Suppliers
Contact Us
 GENTAUR Europe BVBAVoortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR Spain
Tel 0911876558
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
This email address is being protected from spambots. You need JavaScript enabled to view it.
GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, 
Macedonia,
Montenegro, 
Croatia:
Tel 0035929830070
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.
GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.
Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
JETSTAR 2.0 Maxi
Description:
JETSTAR is a new and unique anion exchange resin
developed by Gentaur. This new resin is supplied in disposable
columns (Mini, Midi and Maxi columns) , which are used
under gravity flow conditions without further instrumentation.
The JETSTAR Plasmid Kits were designed by combining
JETSTAR Columns with a modified alkaline/SDS lysis proce-
dure for the preparation of plasmid DNA. Gentaur´s product
is the ideal tool to achieve ultrapure plasmid DNA within 45
Procedure :The procedure employs a modified alkaline/SDS method to prepare the cleared lysate. After neutral-ization, the lysate is applied onto a JETSTAR Column (Mini, Midi or Maxi) and the plasmid DNA is bound to the anion exchange resin . Washing the resin removes RNA and all other impurities. Finally the purified plasmid DNA is eluted from the column and concentrated by an alcohol precipitation.
Plasmid yields :Yields of up to 20 µg (Mini), 100 µg (Midi) and 500 µg (Maxi) of plasmid DNA can be expected using the JETSTAR columns. All types and sizes of plasmid DNA can be prepared, but yields depend very much on the plasmid copy numbers (low/medium/high), the type f plasmid, the bacterial strain and the volume of bacterial culture used. The recovery of plasmid DNA is on average between 85% and 90%.
Plasmid purity :The JETSTAR-purified plasmid DNA is of higher quality than 2 x CsCl purified plasmid DNA. Corre- spondingly, its recommended application range is extremely wide, including transfection, microinjection, vaccination, gene therapy, fluorescent and radioactive sequencing, amplification, in vitro transcription, cloning, etc.
Culture V olumes and DNA Yields
JETSTAR Kits are preferentially designed to extract and purify high copy plasmid DNA from E.coli cultures. Low copy plasmids can be prepared as well, but larger culture volumes have to be used.
Gentaur recommends LB medium to grow E.coli cells to prepare plasmid DNA with JETSTAR columns. The JETSTAR system is however compatible also with other growth media, especially those designed for enhanced plasmid yields . The cell density should be approximately 1 x 10 cells per ml medium (1-1.5 A 600 units/ml).
High Copy Plasmids (3 - 5 µg DNA/ml LB medium)
| A. | Culture Volume | DNA Yield |
| Mini | 1 -5 ml | 3 - 20 µg |
| Midi | 15 - 25 ml | 45 - 100 µg |
| Maxi | 100 ml | 300 - 500 µg |
Low Copy Plasmids (0.2 - 1 µg DNA/ml LB medium)
| A. | Culture Volume | DNA Yield |
| Mini | 10 - 15 ml | 2 - 45 µg |
| Midi | 25 - 100 ml | 5 - 100 µg |
| Maxi | 250 - 500 ml | 50 - 500 µg |
Product pricelist:
| Product | Contents | Cat.# | Price: |
| JETSTAR 2.0 Plasmid Midiprep Kit / 25 | 25 Midi Columns Solutions, Reagents |
2210025 | 126 € |
| JETSTAR 2.0 Plasmid Midiprep Kit / 50 | 200 Midi Columns Solutions, Reagents |
210050 | 202 € |
| JETSTAR 2.0 Plasmid Midiprep Kit / 250 | 250 Midi Columns Solutions, Reagents |
210250 | 721 € |
| JETSTAR 2.0 Plasmid Midiprep LFU Kit / 25 | 50 Midi Columns Solutions, Reagents |
211025 | 186 € |
| JETSTAR 2.0 Plasmid Midiprep LFU Kit / 100 | 10 Midi Columns Solutions, Reagents |
211100 | 547 € |
| JETSTAR 2.0 Plasmid Maxiprep Kit / 10 | 10 Maxi Columns Solutions, Reagents |
220010 | 126 € |
| JETSTAR 2.0 Plasmid Maxiprep Kit / 20 | 20 Maxi Columns Solutions, Reagents |
220020 | 202 € |
| JETSTAR 2.0 Plasmid Maxiprep Kit / 100 | 100 Maxi Columns Solutions, Reagents |
220100 | 769 € |
| JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 20 | 20 Maxi Columns Solutions, Reagents |
221020 | 289 € |
