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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    52062 Aachen Deutschland
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    Gentaur London

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    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
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    Kuiper 1 
    5521 DG Eersel Nederland
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    Piazza Giacomo Matteotti, 6, 24122 Bergamo
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    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
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    Friday, 01 March 2013 10:48

    AccuPower HotStart PCR PreMix

    Rate this item
    (1 Vote)

    AccuPower HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart PCR master mix.

    Gentaur uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Gentaur’s Top DNA Polymerase is inhibited at lower temperatures (< 70°C) by pyrophosphate. However, Top DNA Polymerase is rendered fully active at temperatures above 70°C via pyrophosphate hydrolysis with our thermostable pyrophosphatase. This prevents the formation of mis-primed products and primer-dimers during the reaction set up process resulting in improved PCR specificity. It is ideal for nucleic acid amplification reactions involving complex genomic or cDNA templates, very low copy targets, and multiplex reactions.

     

    Features and Benefits

    Ease of use: Just add template and primers and start your PCR, dNTPs, buffer and enzyme are provided
    Stability: Stable at room temperature for a month and for 2 years in a -20°C freezer
    HotStart: Unique enzyme mediated HotStart results in greater specificity and more robust reactions

     


    Specifications

    5' to 3' exonuclease activity: No
    3' to 5' exonuclease activity: No
    Terminal transferase activity: Yes
    Fragment Size: Up to 12 kb


    Application

    HotStart PCR up to 12 kb
    PCR with complex genomic templates/low copy
    Templates cDNA
    Multiplex PCR reactions

    3RocketScript gentaur Cycle RT antibodies PreMix DualStar qPCR gentaur antibodies PreMix DNA

    Figure 1. Specificity comparison between AccuPower PCR PreMix and AccuPower HotStart PCR PreMix. 50 ng human genomic DNA was amplified into 2.5 - 3 kb fragments with the two products.

     

    A: AccuPower PCR PreMix
    B: AccuPower HotStart PCR PreMix
    Lane M: 1 kb DNA Ladder (D-1040)
    Lane 1: 2.5 kb product
    Lane 2: 3.0 kb product

    4PreMix RocketScript gentaur Cycle RT antibodies PreMix

    Figure 2. Specificity comparison of multiplex PCR with various HotStart PCR reagents. Each PCR mixture with 2 ng of human genomic DNA and 2 pairs of primers (p53 + p55 and p55 + p63) was incubated for 2 hours at 37°ree;C prior to performing multiplex PCR.

     

    M: 100 bp DNA Ladder (D-1030)
    A: AccuPower HotStart PCR PreMix
    B: Control – AccuPower PCR PreMix
    C: Company T's antibody mediated HotStart Taq DNA polymerase (0.5 U)
    D: Company A’s antibody mediated HotStart Taq DNA polymerase (1.0 U)

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