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    Wednesday, 06 February 2013 11:41

    QuantiChrom™ Urea Assay Kit

    Rate this item
    (1 Vote)

    Catalog number : DIUR-500
    Quantity: 500 tests
    Availability: Yes

    DESCRIPTION:
    Urea is primarily produced in the liver and secreted by the kidneys. Urea
    is the major end product of protein catabolism in animals. It is the
    primary vehicle for removal of toxic ammonia from the body. Urea
    determination is very useful for the medical clinician to assess kidney
    function of patients. In general, increased urea levels are associated with
    nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal
    diseases, e.g., congestive heart failure, liver diseases and diabetes.
    Decreased levels indicate acute hepatic insufficiency or may result from
    over-vigorous parenteral fluid therapy.
    Simple, direct and automation-ready procedures for measuring urea
    concentration or blood urea nitrogen BUN in biological samples are
    becoming popular in Research and Drug Discovery. BioAssay Systems'
    urea assay kit is designed to measure urea directly in biological samples
    without any pretreatment. The improved Jung method utilizes a
    chromogenic reagent that forms a colored complex specifically with urea.
    The intensity of the color, measured at 520nm, is directly proportional to
    the urea concentration in the sample. The optimized formulation
    substantially reduces interference by substances in the raw samples.

    KEY FEATURES:
    Sensitive and accurate. Use 5 μL samples. Linear detection range 0.08
    mg/dL (13 μM) to 100 mg/dL (17mM) urea in 96-well plate assay.
    Simple and high-throughput. The procedure involves addition of a single
    working reagent and incubation for 20 min. Can be readily automated as a
    high-throughput assay for thousands of samples per day.
    Improved reagent stability and versatility. The optimized formulation has
    greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
    Low interference in biological samples. No pretreatments are needed.
    Assays can be directly performed on raw biological samples i.e., in the
    presence of lipid and protein.

    APPLICATIONS:
    Direct Assays: urea in serum, plasma, urine, milk, cell/tissue culture,
    bronchoalveolar lavage (BAL) etc.
    Drug Discovery/Pharmacology: effects of drugs on urea metabolism.
    Environment: urea determination in waste water, soil etc.

    KIT CONTENTS: (500 tests in 96-well plates)
    Reagent A: 50 mL Reagent B: 50 mL
    Urea standard: 1 mL 50 mg/dL
    Storage conditions. The kit is shipped at room temperature. Store all
    components at 2-8°C. For long-term storage, keep standard at –20°C.
    Shelf life: 12 months after receipt.

    Precautions: reagents are for research use only. Normal precautions for
    laboratory reagents should be exercised while using the reagents.

     Price: 339 EUR

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