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    Tuesday, 17 December 2013 10:52

    Real Time PCR Molecular Diagnostics

    Catalogue RealTime PCR

    A Complete  Line for Several Applications

    TRANSPLANTATION LINE

    Name

     

    Code

     

    Description

     

    CMV DNA Quantitation (QT)

    CMVDNAQT.CE

    Quantitative Detection of CMV DNA

    HSV1 DNA Quantitation (QT)

    HSV1DNAQT.CE

    Quantitative Detection of HSV1 DNA

    HSV2 DNA Quantitation (QT)

    HSV2DNAQT.CE

    Quantitative Detection of HSV2 DNA

    HHV6 DNA Quantitation (QT)

    HHV6DNAQT.CE

    Quantitative Detection of HHV6 DNA

    HHV8 DNA Quantitation (QT)

    HHV8DNAQT.CE

    Quantitative Detection of HHV8 DNA

    EBV DNA Quantitation (QT)

    EBVDNAQT.CE

    Quantitative Detection of EBV DNA

    VZV DNA Quantitation (QT)

    VZVDNAQT.CE

    Quantitative Detection of VZV DNA

    BKV DNA Quantitation (QT)

    BKVDNAQT.CE

    Quantitative Detection of BKV DNA

    JCV DNA Quantitation (QT)

    JCVDNAQT.CE

    Quantitative Detection of JCV DNA

    ENTEROVIRUS RNA

    ENTERORNA.CE

    Detection of ENTEROVIRUS RNA

     

    EPATITIS DELTA

    Name

    Code

    Description

    HDV RNA Quantitation (QT)

    DRNA.CE

    Quantitative Detection of HDV RNA

     

    MISCELLANY

    Name

    Code

    Description

    DENGUE RNA

    DENGUERNA.CE

    Dengue RNA N e w

    CHAGAS DNA

    TCRUZIDNA.CE

    Chagas DNA N e w

    Toxoplasma gondii DNA

    TOXODNA.CE

    Detection of Toxoplasma gondii DNA

    Chlamydia trachomatis DNA

    CTDNA.CE

    Detection of Chlamydia trachomatis DNA

    Legionella pneumophila DNA

    LEPDNA.CE

    Detection of Legionella pneumophila DNA

     

    ACCESSORY  KIT

    Name

    Code

    Description

    RNA Retrotrascription Kit

    RNART.CE

    Kit for  the retrotrascription of RNA

    RNA HDV Retrotrascription Kit

    RNART.HDV.CE

    Kit for  the retrotrascription of RNA HDV only

     

    MAIN FEATURES

    • Kits contain all reagents necessary to carry out the test
    • Internal controls to verify successful DNA extraction and amplification
    • Quantitative tests: standard curve on 4 points
    • High  Sensitivity and Specificity
    • Small reaction volume: 5 ul
    • Sample types: blood, serum and plasma, Liquor, Biopsies
    • Storage temperature: +2/8°C (no  shipment in dry ice needed CE marked)
    • Shelf life: 12 months
    • Kits format: 25, 50, 100, 150 reactions: no waste

     

    PROTOCOLS

    Instruments protocols:

    • ABI 7500 Sequence Detection System® (Software SDS version 1.3.1, Applied Biosystems™)
    • Miniopticon® (Software CFX manager version 1.6, Biorad™)
    • MX3000P® (Software MxPro version 4.01,  Stratagene™)

    IN PROGRESS:
    - CFX 96 (BioRad™)
    - ROTOR-GENE Q (QIAGEN)
    - SmartCycler (CEPHEID)

     Order Button1 

    PDF-IconClick here for more information

    More: PCR

    Published in Promos

    toxoplasmainChronic infection with the parasite Toxoplasma gondii can make mice lose their innate, hard-wired fear of cats. This loss of their innate fear may persist after the parasite is no longer detectable in their brains, suggesting that initial infection may cause permanent changes in the mechanisms underlying their fear of predators. The results are published September 18 in the open access journal PLOS ONE by Wendy Ingram and colleagues from the University of California, Berkeley.
    The Toxoplasma parasite can be deadly, causing spontaneous abortion in pregnant women or killing immune-compromised patients, but it has even stranger effects in mice.
    Infected mice lose their fear of cats, which is good for both cats and the parasite, because the cat gets an easy meal and the parasite gets into the cat's intestinal track, the only place it can sexually reproduce and continue its cycle of infection.
    New research by graduate student Wendy Ingram at the University of California, Berkeley, reveals a scary twist to this scenario: the parasite's effect seem to be permanent. The fearless behavior in mice persists long after the mouse recovers from the flu-like symptoms of toxoplasmosis, and for months after the parasitic infection is cleared from the body, according to research published today (Sept. 18) in the journal PLOS ONE.
    "Even when the parasite is cleared and it's no longer in the brains of the animals, some kind of permanent long-term behavior change has occurred, even though we don't know what the actual mechanism is," Ingram said. She speculated that the parasite could damage the smell center of the brain so that the odor of cat urine can't be detected. The parasite could also directly alter neurons involved in memory and learning, or it could trigger a damaging host response, as in many human autoimmune diseases.
    Ingram became interested in the protozoan parasite, Toxoplasma gondii, after reading about its behavior-altering effects in mice and rats and possible implications for its common host, the domesticated cat, and even humans. One-third of people around the world have been infected with Toxoplasma and probably have dormant cysts in their brains. Kept in check by the body's immune system, these cysts sometimes revive in immune-compromised people, leading to death, and some preliminary studies suggest that chronic infection may be linked to schizophrenia or suicidal behavior.

    Pregnant women are already warned to steer clear of kitty litter, since the parasite is passed through cat feces and can cause blindness or death in the fetus. One main source of spread is undercooked pork, Ingram said.
    With the help of Michael Eisen and Ellen Robey, UC Berkeley professors of molecular and cell biology, Ingram set out three years ago to discover how Toxoplasma affects mice's hard-wired fear of cats. She tested mice by seeing whether they avoided bobcat urine, which is normal behavior, versus rabbit urine, to which mice don't react. While earlier studies showed that mice lose their fear of bobcat urine for a few weeks after infection, Ingram showed that the three most common strains of Toxoplasma gondii make mice less fearful of cats for at least four months.
    Using a genetically altered strain of Toxoplasma that is not able to form cysts and thus is unable to cause chronic infections in the brain, she demonstrated that the effect persisted for four months even after the mice completely cleared the microbe from their bodies. She is now looking at how the mouse immune system attacks the parasite to see whether the host's response to the infection is the culprit.
    "This would seem to refute – or at least make less likely – models in which the behavior effects are the result of direct physical action of parasites on specific parts of the brain," Eisen wrote in a blog post about the research.
    "The idea that this parasite knows more about our brains than we do, and has the ability to exert desired change in complicated rodent behavior, is absolutely fascinating," Ingram said. "Toxoplasma has done a phenomenal job of figuring out mammalian brains in order to enhance its transmission through a complicated life cycle."

    Published in News