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Tuesday, 12 November 2013 10:08

SensiFAST™ cDNA Synthesis Kit

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Product description
This kit is the best option for customers wanting to generate cDNA ready for real-time applications. The Kit provides a rapid and very sensitive method for first strand cDNA synthesis for use in real -time PCR studies. The TransAmp™ Buffer provides highly optimized components for efficient reverse transcription and includes a unique blend of anchored oligo dT and random hexamer primers to ensure unbiased 3’ and 5’ coverage for enhanced data accuracy. An extremely efficient reverse transcriptase delivers highly robust first strand synthesis and higher cDNA yields from a wide range of input RNA concentrations. SensiFAST cDNA Synthesis Kit offers enhanced reproducibility, sensitivity and efficiency for exceptional performance in subsequent real-time PCR experiments.

Feature: Benefit: Advantege:
Reproducible Unique reverse transcriptase and new
TransAmp™ buffer generate
consistent first-strand cDNA
Reduces the number of replicates the
customer needs to do, saving space and cost
Broad dynamic
range
Ideal for dilute and low-copy samples Improves assay sensitivity as abundant and rare transcripts can be run against each other
Sensitive Real-Time RT-PCR analysis from as
little as 4pg of starting total RNA
Can be used even when starting material is limited
Unbiased cDNA
synthesis
Complete 5' to 3' RNA sequence
representation
Gives more accurate quantification of genes
Rapid cDNA synthesis and real-time PCR in
less than 60 minutes
Allows higher throughput
Optimized Designed to complement the
SensiFAST SYBR and Probe kits
Can be used directly in a real-time assay without further optimization needed

Positioning
The SensiFAST cDNA Synthesis Kit has been optimized for real-time reactions, the new TransAmp Buffer contains reverse transcriptase enhancers that reduce complex RNA secondary structure, so that efficient reverse
transcription of all RNA samples, regardless of the complexity, can be achieved at 42° C, resulting in unparalleled reproducibility between samples.

Cat. Product Price Order
BIO-65053 SensiFAST cDNA Synthesis Kit, 50 Reactions 262 € Order
BIO-65054 SensiFAST cDNA Synthesis Kit, 250 Reactions 980 € Order

 

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