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    Thursday, 05 December 2013 13:26

    JETSTAR 2.0 Maxi

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    Description:

    JETSTAR is a new and unique anion exchange resin
    developed by Gentaur. This new resin is supplied in disposable
    columns (Mini, Midi and Maxi columns) , which are used
    under gravity flow conditions without further instrumentation.
    The JETSTAR Plasmid Kits were designed by combining
    JETSTAR Columns with a modified alkaline/SDS lysis proce-
    dure for the preparation of plasmid DNA. Gentaur´s product
    is the ideal tool to achieve ultrapure plasmid DNA within 45

    Procedure :The procedure employs a modified alkaline/SDS method to prepare the cleared lysate. After neutral-ization, the lysate is applied onto a JETSTAR Column (Mini, Midi or Maxi) and the plasmid DNA is bound to the anion exchange resin . Washing the resin removes RNA and all other impurities. Finally the purified plasmid DNA is eluted from the column and concentrated by an alcohol precipitation. 

    Plasmid yields :Yields of up to 20 µg (Mini), 100 µg (Midi) and 500 µg (Maxi) of plasmid DNA can be expected using the JETSTAR columns. All types and sizes of plasmid DNA can be prepared, but yields depend very much on the plasmid copy numbers (low/medium/high), the type  f plasmid, the bacterial strain and the volume of bacterial culture used. The recovery of plasmid DNA is on average between 85% and 90%.

    Plasmid purity :The JETSTAR-purified plasmid DNA is of higher quality than 2 x CsCl purified plasmid DNA. Corre- spondingly, its recommended application range is extremely wide, including transfection, microinjection, vaccination, gene therapy, fluorescent and radioactive sequencing, amplification, in vitro transcription, cloning, etc.

    Culture V olumes and DNA Yields
    JETSTAR Kits are preferentially designed to extract and purify high copy plasmid DNA from E.coli cultures. Low copy plasmids can be prepared as well, but larger culture volumes have to be used.
    Gentaur recommends LB medium to grow E.coli cells to prepare plasmid DNA with JETSTAR columns. The JETSTAR system is however compatible also with other growth media, especially those designed for enhanced plasmid yields . The cell density should be approximately 1 x 10 cells per ml medium (1-1.5 A 600 units/ml).


    High Copy Plasmids (3 - 5 µg DNA/ml LB medium)

    A. Culture Volume DNA Yield
    Mini 1 -5 ml 3 - 20 µg
    Midi 15 - 25 ml 45 - 100 µg
    Maxi 100 ml 300 - 500 µg

     

    Low Copy Plasmids   (0.2 - 1 µg DNA/ml LB medium)

    A. Culture Volume DNA Yield
    Mini 10 - 15 ml 2 - 45 µg
    Midi 25 - 100 ml 5 - 100 µg
    Maxi 250 - 500 ml 50 - 500 µg

     

    Product pricelist:

    Product Contents Cat.# Price:
    JETSTAR 2.0 Plasmid Midiprep Kit / 25 25 Midi Columns
    Solutions, Reagents
    2210025 126 €
    JETSTAR 2.0 Plasmid Midiprep Kit / 50 200 Midi Columns
    Solutions, Reagents
    210050 202 €
    JETSTAR 2.0 Plasmid Midiprep Kit / 250 250 Midi Columns
    Solutions, Reagents
    210250 721 €
    JETSTAR 2.0 Plasmid Midiprep LFU Kit / 25 50 Midi Columns
    Solutions, Reagents
    211025 186 €
    JETSTAR 2.0 Plasmid Midiprep LFU Kit / 100 10 Midi Columns
    Solutions, Reagents
    211100 547 €
    JETSTAR 2.0 Plasmid Maxiprep Kit / 10 10 Maxi Columns
    Solutions, Reagents
    220010 126 €
    JETSTAR 2.0 Plasmid Maxiprep Kit / 20 20 Maxi Columns
    Solutions, Reagents
    220020 202 €
    JETSTAR 2.0 Plasmid Maxiprep Kit / 100 100 Maxi Columns
    Solutions, Reagents
    220100 769 €
    JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 20 20 Maxi Columns
    Solutions, Reagents
    221020 289 €

     

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