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GENTAUR Europe BVBA Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, Macedonia,
Montenegro, Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
Better than mTeSR
Pluri-EZ™ hESC/iPSC Culture Medium
Cat #: ASM-5014
Product Size: 100 ml
Background: The ability to culture human embryonic stem cells (hESCs) and Induced Plurpotent Stem Cells (iPSCs) under chemically defined conditions is a prerequisite not only for the production of hESCs/iPSCs under GMP conditions but, also the development of protocols that will be used for future preclinical/clinical studies (1). Attempts at the formulation of chemically defined tissue culture conditions have included: 1) The replacement of serum in the medium with a chemically defined substitute (2,3) and the replacement of a MEF feeder layer with a defined feeder free culture surface (4-6).
Figure 1: (a) iPSC passage 5 growth curves comparing Pluri-EZ™ to mTeSR™. No statistical difference was found in iPSC growth. Typical iPSC colony grown using Pluri-EZ™(b) and mTeSR™(c).
Product description: Pluri-EZ is chemical based media and highly stable.
Storage conditions: -20˚C; 1 year, 4˚C; 2 months. Minimize exposure to direct light.
Shipping: On dry ice
Notes:
1. Thaw the Pluri-EZ™ medium either overnight at 4˚C or for 20 minutes at RT.
2. Addition of bFGF and Activin A may help cell culture results.
Neuro-Sure™ Neural Crest Stem Cell Culture Media
Cat #: ASM-4021
Product Size: 100 ml
Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems 1,2. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells3,4. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
Product description and usage: Add 2 mL of NCSC medium supplement to 498 mL of
NCSC culture medium to produce 500 mL of NCSC media.
Storage conditions for NCSC medium: -80˚C; 1 year, 4˚C; two weeks. Minimize exposure
to direct light.
Storage condition for NCSC supplement: -80˚C; 1 year, 4˚C; two weeks.
Storage condition for NCSC media: 4˚C; two weeks. Minimize exposure to direct light.
Shipping: On dry ice.
Recommended procedure:
1. Thaw NCSC medium either overnight at 4˚C or for several hours at RT.
2. Thaw the NCSC medium supplement at RT.
3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4˚C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 370C water bath.
ESC-Sure™ Serum-/Feeder- Free hESC/iPSC Culture Medium (SFFM)
Cat #: ASM-5010
Product Size: 100 ml
Our Serum, feeder-free medium formulations are optimized for human ESC and iPSC culture. It contains growth factors and extracellular matrix proteins secreted by MEF cells.
SFFM is ready-to-use.
- Only need to add FGF2
- Culture dish coating: matrigel or similar matrix.
Blastocyst-Sure™ KSOM Embryo Culture Medium, with Phenol Red (with Amino Acid)
Cat #: ASM-5023
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)
Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.
Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.
Blastocyst-Sure™ KSOM Embryo Culture Medium, without Phenol Red (with Amino Acid)
Cat #: ASM-5024
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)
Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.
Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.