Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA
    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

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    Targatt services
    Monday, 11 March 2013 15:53

    Retrovirus Packaging

    We provide high quality Retrovirus packing services.


    Retroviral vectors are one of the efficient methods of gene delivery to mammalian cells both in vitro and in vivo. Through years of experience with lentiviral and retroviral vectors, Targatt has developed own proprietary packaging systems and efficient protocols for the rapid generation of pseudoviral particles.

    Targatt offers express Retrovirus packaging service to produce high-quality, high-titer virus particles using your viral construct with turnaround time of 10 days. Save your time, receive ready-to-transduce viral particles.

     
    Features of Targatt’s virus packaging services
    • Production of virus in a state-of-the art BSL-2 facility with robust quality control in accordance with NIH Biosafety Level 2 criteria
    • Flexibility of virus production scales to meet your research needs
    • Accurate Virus titers as determined by qPCR to measure infectious units per ml ( ifus/ml)
     
    Note:
    • Depending on level of titer requested, please provide 10-40 μg of endotoxin-free lentivector or retrovector plasmid DNA
    • All custom virus production services should come with information on the plasmids which have to be packaged.
    • Vector must be able to produce virus (no internal poly A signal, no toxic genes, no unusual 2nd structure, or 5' to 3' LTR size over 8kb.)
    Monday, 11 March 2013 15:51

    Lentivirus Packaging

    Targatt provides high quality and high titer 10-day Lentivirus packing services.

    Lentiviral / retroviral vectors are one of the most efficient methods of gene delivery to mammalian cells both in vitro and in vivo. Through years of experience with lentiviral and retroviral vectors, Targatt has developed own proprietary packaging systems and efficient protocols for the rapid generation of pseudoviral particles.

    1. offers express lentiviral/retrovirus packaging service to produce high-quality, high-titer virus particles using your viral construct with turnaround time of 10 days. Save your time and receive ready-to-transduce viral particles.
     
    Features of Targatt’s virus packaging services
    • Production of virus in a state-of-the art BSL-2 facility with robust quality control in accordance with NIH Biosafety Level 2 criteria
    • Flexibility of virus production scales to meet your research needs
    • Accurate Virus titers as determined by qPCR to measure infectious units per ml ( ifus/ml)
     
    Note:
    • Depending on level of titer requested, please provide 10-40 μg of endotoxin-free lentivector or retrovector plasmid DNA
    • All custom virus production services should come with information on the plasmids which have to be packaged.
    • Vector must be able to produce virus (no internal poly A signal, no toxic genes, no unusual 2nd structure, or 5' to 3' LTR size over 8kb.)
    Monday, 11 March 2013 15:49

    DNA Cloning Service

    With decades of experience our Molecular Biology Team has developed an exceptional expertise in anything DNA-related. We  can help you develop the best strategy to tackle your cloning projects and find solutions to all your technical problems. 

    Some examples of our services:

    • - Cloning of amplified DNA fragments
    • - Gene targeting vectors for homologous recombination
    • - Bacterial Artificial Chromosome (BAC) Recombineering
    • - RNAi and inducible vectors
    • - Site-specific mutagenesis
    • - Tagging of genes
    • - De novo gene synthesis

     

    A typical cloning procedure includes:

    • - Primer design for target region
    • - PCR amplification of target region
    • - Cloning of the PCR product into a cloning vector
    • - Sub-cloning the gene into the vector of your choice
    • - Picking, plating, culturing, and DNA preparation
    • - Sequence confirmation
    • - Plasmid purification (Mini, Midi, or Maxi size)
    Monday, 11 March 2013 15:47

    Stem Cell Culture Services

    We offer standard and customized cell culture services including mycoplasma testing and FBS lot evaluation.

    Please contact us to discuss your needs with our technical service specialists.

    Monday, 11 March 2013 15:44

    Stem Cell Differentiation

    Targatt offers various services for the differentiation of ESC/iPSC lines into more specialized cells including multipotent stem cells and fully differentiated somatic cells.

    Advantage of using multipotent stem cells

    • Thaw ESC/iPSC-derived multipotent stem cells (e.g. Neural Stem Cells) when you need them
    • Differentiate multipotent stem cells to specialized somatic cells (e.g. neurons and glial cells)
    • Expand the multipotent stem cells for a ready supply for your planned experiments = save money and time!

    Standard services:

    • Neural Stem Cells
    • Neural Crest Stem Cells
    • Cardiomyocytes
    • Skeletal Muscle

     

    Bespoke services:

    We also offer customized differentiation services. Please contact us with details about what kind of cells you are interested in.

     

    Human ESC / iPSC Neuronal Differentiation Service

    • Send us your pluripotent cell line (or use our iPSC generation service)
    • We will differentiate your ESC/iPSC line into neural stem cells (NSCs) or neural crest stem cells (NCSC) by using our proprietary neural induction protocol.
    • NSCs and NCSCs are proliferating cells that can be frozen and cultured over a prolonged period of time.
    • NSCs can be further differentiated into multiple neural cell types.

     

    stemcell-targatt-antibodies-gentaur-4

    Monday, 11 March 2013 15:39

    Teratoma Formation Analysis Services

    Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.

    Features:

    • - Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
    • - Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
    • - Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

     

    Service Includes:

    • - Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
    • - Tissue blocks and H&E stained tissue section slides

     

    Notes:

    • - Frozen sample culture 
    • - Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) 
    • - Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request.
    • - Frozen tissue blocks are available.

     

    Technical Details:

    • Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
    • Injection site:Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
    • Service includes:Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.

    teratoma-gentaur-targatt-1

    teratoma-gentaur-targatt-2

    teratoma-gentaur-targatt-3

    Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.

    Features:

    • Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
    • Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
    • Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

    Service Includes:

    • Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
    • Tissue blocks and H&E stained tissue section slides

    Notes:

    • Frozen sample culture Fee $500 per line
    • Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) $200 per line
    • Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request with additional $100 per line.
    • Frozen tissue blocks are available with additional $100.
    Monday, 11 March 2013 15:36

    Stem Cell Characterization

    Human and Mouse ESC/iPSC Characterization in vivo & in vitro

    • Teratoma Formation Analysis Service
    • EB Formation and Analysis Service
    • Pluripotency Marker Characterization (Human / Mouse)
    • Karyotyping (Human / Mouse)
    • Germline Transmission of mouse ESCs
    • Sex determination

    We make the line for you, and we characterize it, too!

    We offer a wide range of services to help you identify and characterize stem cell lines. 

    stemcell-gentaur-targatt-antibodies

    Monday, 11 March 2013 15:33

    Stem Cell Generation

    We have successfully derived many induced pluripotent stem cell (iPSC) lines from patient specific fibroblasts.

    We have three methods for iPSC generation: mRNA, episomal (both genomic footprint-free), or retroviral.

    Genomic footprint-free Induced Pluripotent Stem Cell (IPSC) Generation Service:

    • - No genomic footprint - the non-integrating system is safe in drug discovery and cell therapy applications.
    • - Two methods available: Episomal DNA vectors containing the reprogramming factors or mRNA.
    • - High reprogramming efficiency (0.2%-0.5%).
    • - The reprogramming vectors/mRNA are removed naturally during the cell cycle.
    • - Safe to handle - no viral particles.

     

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    Monday, 11 March 2013 15:30

    Stem Cell Derivation

    Targatt offers a mouse embryonic stem cell (ESC) derivation service from any strain of your interest. We can also derive ESC lines from transgenic mice.

    Monday, 11 March 2013 15:26

    Cell Line Gene Modification

    Cell line gene modification is a versatile genetic tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications.

    Gene targeting is primarily performed in embryonic stem (ES) cells for the purpose of generating knock-out mice. However, targeted gene modification in cultured cell lines can be a considerably easier and more rapid approach of creating valuable research models. Customized gene-targeted mammalian cell lines are a cheap and convenient model system that can be used for many applications in research and drug discovery, including gene function studies and high-throughput screening.

    With strong expertise in genetic modification, gene targeting and cell culture, Targatt can help you to make your customized gene modified cell line. We specialized in both adherent and suspension cell culture. We have many traditional mammalian cell lines in stock and we can also adapt our established targeting techniques to less commonly used cell lines. With a great variety of tools for cell line gene modification in place we have successfully generated gene-targeted clonal lines even from hard-to-transfect cells.

    Available Gene Modification Strategies (selected):

    • Knock-in/knock-out
    • Gene replacement
    • Gene editing
    • Gene therapy
    • Mutagenesis

    Each project is individually devised and realized. Our service includes an initial project assessment based on your model requirements. A dedicated PhD level project manager will discuss available gene targeting methods, vector design strategies and special culture requirements with you. After targeting we will clonally expand and cryopreserve several stable transgenic cell lines. Upon request we can expand the clones further on a large scale for high-throughput assays.

    Applications (selected)

    • - Recombinant protein production in CHO cells
    • - Disease model in human cell lines for drug discovery
    • - Gene therapy in diseased cell line
    • - Generation of a specific cell line with TARGATT docking site as a master cell line for site-specific gene knock-in reporter cell line for gene function studies

     

    Targatt also offers additional services wtihin our cell line models line including TARGATT Gene Insertion.

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