- Non-specific sites are virtually eliminated by a polymer coating

- Stable across a wide pH range 2 – 10

- 1000A, 50µm Silica suitable for LC and batch processes

- Applications include: Enzyme immobilization, separation and purification of biopolymers, immobilization of proteins & ligands – monoclonal antibodies, hormones, peptides, haptens, drugs, etc

Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, Silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.

NuGel™ Poly-NHS Technical Data

NuGel™ Poly-NHS is a derivative of NuGel™ polyamino affinity support. This affinity support contains NHS groups at the end of hydrophilic spacer arms and is used to couple ligands containing amino groups.

Download datasheet: NuGel™ Poly-NHS

Product list

Cat.	Product	Quantity	Promo Price	Order
NPHS-25	NuGel™ Poly-NHS	50 mL	470 €

- Hemoglobin voids in flow-through >98%, with <30 minute bind/wash/elute protocol

- Hemoglobin removal from red cell lysates for RBC proteomics

- Hemoglobin removal from hemolyzed serum, blood and dried blood spot/blood card

- Enrichment of hemoglobin variants.

- Low abundance protein and enzyme enrichment

- Disposable, cost-effective and high-throughput

- Mild elution maintains tertiary structure and simple transfer to secondary analysis

- Removes hemoglobin from species including human, sheep, bovine, goat, etc.

- Removes hemoglobin from organs, tissues.

- The eluted fractions retain their enzymatic and biological activity

- The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

HemoVoid™ , a silica-based protein enrichment matrix, removes hemoglobin from erythrocyte lysate samples while concentrating low abundance, and/or low molecular weight proteins. The HemoVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions.

HemoVoid™ derives from a silica-based library of individual mixed-mode ligand combinations (ionic, hydrophobic, aromatic, polymer). The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. HemoVoid™ depletes hemoglobin from red cell lysates while improving the resolution of less abundant blood proteins.

 Download datasheet: HemoVoid™ - Hemoglobin Depletion From Erythrocytes

Product list:

Cat.	Product	Quantity	Promo Price	Order
HVK-05	HemoVoid™, Hemoglobin Depletion	5 mL	291 €
HVK-10	HemoVoid™, Hemoglobin Depletion	10 mL	382 €
HVK-50	HemoVoid™, Hemoglobin Depletion	50 mL	1300 €
HVK-100	HemoVoid™, Hemoglobin Depletion	100 mL	2029 €

- Has a high degree of specificity for hemoglobin binding up to 10 mg/ml

- Applications in blood substitutes, enzyme recovery and analytical interferences

- Removes hemoglobin from any species including human, sheep, bovine, goat, etc

- Removes hemoglobin from organs, tissues.

- Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery

- The flow through fractions retain their enzymatic and biological activity

- The flow through fractions is compatible with LC-MS, activity based protein profiling and proteomic studies.

HemogloBind™ comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. HemogloBind™ is engineered for a high degree of selectivity and does not cross react with most common serum components, making it an excellent tool in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin analysis and bulk serum clarification. Hemoglobin variants, as in thalassemia, bind with differential affinity towards HemogloBind™. For purification and/or analysis of hemoglobin, a modest elevation in pH will facilitate desorption of hemoglobin bound to HemogloBind™

Download datasheet: HemogloBind™ Hemoglobin Depletion From Hemolyzed Serum/Plasma

Product list:

Cat.	Product	Quantity	Promo Price	Order
H0145-05	HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma	5 mL	278 €
H0145-15	HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma	15 mL	400 €
H0145-50	HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma	50 mL	773 €
H0145-15-60P	HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma	15 mL (60 Aliquots of 250 ul HemogloBind each)	616 €
H0145-50-200P	HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma	50 mL (200 Aliquots of 250 ul HemogloBind each)	1188 €

INTERNATIONAL YEAR OF CRYSTALLOGRAPHY - 100 years since Nobel Prize for X–ray diffraction

In 1914, the Nobel Prize in Physics was awarded to Max von Laue for making the connection between X–ray diffraction angles and the size and orientation of spacing between units in a crystal. In 2014, the United Nations General Assembly adopted a resolution proclaiming this year as the International Year of Crystallography. UNESCO and the International Union of Crystallography have been invited to facilitate this proclamation and have put together a year–long program.

CRYO SHUTTER - Annealing is a promising technique to improve poorly diffracting crystals

Cooling your sample to cryo–conditions helps protect the structure of a crystal from radiation and thermal motion. Poor cryo–cooling can increase the sample's mosaicity, leading to poor diffraction data. A brief interruption of the cryo–stream can warm then re–cool the crystal and potentially reduce mosaicity.
The Cryo Shutter, developed by Dr. Uwe Mueller, MX-Lab at BESSY-II, HZB Berlin-Adlershoto is an automated system for crystal annealing, ready for installation on Cryojet (Oxford Instruments) and Cryostream 700 Systems (Oxford Cryosystems).

Features:
• Precise interruption of the cryostream
• Reproducible crystal annealing
• Extremely fast closing and opening of the shutter prevents turbulences

Order: IN SITU PLATE

IN SITU PLATE - The revolutionary 96-well SBS format crystallization plate like no other

The plate's design allows you to:
• Use your current robot to populate
• Setup sitting or hanging drops with the robot
• You can choose from 1 to 6 growth drops per well
• Use from 50 nl - 2 µl size drops
• Obtain more hits and reproducible results
• Use X-rays to screen crystals for diffraction before harvesting
• Get better results from UV screening
• Transport your crystals safely to the synchrotron

Order: IN SITU PLATE

THE MICRORT™ SYSTEM - Easily screen crystals at room–temperature before cryoprotecting and freezing

Room–temperature screening is especially important in protein crystallography, as many low–temperature data sets do not yield diffraction data of sufficient quality to determine a structure. MiTeGen’s patented MicroRT™ system allows you to screen your samples for improper crystal alignment due to damage or poor as–grown crystal quality before you head to the beamline. Introducing routine room-temperature screening of your crystals can easily save your experiment time, money, and effort.

Included:
• 1 MicroRT™ Tubing Kit (20 1.5” (37 mm) long clear polyester capillaries sealed at one end; Extra–keen razor blades for cutting tubes to desired length; Small tube of grease for lubricating the base)
• 1 MicroRT™ Aligner for properly setting the capillary tube over the crystal and onto the base
• 1 box of 20 Dual-Thickness MicroMounts™
• 1 pair of serrated-end tweezers

Features:
• Allows rapid screening of crystals at room temperature, to help maximize the efficiency of your crystallography pipeline.
• Easy sample mounting with little risk of crystal loss or damage.
• Easy collection of both room and low-temperature data from the same sample.
• Easy sample visualization and alignment, especially for small crystals.
• Capillary tubing is easily cut and sealed, and gives significantly less background X-ray scatter than glass capillaries.
• One size capillary fits all, and capillaries are reusable, lowering your cost per measurement.

Order: THE MICRORT SYSTEM

Read more about Crystallography innovation

More: IN SITU PLATEs