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Several teams scientists experimenting with dangerous strains of avian flu
These laboratory studies have opponents, there is no guarantee that nature will follow the theoretical approach.
While some scholars argue that it should be kept for research purposes genetically manipulated viruses of avian influenza in several laboratories around the world are already conducting similar experiments, reported the German magazine "Focus". Several teams working in parallel with genetically manipulated virus H7N9. Ron Fucino, one of the scientists involved in the experiments confirmed that his colleagues in several places around the world already are testing resistance to drugs.
Other attempts were allegedly in the pipeline, says Fucino who specializes Flu Medical Center "Erasmus" in Rotterdam. They will study the behavior of the virus modified so as to be easily transmitted from person to person. From mid-March in China already has at least 130 people infected with the dangerous strain H7N9.
This week leading experts called in the journal "Nature" to be carried out similar experiments in which the causative agent is modified in vitro so as to be more infectious and resistant to treatment with drugs. They hope that science will overtake both real life and will be prepared to respond to any mutations in the strain of bird flu, which has threatened a number of Asian countries.
Such an approach, however, meeting and much criticism. According to the China Center for combating infectious diseases in natural mutation of a virus can take years. In the laboratory, it happened in an instant. There is no guarantee that the actual mutations will follow artificial scientific model.
According to Ron Fucino however scientific experiments are very useful and are based on actual forecasts. Attempts would make it possible to produce more effective vaccines and scientific teams would predict risks of transmission of the virus from person to person. New form of H7N9 bird flu was confirmed in humans for the first time in March 2013 Health officials believe that spillover occurred from birds to humans. Thousands of birds were killed and live poultry markets were closed. An outcome measure, the number of new infected dropped almost to zero. In April, the WHO issued a warning that in some cases the virus can be passed from person to person. Scientists from China have joined this opinion and wrote in the British Medical Journal, the risk of bird flu is by no means
Scientists: New bird flu is transmitted from person to person
Deadly H7N9 occurs for the first time in March
Chinese scientists have found the most convincing evidence so far that the deadly new strain of bird flu, "jump" from person to person. Experts emphasize, however, that the virus still can not be easily transmitted between humans, BTA reported.
The first scientific analysis of the transmission of the strain H7N9, showed for the first time in China in March this year, is published in the journal "British Medical Journal".
The study analyzed the case of a father and daughter from East China died after infection with H7N9. Experts have not been able to talk to them since they were both in serious condition, but questioned the family members and loved ones
friends. The analysis has shown that it is highly likely that the virus will be transmitted directly from the 60-year-old man, his daughter.
In a commentary accompanying the publication, experts note that "the study does not necessarily mean that H7N9 will be the next culprit an influenza pandemic, but stresses the need for extreme vigilance."
"The threat, which is H7N9, does not over," commented James Radzh and Richard Coker of the London School of Hygiene and Tropical Medicine.
The authors of this study from the Centre for prevention and control of diseases in Jiangsu province, however, stressed that the new strain has not yet acquired the ability to transmit efficiently from person to person. This means that the risk of the virus in its current form can cause human pandemic is very low.
The new H7N9 strain of bird flu occurs in March in China. According to WHO, the virus has so far infected 133 people in the country and Taiwan, killing 43 of them. Most cases of infection among people who have visited markets for live birds or had close contact with live birds between seven and 10 days to get sick.
The study, published in the journal "British Medical Journal" analyzed "family" case of two patients - a father and daughter from East China. 60-year-old man regularly visited the market for live poultry and got sick between five and six days after the last contact with birds. He was admitted to hospital on March 11. Following the deterioration of symptoms the patient was transferred to the intensive ward on March 15. He died of multiple organ failure on May 4.
The second patient - his healthy 32-year-old daughter, not having visited markets for poultry, but has been in direct contact with his father, caring for him in the hospital before his transfer to the ICU. The woman developed symptoms of infection
six days after the last contact with his father. Admitted to the hospital on March 24, moved in ICU on March 28 and died of multiple organ failure on April 24.
Strains of the virus isolated from samples of both patients were "almost genetically identical" - convincing evidence that H7N9 has passed directly from father to daughter.
"To our knowledge, this is the first report of a possible transmission of the new virus from person to person, accompanied by detailed epidemiological, clinical and virological data," wrote the authors of the study published in the journal "British Medical Journal".
Avian influenza H7N9 Promo
Dear Clients,
We are happy to announce that another promo is available as of today:
1. Intended Use
Avian influenza virus H7N9 real time RT-PCR kit is used for the detection of gene H7 and gene N9 of avian influenza A subtype H7N9 in human nasal and pharyngeal secretions and bird fece by using real time PCR systems.
2. Principle of Real-Time PCR
The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities during Real Time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.
3. Product Description
Highly pathogenic avian influenza (HPAI) caused by certain subtypes of influenza A virus in animal populations, particularly chickens, poses a continuing global human public health risk. Direct human infection by an avian influenza A (H5N1) virus was first recognized during the 1997 outbreak in Hong Kong. The avian influenza virus H7N9 is one subgroup among the larger group of H7 viruses. Some cases of human infection with H7N9 virus in China are confirmed till early April of 2013.
Avian influenza virus H7N9 real time RT-PCR kit contains a specific ready-to-use system for the detection of avian influenza virus H7N9 by Reverse Transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The master contains Super Mix for the specific amplification of the avian influenza virus H7N9 RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the avian influenza virus H9 RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by polymerase chain reaction. Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The detection of amplified avian influenza virus H7N9 DNA fragment is performed in fluorimeter channel FAM and HEX/VIC/JOE with the fluorescent quencher BHQ1. In addition, the kit contains a system to identify possible PCR inhibition by measuring the FAM fluorescence of the internal control (IC).
4. Kit Contents
Ref. |
Type of reagent |
Presentation 25rxns |
1 2 3 4 |
H7N9 Super Mix RT-PCR Enzyme Mix Molecular Grade Water H7N9 Positive Control |
1 vial, 480ml 1 vial, 28ml 1 vial, 400μl 1 vial, 30μl |
Analysis sensitivity: 1×103copies/ml;
Note: Analysis sensitivity depends on the sample volume, elution volume, nucleic acid extraction methods and other factors .If you use the RNA extraction kits recommended, the analysis sensitivity is the same as it declares. However, when the sample volume is dozens or even hundreds of times greater than elution volume by some concentrating method, it can be much.
Our Price: EUR 562
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