Gentaur’s AccuPower PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer. AccuPower PreMix PCR master mix includes our super-processive “three times faster than Taq” Top DNA Polymerase for the fastest nucleic acid amplification. While Top DNA Polymerase is ideal for applications where you would normally use Taq, if your needs include HotStart PCR, or High Fidelity PCR, please see our AccuPower HotStart PCR and AccuPower TLA product pages. AccuPower PreMix PCR master mix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer.
Features and Benefits
| Ease of use: | Just add template and primers and start your PCR, dNTPs, buffer and enzyme are provided |
| Stability: | Stable at room temperature for a month and for 2 years in a -20°C freezer |
| Gel loading: | Available with or without a tracking dye for ease of use |
Specifications
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
Terminal transferase activity: Yes
Fragment Size: Up to 10 kb
Application
Standard PCR
Figure 1. Processivity comparison with Lambda DNA template and different PCR reagents.
M: 100 bp Plus DNA Ladder (D-1035)
A: Accupower PCR PreMix
B: Taq mastermix from Company S
C: Taq mastermix from Company T
Lane M: 1 kb DNA Ladder (D-1040)
Lane 1: 1 kb PCR product
Lane 2: 2 kb PCR product
Lane 3: 3 kb PCR product
Lane 4: 4 kb PCR product
Figure 2. Comparison of sensitivity test for AccuPower PreMix PCR master mix and other companies' products using serial diluted human genomic DNA.
Reaction condition: 95°C for 5 minutes, followed by 35 cycles of 20 seconds at 95°C, 20 seconds at 60°C, 30 seconds at 72°C.
A: human Insulin receptor gene
B: DNA cross-link repair 1A gene
I: AccuPower PCR PreMix
II: Taq DNA Polymerase from Company A
III: Taq DNA Polymerase from Company Q
IV: PCR PreMix from Company I
M: 100 bp DNA ladder (D-1030)
Lane 1: 10 ng
Lane 2: 1 ng
Lane 3: 100 ng
Lane 4: 10 ng
