maxi system, with its IPG converter kit, to optimise separation and resolution with higher protein loads. 24cm IPG strips are recommended for use in the VS30 second-dimension vertical system. Gentaur IPG strips are cast using high quality ampholytes, and undergo stringent QC tests for quality and 2-D gel-to-gel reproducibility.

*May also be used with VS10WDSYS with its IPG converter kit.

Design benefits offered by Gentaur IPG strips include:

A rigid plastic backing that supports a covalently bound gel matrix to prevent breakage and make handling easier

Each strip is supplied freeze-dried and with a detachable cover film to stop contamination

Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance

The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE

Light blue stain migrates from cathode to anode during focusing, serving as a marker for electrophoresis progression

Sample-loading by overnight rehydration or cup-loading

Gradient selection criteria:

Supplied in 7, 18 and 24cm strip lengths with 6 different gradients

Standard broad-range pH gradients maximise resolution within a single second-dimension gel (e.g. pH 3-10)

Narrow-range gradients (e.g. pH 3-6) optimise resolving power within a specific pH region by offering more centimetres of gel per pH unit

pH 3-10 Non Linear gradient strips have a flat zone corresponding to pH 5-7 for increased protein resolving power within this region

Quality assured:

Consistency – all 12 strips supplied with each package come from the same production lot

Reliability – accurate pH gradient casting procedures ensure lot-to-lot and 2-D gel-to-gel reproducibility

May be used with all commercial IEF systems

Variants:

CSL-IPG3-10/7NL - 7cm IPG Strip Length

CSL-IPG3-10/18NL - 18cm IPG Strip Length

CSL-IPG3-10/24NL - 24cm IPG Strip Length

Gentaur IPG Strips pH Range 3-10

Product Description

Gentaur IPG strips are available in 3 different strips lengths to fit all Gentaur vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system. Shorter strips are normally used to develop and validate methods in the omniPAGE CVS10 mini-gel system*, whereas longer strips of 18cm lengths are best employed in the VS20 WAVE maxi system, with its IPG converter kit, to optimise separation and resolution with higher protein loads. 24cm IPG strips are recommended for use in the VS30 second-dimension vertical system. Gentaur IPG strips are cast using high quality ampholytes, and undergo stringent QC tests for quality and 2-D gel-to-gel reproducibility.

*May also be used with VS10WDSYS with its IPG converter kit.

Design benefits offered by Gentaur IPG strips include:

A rigid plastic backing that supports a covalently bound gel matrix to prevent breakage and make handling easier

Each strip is supplied freeze-dried and with a detachable cover film to stop contamination

Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance

The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE

Light blue stain migrates from cathode to anode during focusing, serving as a marker for electrophoresis progression

Sample-loading by overnight rehydration or cup-loading

Gradient selection criteria:

Supplied in 7, 18 and 24cm strip lengths with 6 different gradients

Standard broad-range pH gradients maximise resolution within a single second-dimension gel (e.g. pH 3-10)

Narrow-range gradients (e.g. pH 3-6) optimise resolving power within a specific pH region by offering more centimetres of gel per pH unit

pH 3-10 Non Linear gradient strips have a flat zone corresponding to pH 5-7 for increased protein resolving power within this region

Quality assured:

Consistency – all 12 strips supplied with each package come from the same production lot

Reliability – accurate pH gradient casting procedures ensure lot-to-lot and 2-D gel-to-gel reproducibility

May be used with all commercial IEF systems

Variants:

CSL-IPG3-10/7 - 7cm IPG Strip Length

CSL-IPG3-10/18 - 18cm IPG Strip Length

CSL-IPG3-10/24 - 24cm IPG Strip Length

 

Horizontal Nucleic Acid Electrophoresis

High Grade Agarose for Nucleic Acid Electrophoresis. Clever for DNA and Clever for the Environment

CleverGEL is a new environmentally friendly agarose suitable for routine analysis of nucleic acids using standard electrophoretic procedures. CleverGEL is manufactured to exclude organic solvents harmful to marine life, making it far kinder to the environment than conventional agarose.

runSafe

Product Description

runSafe – A new ready to use safe and sensitive alternative to ethidium bromide and other DNA stains!

runSafe is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of gels. Supplied in Gentaur’s 6X DNA Loading Buffer, runSafe is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. runSafe is a highly sensitive stain available for detecting doublestranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visual tracking of DNA migration during electrophoresis. It is ideal for the environment providing a safe, sensitive,
non-mutagenic and non-hazardous alternative to Ethidum Bromide

See your samples separate in real time

Simply mix, load and run – runSafe acts as both a loading dye and a DNA stain combined for added speed and convenience. Economic and Low environmental impact – No expensive waste management is required and complies with Clean Water Act standards. runSafe – Supplied in 6X Loading Buffer

• Variant:

• CSL-RUNSAFE: 1 ml/ vial

 

Agarose Low EEO

Product Description

CleverGEL is a new environmentally friendly agarose suitable for routine analysis of nucleic acids using standard electrophoretic procedures. CleverGEL is manufactured by a process which excludes organic solvents harmful to marine life, making it far kinder to the environment than conventional agarose. A low EEO (electroendoosmotic) flow minimises diffusion so that even the smallest of nucleic acid fragments remains sharp and tightly resolved, while a high gel strength aids handling and maintains compatibility with blotting techniques. CleverGEL is now available in a low melting point form for nucleic acid recovery and enzymatic applications, as well as in a high resolution PCR-grade form to resolve very small nucleic acid fragments 20-800bp in size.

Variants:

• CSL-AG100 - Agarose 100g, Low EEO

• CSL-AG500 - Agarose 500g, Low EEO

• CSL-AG1000 - Agarose 1000g, Low EEO (2x500g bottles)

• CSL-AG2000 - Agarose 2000g, Low EEO (4x500g bottles)

• CSL-AG5000 - Agarose 5000g, Low EEO (10x500g bottles)

• CSL-AG10KG - Agarose 10kg, Low EEO (20x500g bottles)

• CSL-AG20KG - Agarose 20KG, Low EEO (40x500g bottles)

 

Agarose Low Melting Point

Product Description

CleverGEL is a new environmentally friendly agarose suitable for routine analysis of nucleic acids using standard electrophoretic procedures. CleverGEL is manufactured by a process which excludes organic solvents harmful to marine life, making it far kinder to the environment than conventional agarose. A low EEO (electroendoosmotic) flow minimises diffusion so that even the smallest of nucleic acid fragments remains sharp and tightly resolved, while a high gel strength aids handling and maintains compatibility with blotting techniques. CleverGEL is now available in a low melting point form for nucleic acid recovery and enzymatic applications, as well as in a high resolution PCR-grade form to resolve very small nucleic acid fragments 20-800bp in size.

Variants:

• CSL-LMA5 – Agarose 5g, LMP

• CSL-LMA50 – Agarose 50g, LMP

• CSL-LMA100 - Agarose 100g, LMP

• CSL-LMA500 - Agarose 500g, LMP

 

Agarose High Resolution PCR-Grade

Product Description

CleverGEL is a new environmentally friendly agarose suitable for routine analysis of nucleic acids using standard electrophoretic procedures. CleverGEL is manufactured by a process which excludes organic solvents harmful to marine life, making it far kinder to the environment than conventional agarose. A low EEO (electroendoosmotic) flow minimises diffusion so that even the smallest of nucleic acid fragments remains sharp and tightly resolved, while a high gel strength aids handling and maintains compatibility with blotting techniques. CleverGEL is now available in a low melting point form for nucleic acid recovery and enzymatic applications, as well as in a high resolution PCR-grade form to resolve very small nucleic acid fragments 20-800bp in size.

Variants:

• CSL-HRA100 - Agarose 100g, HR

• CSL-HRA500 - Agarose 500g, HR

 

Powdered and Liquid Buffers

Product Description

Gentaur supplies TBE and TAE in powder and liquid form. Both TBE and TAE are widely used to separate nucleic acids in horizontal gel electrophoresis, and occasionally in vertical polyacrylamide gels. TBE has a higher buffering capacity than TAE, which is used for faster separations of linear double-stranded DNA.

Gentaur TBE is supplied in packs of 10 powder sachets to maintain shelf life. Each buffer sachet may be opened as required and reconstituted in distilled water to make 1 litre of working solution. Buffers are also provided as ready-made 50x TAE and 10x TBE solutions in 1 and 4 litre volumes. These are ideal for laboratories running horizontal nucleic acid gels on a daily basis that require off-the-shelf working stock solutions.

Variants:

• TBE10X1L –  Tris-Borate-EDTA Running Buffer, 10 x 1L

• TBE10X5L - Tris-Borate-EDTA Running Buffer, 10 x 5L

• TAE50X1L - Tris-Acetate-EDTA Running Buffer, 50 x 1L

• TAE50X5L - Tris-Acetate-EDTA Running Buffer, 50 x 5L

• CSL-TBEP - Powdered Tris-Borate-EDTA Running Buffer, 10 sachets (10 litres/pack)

 

DNA Markers

Product Description

Pre-made and containing loading dye for immediate use, Gentaur’s ready-to-use (RTU) DNA Markers are specially formulated to run accurately and produce sharp, well defined ladders. Available in six molecular weight ranges and composed of discrete marker fragments isolated from restriction-digested proprietary plasmids, each DNA marker will remain stable for up to 6 months at room temperature and 12 months if kept in the fridge at 4.  All Gentaur RTU Markers contain high intensity reference bands and may be used to perform size comparisons with DNA molecules ranging from the smallest of PCR fragments to large, linearised cosmid vectors.

Variants:

• CSL-MDNA-100BPH - 100bp DNA ladder, 100-3000bp, 1x500ul vial

• CSL-MDNA-100BP - 100bp DNA ladder, 100-1500bp, 1x500ul vial

• CSL-MDNA-1KB - 1KBbp DNA ladder, 250-10kb, 1x500ul vial

• CSL-MDNA-50BP - 50bp DNA ladder, 50-1500bp, 1x500ul vial

• CSL-MDNA-BR - Broad Range DNA ladder, 100bp-1oKb, 1x500ul vial

• CSL-MDNA-HR - High Range DNA ladder, 250bp-25Kb, 1x500ul vial

 

DNA Loading Dye

Product Description

DNA Loading Dye contains 10x bromophenol blue, the standard tracking dye for electrophoresis. The charge-to-mass ratio of bromophenol blue allows it to co-migrate with smaller molecules within agarose and PAGE gels (e.g. at 300bp in a standard 1% agarose TBE gel) which, with its conspicuous dark blue colour, makes it the perfect tracking dye to monitor the progress of electrophoresis runs.

DNA Loading Dye is supplied in 1ml volumes for easy handling.

10x Bromophenol Blue Loading Dye, 1ml

Variant:

CSL-LOADDYE

 

RNase-Free Water

Product Description

DEPC-treated to eliminate enzyme activity and then autoclaved, this sterile highly purified water product is perfect for use in PCR and Northern blotting techniques. RNase-Free water is available either as a single 250ml bottle or in fifty 5ml aliquots to prevent cross-contamination.

Variants:

• RFW250 - RNase-Free Water, 1x250ml

• RFW50X5 - RNase-Free Water, 50x5ml

 

GelX Disposable Gel Excision Tips

Product Description

Widely acclaimed as an excellent tool for gene cloning, Gentaur GelX Disposable Gel Excision Tips offer a unique and convenient way to excise nucleic acid and protein bands from gels. GelX tips allow completely safe and contamination free removal of the gel band of interest without the need to handle potentially dangerous scalpels. The gel band is neatly extracted and contained in the tip, where it can then be dispensed into a tube for further analysis. Once used, the tip is ejected for disposal.

• Eliminates scalpel damage to transilluminator or gel tray

• Designed for removing bands from agarose and protein gels

• One-handed operation

• Push button gel and tip release mechanism

• Fits standard 1000ul pipettes

• Autoclavable, and DNase and RNase free

• Sterile versions also available

Variants:

• CSL-GELX4 – 4mm x 1mm, Gel Cutting Tips, 250/ bag

• CSL-GELX4RACK – 4mm x 1mm, Gel Cutting Tips, 5 racks of 48

• CSL-GELX6.5 – 6.5mm x 1mm, Gel Cutting Tips, 250/bag

• CSL-GELX6.5RACK - 6.5mm x 1mm, Gel Cutting Tips, 5 racks of 48

 

 

 

Vertical Protein Electrophoresis

Protein markers, Pre cast Gels and Buffers for Vertical electrophoreis.

Pre Cast Gels

Product Description

Gentaur’s PAGE-PRO composite gels are ideally suited to all SDS-PAGE applications. Each gel has a minimum 12 month shelf life that is guaranteed by a specially formulated, proprietary gel matrix composition, which also provides superior performance compared to traditional Laemmli gels.

Available in single and gradient percentage acrylamide compositions, PAGE-PRO gels are manufactured by an automated filling technique that ensures total reproducibility between batches. PAGE-PRO gels may be used with standard Tris-MOPS, Tris-HEPES and Tris Tricine SDS running buffers and are suitable for use with our omniPAGE mini vertical electrophoresis and blotting systems.

Variant:

• PPRO42010 - PAGE-PRO Gradient 4-20% 10 Well, 10 Pack (10x10cm)

• PPRO42015 - PAGE-PRO  Gradient 4-20% 15 Well, 10 Pack (10x10cm)

• PPRO1610 - PAGE-PRO 16% 10 Well, 10 Pack (10x10cm)

• PPRO1615 - PAGE-PRO  16% 15 Well, 10 Pack (10x10cm)

 

Protein Ladder –  Prestained

Product Description

Adding colour to your PAGE

Stable for up to 2 years if stored at -20C and supplied pre-stained in gel loading buffer for direct loading, Gentaur PINK Plus and BLUE Wide Range recombinant protein markers are perfect for SDS- PAGE applications. Sizes range from 10-175kDa for PINK Plus and 10-245kDa for BLUE Wide Range, making both markers suitable for accurate molecular weight determination of most cellular proteins.

Each marker is covalently bound to a pink or blue colour chromaphore to produce a ladder of evenly interspersed bands of uniform intensity. Coloured reference bands serve as visual indicators of electrophoresis run progression and the efficiency of western transfer onto membranes following SDS-PAGE. Both PINK Plus and BLUE Wide Range markers can be detected at volumes as low as 2.5µl per well.

Variant:

• CSL-PPL – Pink Plus Prestained Protein ladder (10-175kDa)

• CSL-BBL - Blue Wide Range Protein Ladder (10-245kDa)

 

PAGE Buffers

Product Description

Five buffers are available in powder sachets for a range of native and denaturing protein gel electrophoresis techniques. Each powder sachet, which is supplied as a 10-pack, may be reconstituted to make 1 litre of working solution. Running buffers are also available in 1 litre and 5 litre volumes as ready-made 10x Tris-Glycine and 10x Tris-Glycine-SDS solutions.

Variants:

• CSL-TGSDSP – Powdered Tris-Glycerine-SDS Running Buffer 10 sachets (10 litres/pk)

• CSL-TGP - Powdered Tris-Glycerine Running Buffer 10 sachets (10 litres/pk)

• CSL-TTSDSP - Powdered Tris-Tricine-SDS Running Buffer 10 sachets (10 litres/pk)

• CSL-MSDSP - Powdered MOPS-SDS Running Buffer 10 sachets (10 litres/pk)

• CSL-MESDSP - Powdered MES-SDS Running Buffer 10 sachets (10 litres/pk)

• TG10X1L - Cleaver Buffer Tris-Glycerine 10x 1 litre

• TG10X5L -  Cleaver Buffer Tris-Glycerine 10x 5 litre

• TG-SDS10X1L - Cleaver Buffer Tris-Glycine SDS 10 x 1 litre

• TG-SDS10X5L - Cleaver Buffer Tris-Glycine SDS 10 x 5 litre