Catalog Number: 9125 (25-50 tests)
Quick Overview
Forget the lysates. Assay for apoptosis via caspase-3 activity in whole, living cells with the FLICA® 660 Caspase-3 Assay Kit.
- Ex: 660 nm / Em: 680-690 nm
- Whole cell analysis via flow cytometry or fluorescence microscopy
- Flexible multiparametric analysis with additional dyes or probes
- Compatibility with GFP and green autofluorescence
- Benchtop flow compatible
This new caspase-3 assay for in vitro apoptosis detection employs a new far-red fluorescent caspase-3 and -7 inhibitor probe, FLICA® 660-DEVD-FMK, to label active caspase-3 and-7 enzymes in living cells or tissue samples. Image the fluorescent signal using fluorescence microscopy or analyze samples for caspase-3 and -7 activity by flow cytometry.
The cell permeant FLICA caspase-3 detection probe, 660-DEVD-FMK, is comprised of a caspase-3-affinity inhibitor peptide sequence (DEVD) and a fluoromethyl ketone (FMK) moiety that enable an irreversible, covalent binding event with active caspase enzymes. This newest FLICA caspase probe is labeled with a far-red fluorescent 660 dye reporter, enabling caspase-3 detection via common fluorescence detection methods.
Apoptosis detected in induced Jurkat cells by labeling active caspases 3&7 with far-red fluorescent FLICA 660 Caspase 3/7 Assay