Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.
Features:
- - Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
- - Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
- - Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.
Service Includes:
- - Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
- - Tissue blocks and H&E stained tissue section slides
Notes:
- - Frozen sample culture
- - Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.)
- - Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request.
- - Frozen tissue blocks are available.
Technical Details:
- Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
- Injection site:Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
- Service includes:Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.
Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.
Features:
- Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
- Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
- Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.
Service Includes:
- Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
- Tissue blocks and H&E stained tissue section slides
Notes:
- Frozen sample culture Fee $500 per line
- Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) $200 per line
- Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request with additional $100 per line.
- Frozen tissue blocks are available with additional $100.