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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

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    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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    52062 Aachen Deutschland
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
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    547, Yurok Circle
    San Jose, CA 95123
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    (408) 780-0908 

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    San Jose, CA. 96123

     

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    Tel 00302111768494 
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    Other countries

    Other countries
    Luxembourg +35220880274
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    Caspase-3 Assay for Apoptosis Detection in Whole, Living Cells 
    Catalog no. 9125 (25-50 tests)

    flica-660-caspase-3-assay-apoptosis-positive-jurkat-cellsApoptosis is an evolutionarily conserved form of cell suicide mediated by a cascade of proteolytic enzymes called caspases. Pro-apoptotic signals activate the enzymatic cascade resulting in the cleavage of protein substrates, leading to the disassembly of the cell. Caspases have been identified in organisms ranging fromC. eleganstoMembers of the mammalian caspase family of cysteinyl aspartate-specific proteases play distinct roles in apoptosis and inflammation.

    Caspases
    Active caspase enzymes exhibit catalytic and substrate specificities comprised of short tetra-peptide amino acid sequences that must contain an aspartate in the P1 position. These preferred tetra-peptide sequences have been used to derive peptides that specifically compete for caspase binding. In addition to the distinctive aspartate cleavage site at P1, the catalytic domains of the caspases typically require four amino acids to the left of the cleavage site with P4 as the prominent specificity-determining residue. Addition of a fluoromethyl ketone (FMK) to the tetrapeptide results in an irreversible linkage and permanent inactivation of the cysteine protease enzyme. Furthermore, conjugation of a fluorescent moiety at the amino terminus yields a probe that allows for the detection of caspase activity.

    FLICA® 660 Caspase-3 Assay: Detection Mechanism

    The far-red FLICA® 660-DEVD-FMK caspase-3 detection probe is comprised of the preferred affinity peptide sequence (DEVD) targeted by activated caspase-3 and caspase-7, a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting fluorescent caspase-3 inhibitor probe forms an irreversible, covalent bond with active caspase-3 enzymes, efficiently labeling the target for detection. Due to its cell permeant nature and fluorescence properties, the far-red FLICA caspase-3 detection probe enables whole cell analysis via common fluorescence detection methods.

    To use FLICA Caspase-3 Assay, add the caspase-3 detection probe directly to suspension cell or tissue culture media, incubate, and wash. The cell permeant, far-red FLICA caspase-3 detection probe will efficiently diffuse into cells and irreversibly bind to activated caspase-3 enzymes, thereby retaining the red signal inside caspase-3-positive cells. Cells not bearing active caspase-3 return to a non-fluorescent status after the wash step.

    The FLICA® 660 caspase-3 detection probe has an optimal excitation at 660 nm and optimal emission range from 680-690 nm. As such, it has demonstrated excellent excitation efficiency with a conventional red HeNe laser with a 633 nm excitation, enabling samples to be analyzed with most flow cytometers and fluorescence microscopes equipped with electronic grey scale image capabilities. Cells labeled with the FLICA® caspase-3 detection probe may be read immediately or preserved for 16 hours using the fixative.

    Cat #: 9125 (25-50 tests)

    Price: 250 Euro (without VAT)

     

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    Published in Promos


    A MUST FOR:

    • - Mouse-On-Mouse
    • - Human and Animal IHC
    • - Immunofluorescence
    • - in-situ Hybridization


    background busterBackground Buster is highly effective for quenching background fluorescence and is easy to use: 

    For Fluorescence:

    • Apply Background Buster for 15 minutes prior to application of the first antibody (unlabeled or fluorescence-labeled antibody).
    • Rinse with PBS for 1 minute.
    • Apply unlabeled antibody for indirect method or fluorescence labeled antibody for direct method and continue with usual protocol.

     

    Background Buster can be used for IHC:

     background buster ihc

     

    Background or non-specific staining is often observed in a variety of immunoassays, in immunohistochemistry and other immunoassay types such as immunofluorescence, ELISA and flow cytometric assays, background staining can be prevented by the use of INNOVEX Recombinant Protein Technology,Background Buster.


    Background Buster is also applicable to eliminating non-specific binding in immunofluorescence, ELISA and flow cytometric assays.


    ADVANCED & UNIQUE FEATURES:

    • Allows staining of identical species antibodies and tissues (e.g., mouse antibody on mouse tissue, rat-on-rat, rabbit-on-rabbit, etc.).
    • Short 10 minute incubation step prior to applying primary antibody or in-situ probe at room temperature
    • Delivers complete eradication of general background staining
    • Replaces the use of normal serum, powdered milk, casein, and other blocking agents and renders complete success
    • Excellent for both frozen and paraffin sections
    • Excellent for in situ application
    • A must for animal tissue staining

    Download datasheet PDF file

    Published in Promos