| GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45
Fax 0032 16 50 90 45
GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
GENTAUR SRL IVA IT03841300167
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Fax 0032 16 50 90 45
Schweiz Züri +41435006251
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
RETRO-TEK kits have been designed for the quantitative measurement of viral antigens from the retroviruses HIV-1, SIV and HTLV found in cell culture, serum, plasma or other biological fluids.
Cat Number: 9621
More information: Sterile, individually wrapped
Description: CLP supplies an 8-port manifold which has a friction slip fit on to the 50ml combi-syringe. The tips on the manifold follow the standard 9mm center microplate spacing. With aqueous solutions this tip will provide equal dispensing across all 8 tips to within 1.7% of the dispensed volume. Because of accuracy concerns across larger diameters, CLP does not supply a twelve-port manifold.
Price: 99 Euro
JETSTAR is a new and unique anion exchange resin
developed by Gentaur. This new resin is supplied in disposable
columns (Mini, Midi and Maxi columns) , which are used
under gravity flow conditions without further instrumentation.
The JETSTAR Plasmid Kits were designed by combining
JETSTAR Columns with a modified alkaline/SDS lysis proce-
dure for the preparation of plasmid DNA. Gentaur´s product
is the ideal tool to achieve ultrapure plasmid DNA within 45
Procedure :The procedure employs a modified alkaline/SDS method to prepare the cleared lysate. After neutral-ization, the lysate is applied onto a JETSTAR Column (Mini, Midi or Maxi) and the plasmid DNA is bound to the anion exchange resin . Washing the resin removes RNA and all other impurities. Finally the purified plasmid DNA is eluted from the column and concentrated by an alcohol precipitation.
Plasmid yields :Yields of up to 20 µg (Mini), 100 µg (Midi) and 500 µg (Maxi) of plasmid DNA can be expected using the JETSTAR columns. All types and sizes of plasmid DNA can be prepared, but yields depend very much on the plasmid copy numbers (low/medium/high), the type f plasmid, the bacterial strain and the volume of bacterial culture used. The recovery of plasmid DNA is on average between 85% and 90%.
Plasmid purity :The JETSTAR-purified plasmid DNA is of higher quality than 2 x CsCl purified plasmid DNA. Corre- spondingly, its recommended application range is extremely wide, including transfection, microinjection, vaccination, gene therapy, fluorescent and radioactive sequencing, amplification, in vitro transcription, cloning, etc.
Culture V olumes and DNA Yields
JETSTAR Kits are preferentially designed to extract and purify high copy plasmid DNA from E.coli cultures. Low copy plasmids can be prepared as well, but larger culture volumes have to be used.
Gentaur recommends LB medium to grow E.coli cells to prepare plasmid DNA with JETSTAR columns. The JETSTAR system is however compatible also with other growth media, especially those designed for enhanced plasmid yields . The cell density should be approximately 1 x 10 cells per ml medium (1-1.5 A 600 units/ml).
High Copy Plasmids (3 - 5 µg DNA/ml LB medium)
|A.||Culture Volume||DNA Yield|
|Mini||1 -5 ml||3 - 20 µg|
|Midi||15 - 25 ml||45 - 100 µg|
|Maxi||100 ml||300 - 500 µg|
Low Copy Plasmids (0.2 - 1 µg DNA/ml LB medium)
|A.||Culture Volume||DNA Yield|
|Mini||10 - 15 ml||2 - 45 µg|
|Midi||25 - 100 ml||5 - 100 µg|
|Maxi||250 - 500 ml||50 - 500 µg|
|JETSTAR 2.0 Plasmid Midiprep Kit / 25||25 Midi Columns
|JETSTAR 2.0 Plasmid Midiprep Kit / 50||200 Midi Columns
|JETSTAR 2.0 Plasmid Midiprep Kit / 250||250 Midi Columns
|JETSTAR 2.0 Plasmid Midiprep LFU Kit / 25||50 Midi Columns
|JETSTAR 2.0 Plasmid Midiprep LFU Kit / 100||10 Midi Columns
|JETSTAR 2.0 Plasmid Maxiprep Kit / 10||10 Maxi Columns
|JETSTAR 2.0 Plasmid Maxiprep Kit / 20||20 Maxi Columns
|JETSTAR 2.0 Plasmid Maxiprep Kit / 100||100 Maxi Columns
|JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 20||20 Maxi Columns
Cat. num.: BIO-27044
Quantity: 500 Units
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM).
UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over-expressed modified gene of Uracil DNA Glycosylase from E. coli.
• Removal of uracil from uracil-containing DNA
• Novel temperature sensitive mutant irreversibly inactive after
• UDG treatment of uracil-containing DNA to prevent
amplification by DNA Polymerases
• Used to investigate features of protein-DNA interactions
Storage and stability:
Uracil DNA Glycosylase (UDG) is shipped on Dry/Blue Ice. It should be stored at -20°C upon receipt. When stored under optimum conditions, the reagents are stable for 24 months from date of purchase.
Storage and Dilution Buffer:
20mM Tris-Cl, pH 8.0, 0.1M NaCl, 0.1mM EDTA, 1mM DTT, 0.1% stabilizer and 50% glycerol.
Price: 57 €
Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.
Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.
Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.
Virus Core Antigen Detection
- Measures the p24 core protein of HIV-1
- Quantitation is performed on a standard microplate reader
- p24 Standard included
Overview: An anti-HIV p24 monoclonal coating antibody is adsorbed onto a microtiter plate. p24 antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-p24 antibody is added and binds to p24 antigen captured by the first antibody.
Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-p24. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells.
A colored product is formed in proportion to the amount of p24 antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HIV-1 p24 protein and sample p24 concentration is then determined.
Storage: Store all kit reagents at 2° - 8°C. DO NOT FREEZE. When stored properly the kit is stable until the date indicated on the box label.
Price: 486 Euro
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.
Source : E.coli
Catalog number : 04-GMPhuIL4-50 µg
Molecular weight : 15 KDa
Identity : rh IL-4 as measured by Elisa and Western Blot
Specific activity : > 13.10 6 units/mg compared to NIBSC standard . CT-h4.S cell proliferation assay.
650 000 IU /vial
Purity : > 98% as determined by SDS-PAGE and HPLC
Endotoxin content : < 0.1 EU/ µg of IL-4 (LAL test)
Host DNA : < 10 pg/ng protein
Sterility : sterile according to EP Test 2.6.1.
Stabiliser : contains Trehalose ; no preservative.
Physical state : freeze-dried
Stability : 12 months at – 20°C to – 80°C
3 months after reconstitution with water for injection ( as defined in EP Monograph 0169) when stored at -80°C
Reconstitution : use 500 µL water for injection in class A environment in order to keep the GMP grade .
Packaging unit : 50 µg protein (Lowry test)
GENTAUR rh IL-4 is manufactured in full compliance with cGMP in facilities approved by the Belgian Ministry of Health for the production and storage of medicinal products.
Use: GENTAUR rh IL-4 is not an approved medicinal product and cannot be injected as such to patients.
FOR EX VIVO CELL CULTURE AND FOR IN VITRO RESEARCH USE ONLY
Price: 483 Euro
Exosomes are 60-120 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids, urine and in media from cells in culture. Exosomes contain distinct subsets of microRNAs depending upon the cell type from which they are secreted. Standard growth medium for most cells in culture require fetal bovine serum (FBS) as a growth supplement to DMEM. FBS is derived from bovine (cow) serum and contains a high abundance of cow exosome vesicles. These exosomes can interfere or cause significant background issues when studying the exosomes secreted from your cells of interest in standard culture conditions.
Exo-FBS supports equivalent growth of many types of cells in culture, is devoid of cow CD63 positive exosomes and does not have any measurable bovine microRNAs. Perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes in your experiments. No ultracentrifugation required. Available as standard FBS supplement or heat inactivated FBS media supplement (treated at 65°C for 15 minutes before bovine exosome removal).
- Exosome-sized vesicles removed
- FBS that has been stripped of CD63 positive cow exosomes
- No detectable cow microRNAs
- Same cellular growth rates supported as standard FBS
- Use the same as standard FBS (add 10% in DMEM or RPMI)
• No time-consuming ultracentrifugation
• Less expensive than costly Antibodies and beads
• No complicated syringes required
• Compatible with biofluid from any species
• More effective than any other method
• Isolate intact exosomes for functional studies
• Use as little as 100 µl of serum
• Start with 5 or 10 ml Media or Urine and use ExoQuick-TC
The Bullet Blender Gold 50 combines dry ice cooling to keep samples near 4°C, noise insulation to keep homogenization quiet, and extra-powerful homogenization for tough samples. This homogenizer for tissue or cell cultures, can process up to 8 samples in parallel, each sample up to 3.5 g each in a 50 mL tube (Axygen® or Corning® brand 50 mL self standing tube with plug-seal cap).
Includes the following:
The Bullet Blender® Gold
Basic starter kit which includes:
Bead Sample Pack BSP-50B contains 20mL centrifuge tubes of several different types of beads
Spoon for dispensing beads***
Price: 4985 Euro
More form Gentaur: Order Beads for Bullet Blender