-    Semi liquid Calibrators and Controls 

        o   Stability of reconstituted Calibrators and Controls up to 8 weeks at 2-8° or 4 months at -20°

     -     24 months Shelf life

     -     Breakable wells

     -     Optical Density <3.0

The 25-Hydroxyvitamin D Total ELISA is powered by unique monoclonal antibodies that express 100% reactivity against 25 OH Vitamin D3 and 83% reactivity against 25 OH Vitamin D2. All in one technology. No extraction step, displacement solution directly into the well. Results in less than 4 hours. Calibrated against ID-LC/MS-MS (reference method). Bar codes an all components.

Price for this unique Assay, CE and FDA approved:

More: Vitamin D Total ELISA

The GENTAUR Bovine living cysticercosis ELISA is based on 2 mouse monoclonal antibodies that detect only protein of living Cystocerkosis infections. The ELISA can detect Cystocercosis from as low as 10 infections in one adult cattle on 500 ul of crude serum.

The results on Urine are not 100% accurate due to false positives.

Name: Bovine living cysticercosis ELISA

Product reference: 04-bov-cysto-ELISA

Price: 455 Euro / 96 wells

Use: Low detection of living infections, not quantitative but qualitative

Deze ELISA geeft een juiste diagnose in  100% van alle positieve gevallen, wanneer er meer dan 10 cysticercen aanwezig zijn in het karkas( keuringsverslag) Indien er tussen de 1 en 10 cysticercen aanwezig zijn daalt dit naar 65%. In theorie is 1 cysticerc voldoende om een mens te infecteren.

Name:  Rabbit polyclonal anti living bovine cystocercosis

Product reference: 04-bov-cysto-rab500

Price: 195 Euro / 500ul

Use: crude rabbit serum used for WB 1/500, ELISA capture antibody

Bovine cystocercosis is a parasitic disease that afflicts the muscles of cattle and is caused by larvae of the human tapewormTaenia Saginata. If people consume beef containing these parasites they can acquire intestinal tapeworm infections.

Detectietest ter confirmatie van de visuele postmortem diagnose van rundercysticercose

IWT project 080132

Advanced optical system

1. 8 channel optical fiber system enables 5 secs' reading for 96 well plate
2. Auto position function, ensures accurate results
3. Bichromatic measurement, Multi-calculation methods include ABS, Cut-off , Linearity and Log.
4. 405nm, 450nm, 492nm, 630nm, 4 free positions

Intelligent software

1. Big LCD, question and answer mode for program, humanity design, easy to operate
2. PC controlled, Elisa WorkStation Function
3. Self-test enables normal condition for any operate
4. Plate shaking modes editable, enables excellent mixing, giving the reliable results
5. Multi-test Methods, qualitative and Quantitative Test
6. Critical value analysis, quantitative analysis, quality control etc
7. Large storage capacity for 28 test programs
8. Intelligent Operating software, able to communicate with PC

Specifications

1. Plate Type: 96 or 48 well plate or strips
2. Reading Speed: 5 second for 96 well plate
3. Light Source: Cold light source
4. Wavelength Range: 340~750nm
5. Filters: 405nm, 450nm, 492nm, 630nm, 4 open positions
6. Linearity range: ±1%(0.000-2.000Abs)
7. Measure Mode: Monochromatic , Bichromatic
8. Plate shaking : Time &Speed adjustable
9. Display: 240*128LCD
10. Interface: Bi-directional LIS interface for data transfer and handling
11. Power supply: AC220V/110V±10%,50/60Hz
12. Dimension: 440(L)mmX305(W)mmX154(H)mm
13. Net weight: 7kg

Price: 1400 Euro

More: Elisa Microplate Reader

Recombinant Aedes aegypti 37 kDa salivary gland allergen Aed a 2(D7) for Aedes aegypti (Yellowfever mosquito) (Culex aegypti)

CSB-YP321587AXQ >> Yeast € 2090/ mg
CSB-EP321587AXQ >> E.coli € 1680/ mg
CSB-BP321587AXQ >> Baculovirus € 2540/200ug
CSB-MP321587AXQ >> Mammalian cell € 2980/200ug
Expression Region ：18-321aa；full length.

INTENDED USE
The CA15-3 for the quantitative determination of the Cancer Antigen CA15-3 concentration in human serum. This kit is intended for research use only.

SUMMARY AND EXPLANATION
Breast cancer is the most common life-threatening malignant lesion in women of many developed countries today, with approximately 180,000 new cases diagnosed every year. Roughly half of these newly diagnosed patients are node-negative, however 30% of these cases progress to metastatic disease.
There are a number of tumor markers that can help clinicians to identify and diagnose which breast cancer patients will have aggressive disease and which will have an indolent course. These markers include estrogen and progesterone receptors, DNA ploidy and percent-S phase profile, epidermal growth factor receptor, HER-2/neu oncogene, p53 tumor suppressor gene, cathepsin D, proliferation markers and CA15-3. CA15-3 is most useful for monitoring patients post-operatively for recurrence, particularly metastatic
diseases. 96% of patients with local and systemic recurrence have elevated CA15-3, which can be used to predict recurrence earlier than radiological and clinical criteria. A 25% increase in the serum CA15-3 is associated with progression of carcinoma. A 50% decrease in serum CA15-3 is associated with response to treatment. CA15-3 is more sensitive than CEA in early detection of breast cancer recurrence. In combination with CA125, CA15-3 has been shown to be useful in early detection of relapse of ovarian
cancer. CA15-3 levels are also increased in colon, lung and hepatic tumors.

PRINCIPLE OF THE TEST
The CA15-3 ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA15-3 molecule is used for solid phase immobilization (on the microtiter wells). A rabbit anti-CA15-3 antibody conjugated to horseradish peroxidase (HRPO) is in the antibody-enzyme conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the CA15-3 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate 1-hour incubation steps at 37C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of CA15-3 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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Antigen: Tenascin C (TNC)

Synonyms: TN, HXB, MGC167029, Hxb, Ten, TN-C, AI528729, MGC144208, MGC144209, cytotactin, tenascin-C, C130033P17Rik, tenc, wu:fk04d02, TNC, tn, MGC140517

Quantity: 96 Tests/kit

Components:

1. Assay plate (12 x 8 coated Microwells). Quantity: 1(96 wells)
2. Standard (Freeze dried). Quantity: 2
3. Biotin-antibody (100 x concentrate) Quantity: 1 x 120 µl
3.HRP-avidin (100 x concentrate). Quantity: 1 x 120 µl
4. Biotin-antibody Diluent. Quantity: 1 x 10 ml
5. HRP-avidin Diluent. Quantity: 1 x 10 ml
6. Sample Diluent. Quantity: 1 x 20 ml
7. Wash Buffer (25 x concentrate). Quantity: 1 x 20 ml
8. TMB Substrate. Quantity: 1 x 10 ml
9. Stop Solution. Quantity: 1 x 10 ml
10. Adhesive Strip (For 96 wells). Quantity: 4
11. Instruction manual

Description Synonyms: CMD1Z, TNC, TNNC, cardiac troponin C|slow twitch skeletal/cardiac muscle troponin C|troponin C, slow|troponin C1, slow

Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Minimum Detection Limit: 0.195 ng/mL

Detection Range: 0.78 ng/mL - 50 ng/mL

Assay Precision: 
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Price: 1087 EUR

The Gentaur 25OH Vitamin D Total ELISA is a solid phase Enzyme Linked Immunosorbent Assay performed on microtiterplates. During a first 2 hours incubation step, at room temperature, total 25OH Vitamin D (D2 and D3) present in calibrators, controls and samples is dissociated from binding serum proteins to fix on binding sites of a specific monoclonal antibody. After 1 washing step, a fixed amount of 25OH Vitamin D-labelled with biotin in presence of horseradish peroxidise (HRP), compete with unlabelled 25OH Vitamin D2 and 25OH Vitamin D3 present on the binding sites of the specific monoclonal antibody. After a 30 minutes incubation at room temperature, the microtiterplate is washed to stop the competition reaction. The Chromogenic solution (TMB) is added and incubated for 15 minutes. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is inversely proportional to the total 25OH Vitamin D (D2 and D3) concentration. A calibration curve is plotted and the total 25OH Vitamin D (D2 and D3) concentrations of the samples are determined by dose interpolation from the calibration curve.

Price: 250 €

Technical spec.:

Download "Instructions for Use": KAP1971.pdf

INTENDED USE

The IMMUNO-TEK Rat IgG ELISA* Kit is a rapid, easy to use enzyme linked immunosorbent assay (ELISA) designed for the measurement of rat IgG in rat serum, plasma, saliva, mucosa, cell culture fluid from rat cells or other biological sample. The assay contains ready-to-use reagents and takes less than two hours to perform. The microplate and detector antibody in the kit react with all subclasses of rat IgG.

* Research Purposes Only.  Not For in vitro Diagnostic Use.

PRINCIPLE OF THE TEST

Microplate wells coated with polyclonal antibodies to rat IgG form the capture phase of the assay. Rat IgG specimen samples along with the supplied standard are diluted appropriately with Assay Diluent and are then incubated in the microplate wells. After a wash step, captured rat IgG in the well is incubated with detector antibody, a polyclonal anti-rat IgG conjugated to horseradish peroxidase (HRP). This antibody reacts only to the Fc portion of the immunoglobulin molecule allowing for the specific detection of IgG. After another wash step, the chromogenic substrate tetramethyl benzidine (TMB) is added and a blue color develops in proportion to the amount of rat IgG that has been bound to the antibody-coated plate. The enzyme reaction is stopped by the addition of Stop Solution which results in a color change to yellow which can be measured spectrophotometrically at 450 nm. The concentrations of rat IgG are then calculated from a standard curve.

Download Rat IgG ELISA Datasheet

Download SeroDetect West Nile Virus Panel

The Cysticercosis Antigen (Ag) ELISA (Ref. 650501) is a sandwich Enzyme-Linked ImmunoSorbent Assay based on monoclonal antibodies for the qualitative determination of viable metacestodes (cysticerci) of Taenia spp. in human and porcine serum samples.

-  Analytical sensitivity: 1 cyst is detectable in certain conditions
- Incubation times: assay 45 minutes + sample prep <30 minutes
- Available format: 96T

Taenia solium cysticercosis is an infection of humans and pigs with metacestode larvae (cysticercus) of Taenia solium. Circulating antigen detection in serum is an important diagnostic method that indicates the presence of viable parasites. The monoclonal antibodies used in this assay are produced against excretory secretory products (ESP) of viable T. saginata cysticerci. The glycoprotein antigens detected by these monoclonal antibodies are present on the tegument and in the excretory secretory products of metacestodes.

The assay demonstrates the presence of viable cysticerci only, it does not detect degenerated or calcified cysticerci. In this respect, unlike antibody detection, measurement of circulating antigen levels allows differentiation of cysticercosis cases with viable parasites, with antigen levels correlating to the numbers and size of lesions. It can as such also provide a tool for serological monitoring of antiparasitic therapy in human or pigs: antigen levels drop rapidly after successful anthelminthic treatment.

Porcine cysticercosis

The assay is genus specific, not species specific. The assay does not allow the differentiation between infections of different Taenia species in pigs. In experimentally infected pigs, circulating antigens were first detected between 2 and 6 weeks post infection and remained present generally throughout an observation period of 6 months, even in pigs carrying only five to eight living cysts. The minimum number of living cysts, that could be detected using the Cysticercosis Ag ELISA, was one.

Human cysticercosis

Because T. solium is the only Taenia sp. causing cysticercosis in man, the test is specific. No cross-reactions were observed with sera from patients with other parasitologically and/or serologically confirmed infections. The sensitivity of the assay decreases when the number of viable cysts is low; infections with one viable cyst are often not detectable. Antigen levels are generally higher in extraparenchymal neurocysticercosis (NCC) (particularly subarachnoid NCC) than in intraparenchymal NCC; therefore, high antigen levels should lead one to suspect the presence of extraparenchymal NCC.

Download datasheet