Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. 

Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Germany

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    skype chat

    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Friday, 01 March 2013 11:06

    AccuPower TLA PCR PreMix

    Rate this item
    (1 Vote)

    AccuPower TLA PCR PreMix is a high fidelity PCR master mix that contains an easy to resuspend lyophilized mix of TLADNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer conveniently packaged in individual tubes or plates. Simply add template, primers and water. The TLA DNA Polymerase in the premix is a cloned and modified derivative isolated from Thermococcus onnurineus NA1 and features a proofreading activity, an enhanced processivity, and robust amplification efficiency compared to Pfu DNA polymerase or Vent DNA polymerase. These features allow high fidelity DNA amplification as well as long range PCR up to 20 kb.

    AccuPower TLA PreMix high fidelity PCR master mix is available with or without loading dye. When loading dye is present you can load it directly into your gel after PCR – no glycerol or glucose is required.

     

    Features and Benefits

    Ease of use: Just add template and primers and start your PCR, dNTPs, buffer and enzyme are provided
    Stability: Stable at room temperature for a month and for 2 years in a -20°C freezer
    High fidelity: For subcloning and other critical applications



    Specifications

    5' to 3' exonuclease activity: No
    3' to 5' exonuclease activity: Yes
    3’ – A Overhang: No
    Fragment Size: 20 kb


    Application

    Gene synthesis, PCR or primer extension requiring high fidelity, blunt end PCR cloning or mutagenesis, any amplification requiring high fidelity

    5antibodies CycleScript RT gentaur RT-PCR

    Figure 1. Comparison of long PCR amplification between AccuPower TLA PCR PreMix and 2 high-fidelity PCR enzymes. Reaction mixtures were prepared and PCR cycling conditions were performed following the protocol recommendations.

     

    Lane M1: 1 kb DNA Ladder (D-1040)
    Lane M2: Lambda/Hind lll Marker (D-1050)
    Lane 1, 8, 15: 2 kb PCR product
    Lane 2, 9, 16: 5 kb PCR product
    Lane 3, 10, 17: 8 kb PCR product
    Lane 4, 11, 18: 10 kb PCR product
    Lane 5, 12, 19: 12 kb PCR product
    Lane 6, 13, 20: 15 kb PCR product
    Lane 7, 14, 21: 20 kb PCR product

    6power elisa pcr gentaur taq PyroHotStart HotStart

    Figure 2. Comparison of amplification efficiency of AccuPower TLA PCR PreMix and other equivalent products PCR was carried out using AccuPower TLA PCR PreMix and 3 high-fidelity PCR enzymes from other companies. Parallel reactions were performed following the protocol recommendations, using 100 ng, 10 ng, 1 ng and 100 pg human genomic DNA. A 1 kb fragment of the human IR gene was amplified in 30 PCR cycles.

     

    M: 100 bp DNA Ladder (D-1030)
    Lane 1, 6, 11, 16: 100 ng of human genomic DNA
    Lane 2, 7, 12, 17: 10 ng of human genomic DNA
    Lane 3, 8, 13, 18: 1 ng of human genomic DNA
    Lane 4, 9, 14, 19: 100 pg of human genomic DNA
    Lane 5, 10, 15, 10: Negative control template

    7qPCR gentaur antibodies PreMix DNA Ligation PreMix

    Figure 3. . Comparison of detection sensitivity between AccuPower TLA PCR PreMix, Taq DNA polymerase and 2 high-fidelity PCR enzymes in amplification of various sizes. Reaction mixtures were prepared and PCR cycling conditions were performed following the protocol recommendations.

     

    M: 1 kb DNA Ladder (D-1040)
    Lane 1, 6, 11, 16: 2 kb PCR product
    Lane 2, 7, 12, 17: 4 kb PCR product
    Lane 3, 8, 13, 18: 5 kb PCR product
    Lane 4, 9, 14, 19: 7 kb PCR product
    Lane 5, 10, 15, 10: 8 kb PCR product

    8accupower RocketScript RT PreMix accu power elisa

    Figure 4. . A 2 kb Lambda DNA fragment was amplified according to the protocol recommendations using various extension times ranging from 20 sec to 2 min.

     

    M: 1 kb DNA Ladder (D-1040))
    Lane 1, 6, 11, 16: 20 seconds of extension time
    Lane 2, 7, 12, 17: 40 seconds of extension time
    Lane 3, 8, 13, 18: 1 minute of extension time
    Lane 4, 9, 14, 19: 1 minute 30 seconds of extension time
    Lane 5, 10, 15, 10: 2 minutes of extension time

    Order Button1

    Read 1217 times