Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

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    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

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    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

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    Friday, 01 March 2013 13:45

    AccuPower DNA Ligation PreMix

    18AccuPowerDNALigationantibodies PreMix DualStar qPCR

    The AccuPower DNA Ligation PreMix is a lyophilized master mix containing T4 DNA Ligase, ATP, reaction buffer, and patented stabilizer. This DNA ligation premix is conveniently aliquoted in strip-tubes for reactions; you need only add DNAs to be ligated and water. The reaction will work for DNA ligation for all applications: blunt cloning, sticky end cloning and TA cloning. The premix is stable up to four months at room temperature and for three years at -20°C.

     

    Features and Benefits

    Ready to use premix: Minimal set up time and handling required
    Fast: Only 5 minutes for cohesive-end ligation and 10 minutes for blunt-end ligation at room temperature
    Stable: Enzyme activity for up to four months at room temperature and for three years in the freezer



    Application

    Cloning into vectors, library construction, TA cloning, linker ligation, and re-circlization of linear DNA

    19taq PyroHotStart HotStart

    Figure 1. Stability test of AccuPower DNA Ligation PreMix at room temperature.

     

    Lane 1 – 12: Lambda DNA / Hind lll fragment (1 µg)
    Lane 12 – 24: Lambda DNA / EcoR V fragment (1 µg)
    Lane 2, 3, 14, 15: Ligation with AccuPower Ligation PreMix stored at room temperature for 1 month
    Lane 5, 6, 17, 18: Ligation with AccuPower Ligation PreMix stored at room temperature for 2 months
    Lane 8, 9, 20, 21: Ligation with AccuPower Ligation PreMix stored at room temperature for 3 months
    Lane 11, 12, 23, 24: Ligation with AccuPower Ligation PreMix stored at room temperature for 4 months

    20pcr gentaur taq PyroHotStart HotStart

    Figure 2. DNA Ligation efficiency comparison between AccuPower DNA Ligation PreMix and other competitors’ products.

     

    Lane 1, 9: Intact Lambda DNA (1 µg)
    Lane 2 – 8: Lambda DNA / Hind lll fragment (1 µg)
    Lane 10 – 16: Lambda DNA / EcoR V fragment (1 µg)
    Lane 3, 4, 11, 12: Ligation with AccuPower Ligation PreMix
    Lane 5, 13: Ligation with T4 DNA Ligase from company N
    Lane 6, 14: Ligation with Quick Ligation Kit from company N
    Lane 7, 15: Ligation with LigFast Rapid DNA Ligation System from company P
    Lane 8, 16: Ligation with Ready-To-Go T4 DNA Ligase from company A

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    Published in Promos