GENTAUR Europe BVBA
Genprice Inc, Logistics
Serbia, 
Macedonia, 
Montenegro, 
Croatia: 
GENTAUR Romania
GENTAUR Greece 
Above: hESC in ESC-Sure
conditioned medium
Bottom: hESC on MEF TM
Background: Traditionally human embryonic stem cell (hESC) culture requires a mouse embryonic fibroblast (MEF) cells’ support, which makes the protocol complicated. Our Serum- and Feeder-free medium that is for human ESC culture contains all the growth factors needed in hESC culture, except the bFGF; eliminate the routine preparation of feeder. It is a ready-to-use product for a serum/feeder-free system. Our medium makes your cell culture more efficient and easier to control.
Serum- and Feeder-free medium (1x) for proliferation of pluripotent human embryonic stem cell (hESC) culture in a serum/feeder-free system. Each and every batch has been tested for hES/iPS cells pluripotency. All you need to add is 20 ng/ml bFGF.
Product description: 100 mL of hESC-Sure
Source: Chemical plus growth factors
Storage: -80°C
Shipping: Shipped on dry ice.
Recommended procedure:
Human ESC culture procedure using hESC-Sure Serum- and Feeder-free medium:
1. Coat culture dish with Matrigel the day before.
2. Wash Matrigel (BD Biosciences Cat# 354277) coat dishes with DMEM/F12 medium(Targatt Cat# ASM-5002).
3. Once human ES cell were confluent or cultured for more than 5 days, disaggregated cells
with 1mg/ml Dispase(Dilute 5mg/ml stock solution with DMEM/F12 media), incubate at 37
C for 3 minutes.
4. Wash container with 1x PBS, and to scrape colonies off the bottom of the container with cell
scraper.
5. Collect the cells in the 50ml or 15ml falcon tube, pellet by centrifugation at 700rpm for 5 min
at room temperature.
6. Resuspend hESC pellets in hESC-Sure Serum- and Feeder-free culture medium supplemented with 20 ng/ml bFGF.
TM
7. Split cells at 1:3 to 1:5 ratio every 4 to 5 days using 1mg/ml Dispase (diluted with
DMEM/F12).
8. 1x freezing medium contains 90% knockout serum and 10% DMSO.
Price: 104 EUR
