Description:

OMNIA LH is a fully automated workstation that allows handling of different fluids, dispensing of various components, homogeneous mixing of even small volumes, multiwell plate preparation and replication, biobanking.

1. easy programming, OMNIA LH can carry out a wide range of protocols accurately and quickly: PCR amplification, real-time qPCR, reverse transcription, methylation, library preparation for next-generation sequencing.

Genomics

• DNA-RNA extraction
• PCR setup
• Real-time qPCR setup
• Reverse transcription setup
• Sequencing setup
• Agarose gel electrophoresis
• DNA purification

Sample preparation

• Plate preparation
• Plate replication
• Autosampling
• Biobanking

Proteomics

Immunology

Main features

• Extreme flexibility of use
• User-friendly graphic interface
• Quick programming of every applicable protocol
• Easy protocol selection from libraries
• Monitoring of activities, also remotely
• Sample traceability
• Customized configuration
• Modular design
• Additional devices available

Manufacturer: MASMEC

Gene synthesis is the most cost effective way to enhance your research. In as little as 2 weeks, you can have your Gene cloned in hand and 100% sequence guaranteed. And with Gentaur’s great pricing it can cost less to order a synthetic gene from us than to buy all the kits and reagents necessary to PCR, ligate, clone, grow, purify and sequence your gene of interest. If you like, our free codon optimization will increase protein expression rates and can enhance protein function. In addition our optimization can make previously un-clonable sections of DNA easy to work with. Send us your sequence, Gene ID or an accession number. Let us know what you need and we will deliver!

Gentaur also offers mutagenesis as well as cloning and subcloning services. Gentaur strives to provide the highest quality synthetic genes at a great price. Our goal is to always provide you with the best value for your research dollar.

With high throughput DNA synthesis facilities around the world, Gentaur’s daily capacity is unsurpassed. Gentaur is unrivalled in its ability to address the needs of our customers: Whether you need one gene, or one hundred. We respond to your needs–personally.

Features and Benefits

100% Sequence Guarantee:	Individual synthetic genes are confirmed by Sequencing
Codon Optimization - Free of charge!:	Complimentary codon optimization to enhance protein expression and function
Value Pricing:	The best value for your research dollar

Applications

Antibody Construction
Antibodies targeted toward specific diseases or targets can be codon optimized for maximum expression in the host organism. Also, an antibody library can be constructed to screen for the most efficient antibody variant.

Organism Production Optimization 
Optimize expression of genes related to resource production to maximize industrial biological production efficiency.

Gene Construction
Get difficult-to-clone DNA sequences easily and enhance the quality of your research by constructing hypothetical genes.

Protein Modification
Codon optimization can increase protein expression efficiency, and a mutant library derived from this process can yield proteins with increased function. Optimizations include secondary structure removal, and repeat reduction as well as organism optimizations.

Schematics of Gene Synthesis Process

Price:  0.25 euro/base

The polymerase chain reaction (PCR) is a common technique used to amplify, or copy, pieces of DNA. Amplified DNA is then used in genetic analyses for everything from medicine to forensics. In plant research, PCR is a vital step in detecting and sequencing genes, and its applications are endless. However, compounds found in plants often inhibit PCR. Researchers at the University of Southern Mississippi discovered that the use of an additive allows PCR to successfully amplify DNA from once problematic plants.

PCR is widely used in plant sciences but is not 100 percent reliable. Many plant researchers encounter roadblocks when implementing PCR. For example, many plant species contain phenolic compounds that deter herbivores. These compounds are often extracted along with plant DNA and can stop PCR from working.

Graduate student Tharangamala Samarakoon and colleagues have found a technique to overcome many of these inhibitory plant compounds. They added a reagent to the PCR mixture that contains three ingredients: trehalose, bovine serum albumin, and polysorbate-20 (all three abbreviated TBT-PAR). "Unlike several other studies, TBT-PAR works at the PCR stage instead of at the DNA extraction stage, so it has promise for pigeon-holed and half-forgotten extractions that previously failed to be amplified using PCR," says Samarakoon. The authors published their research in the January issue of Applications in Plant Sciences.

Samarakoon tested the TBT-PAR reagent on DNA extracted from tropical and temperate species across four plant families, including Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae. PCR with TBT-PAR successfully amplified DNA for all species, whereas standard DNA extraction and PCR techniques consistently failed.

TBT-PAR enhanced PCR for DNA extracted from fresh, silica-dried, and herbarium plant material. "Since we study tropical plants, many of which are geographically restricted or rare," explains Samarakoon, "herbarium material is sometimes all that we have available for DNA extraction, and curators are gracious to allow even a small destructive sampling for a single extraction attempt. We want that one attempt, of course, to be successful." Samarakoon predicts that inhibitory plant compounds could be the underlying cause of many PCR failures in herbarium specimens and hopes TBT-PAR will have widespread benefits in herbarium specimen DNA amplification. 

TBT-PAR was first used in the PCR detection of a shrimp virus by co-author Shiao Wang and his colleagues. "The additive has also been helpful in a colleague's lab where they had trouble amplifying DNA from gopher tortoise ticks, so its utility extends beyond plants," comments Samarakoon. TBT-PAR has the potential for broad use in PCR techniques across DNA samples, species, and taxa.

The article will be published in the first issue of Applications in Plant Sciences (APPS), a new journal released by the Botanical Society of America. Theresa Culley, Editor-in-Chief of APPS, describes the new journal as a venue to "expedite the dissemination of innovative information encompassing all areas of the plant sciences, including but not limited to genetics, structure, development, evolution, systematics, and ecology."APPS publishes new methods in plant sciences -- an important niche to fill in an age of rapid technological advances.

Ready-to-use cocktail containing all components,except primer, for the amplification and detection of DNA in real-time quantitative PCR(qPCR).

The AccuPower® 2X Greenstar qPCR Master Mix is a ready-to-use cocktail containing all components,except primer, for the amplification and detection of DNA in real-time quantitative PCR(qPCR). It combines the automatic "Hotstart" technology of Top DNA polymerase and SYBR Green I fluorescent dye to deliver excellent sensitivity in the quantification of target sequences, with a linear dose response over a wide range of target concentration. Volumes are provided for 100 or 200 amplification reactions of 50ul each.

Features and Benefits

Applications

- Real-time quantification of DNA and cDNA targets
- Gene expression profiling
- Microbial & Viral pathogen detection

Applications

 

• Routine PCR applications
• TA cloning

Description

BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.

BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

BIOTAQ is supplied with 10x NH₄-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl₂ is provided to allow reaction conditions to be adjusted to suit the template.

The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.
 

Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

Concentration

5u/µl

Storage Conditions

BIOTAQ can be stored for 12 months at -20°C.
 

Product Citations

1. Amaral, I.P. & Johnston, I.A. J. Exp. Biol. 214, 2125-2139 (2011).
2. Coutinho, C.P., et al. Infect. Immun. 79, 2950-2960 (2011).
3. Lora, J., et al. PNAS 108, 5461-5465 (2011).
4. Palazón, A., et al. Can. Res. 71, 801-811 (2011).
5. del Hoyo, A. & Pedrola-Monfort, Plant Systemat. Evol. 273(3-4), 151-67 (2008).

Best price on the market: 118 Euro for 500 Units

Download datasheet

Common laboratory research practice utilizes PCR to validate transgenic mouse lines. Validation of these lines typically involve multiple primer sets with various annealing temperatures leading to a very tedious and time consuming process. To allow researchers the opportunity to evaluate multiple transgenes within one PCR reaction, Gentaur offers the MultiGene OptiMax. Traditional thermal cyclers utilize a Peltier microchip block that is enabled for either homogeneous or gradient temperature mode. Additionally, with a traditional thermal cycler, a user can only utilize one annealing temperature per experiment. The new Gentaur MultiGene OptiMax has six distinct Peltier microchip elements that allow users to select up to six different annealing temperatures. This allows for the possibility to evaluate multiple genes in one experiment.

• We now have a faster machine.
• Brand new better than gradient function.
• No more condensation issues
• Custom block optimisation
• Brand new PC Viewer

For more details:

 Operation Manual

Presentation

Optimax app note

Enduro Catalog ll

SPECIAL OFFER UNTIL THE END OF 2013

	ExiProgen™ is a breakthrough in synthetic biology allowing for the synthesis and purification of one to 16 proteins per run.  DNA, in the form of plasmid or linear PCR product, is added to the system and Gentaur’s coupled transcription/translation E. coli extract then synthesizes the protein.  The crude protein extract is further purified, using His-Tag engineered into the proteins.  The net result is up to 100 ug of >90% pure Protein in about 6 hours.  ExiProgen™ can also purify Nucleic Acids from several sources, making it one of the most versatile systems available.
Features and Benefits Built in protocols optimized for protein synthesis/purification and the extraction of a wide variety of nucleic acid samples ExiProgen™ has more contains over 900 protocols, each optimized for protein synthesis/purification and target nucleic acid type and source sample. This optimization enables the user to obtain reproducible results for every run, every day. The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols
Cooling Block ExiProgen™ has a built-in cooling block where the elution tube rack sits. Sample integrity is ensured by keeping the samples below 10°C. This allows for overnight runs and provides you with confidence in your results.
Magnetic block & Heating block To increase extraction efficiency, the ExiProgen™ has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. The heating block’s precision temperature control also ensures reproducible results for protein synthesis reactions.
Contamination Shield ExiProgen™ comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is a must when working with multiple samples.
Easy to use LCD Touch screen The 3.5" touchscreen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.
UV lamp ExiProgen™ has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.

Experimental Procedure

Principle of protein synthesis and purification
ExiProgen™ Protein Synthesis Kit useds a E. coli extract to effect coupled transcription/translation of input DNA, which can be plasmid, or PCR generated DNA. The protein itself is generated with a His-Tag, which is then purified using the Ni-NTA magnetic bead provided. The result is high yields of protein that is >90% pure.

Nucleic acid extraction principle
ExiProgen™ DNA/ RNA Kits work on the principle of cell lysis, followed by bind, wash elute from silica magnetic beads. High yields of ultrapure DNA or RNA are obtained with OD260 readings of > 1.8 for DNA and 2.0 for RNA.

And believe it or not it costs ONLY 15700 € !!!

AccuPower RocketScript Cycle RT PreMix is a ready-to-use lyophilized mastermix containing all components for first-strand cDNA synthesis from purified Poly(A) or total RNA template. 
The premix contains Gentaur's exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript, and oligo dT₂₀ for added convenience. Conditions are optimized for Gentaur's patented Cyclic Temperature Reverse Transcription (CTRT) in a premix form.

Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse 
transcription of RNA molecules with complex secondary structure. AccuPower RocketScript Cycle RT PreMix has thermostable activity across a wide temperature range, (42°C – 70°C), allowing efficient cDNA synthesis from virtually any RNA. 
The lyophilized PreMix contains all components necessary for a successful CTRT reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.

Schematic representation of the 5' UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.

Features and Benefits

Thermostable Activity: RocketScript is able to perform reverse transcription reactions throughout a wide range 
of temperatures from 42°C to 70°C. 

Enhanced Performance: RocketScript has enhanced performance to handle high and low input RNA concentrations 
as well as short and long RT target sizes. 

Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube. 

Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment. 

Flexible Reaction Conditions: Both CTRT and first-strand cDNA synthesis are both possible, with reaction temperatures 
ranging from 42°C to 70°C. 

Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided. 

Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer 

RNase, DNase and Proteinase-free: Ensures the integrity of your samples. 

Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure 

Stable for 2 years at -20°C: Long shelf life 

AccuPower RocketScript RT PreMix contains Gentaur’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript. Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure. 

RocketScript has thermostable activity (42°C~70°C), allowing efficient cDNA synthesis from virtually any RNA. The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.

Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail. 

Features and Benefits 

Thermostable Activity: RocketScript is able to perform reverse transcription reactions throughout a wide range 
of temperatures from 42°C to 70°C. 

Enhanced Performance: RocketScript has enhanced performance to handle both high and low input RNA 
concentrations as well as short and long RT target sizes. 

Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube. 

Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment. 

Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided. 

Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer 

RNase, DNase and Proteinase-free: Ensures the integrity of your samples. 

Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure 

Stable for 2 years at -20°C: Long shelf life 

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment Size: Up to 10 kb

Application

- First-strand synthesis of cDNA from RNA molecules (RT)
- RT-PCR
- Random priming reaction
- Library construction
- Probe labeling
- mRNA 5end Mapping by Primer Extension Analysis
- Real time PCR

Figure 1. Sensitivity comparison between AccuPower RocketScript RT PreMix and M-MLV RTase
Sensitivity results of AccuPower RocketScript RT PreMix using GAPDH compared with conventional Reverse transcriptases. 
Each 100 ng – 10 fg of total RNA used for RT and the same amount of RT products used for electrophoresis.
Lane 1: 10 fg Human total RNA from HeLa cell           Lane 2: 100 fg Human total RNA from HeLa cell
Lane 3: 1 pg Human total RNA from HeLa cell            Lane 4: 10 pg Human total RNA from HeLa cell
Lane 5: 100 pg Human total RNA from HeLa cell        Lane 6: 1 ng Human total RNA from HeLa cell
Lane 7: 10 ng Human total RNA from HeLa cell          Lane 8: 100 ng Human total RNA from HeLa cell

Figure 2. Comparison of amplification efficiency between AccuPower RocketScript RT PreMix and competitorsM-MLV RTase.
RocketScript is able to handle a wide range of sample concentrations and transcript lengths so your downstream applications are minimally effected by the reverse transcription step.
Lanes 1-3: 1,000 ng, 100 ng and 10 ng of total RNA from HeLa cells, respectively.

Figure 3. Sensitivity comparison between AccuPower RocketScript RT PreMix and competitor RTases using Real Time PCR
Reverse transcription conditions: conventional 1 hr incubation at 60°C, deactivation at 95°C for 5 min All cDNAs were amplified with AccuPower DualStar™ qPCR PreMix (K-6110) from Gentaur.

Concentration	RocketScript RT PreMix	Supplier Q	Supplier I
10,000	23.91	25.63	24.43
1,000	27.33	28.92	28.03
100	30.62	32.42	30.88
10	33.63	35.43	33.95
Efficiency	104%	103%	108%
Linearity	0.99999	0.9996	0.9995

 

The AccuPower RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix ofM-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal concentrations. Simply add template RNA, primers and water to start your reaction.

For cDNA amplification with Cyclic RT, please see our AccuPower CycleScript RT PreMix.

Features and Benefits