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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
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    GENTAUR France

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    Gentaur London

     GENTAUR Ltd. 
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    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
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    Kuiper 1 
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
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    Genprice USA

    usa-flagGenprice Inc, Logistics
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    San Jose, CA 95123
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    San Jose, CA. 96123

     

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    Tel 00302111768494 
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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
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    Monday, 08 April 2013 10:14

    Avian influenza H7N9 Promo

    uscn smallDear Clients, 

    We are happy to announce that another promo is available as of today: 

    1. Intended Use 

    Avian influenza virus H7N9 real time RT-PCR kit is used for the detection of gene H7 and gene N9 of avian influenza A subtype H7N9 in human nasal and pharyngeal secretions and bird fece by using real time PCR systems.

    2. Principle of Real-Time PCR

    The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities during Real Time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.

    3. Product Description

    Highly pathogenic avian influenza (HPAI) caused by certain subtypes of influenza A virus in animal populations, particularly chickens, poses a continuing global human public health risk. Direct human infection by an avian influenza A (H5N1) virus was first recognized during the 1997 outbreak in Hong Kong. The avian influenza virus H7N9 is one subgroup among the larger group of H7 viruses. Some cases of human infection with H7N9 virus in China are confirmed till early April of 2013.

    Avian influenza virus H7N9 real time RT-PCR kit contains a specific ready-to-use system for the detection of avian influenza virus H7N9 by Reverse Transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The master contains Super Mix for the specific amplification of the avian influenza virus H7N9 RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the avian influenza virus H9 RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by polymerase chain reaction. Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The detection of amplified avian influenza virus H7N9 DNA fragment is performed in fluorimeter channel FAM and HEX/VIC/JOE with the fluorescent quencher BHQ1. In addition, the kit contains a system to identify possible PCR inhibition by measuring the FAM fluorescence of the internal control (IC).

     4. Kit Contents

    Ref.

    Type of reagent

    Presentation 25rxns

    1

    2

    3

    4

    H7N9 Super Mix

    RT-PCR Enzyme Mix

    Molecular Grade Water

    H7N9 Positive Control  

    1 vial, 480ml

    1 vial, 28ml

    1 vial, 400μl

    1 vial, 30μl

    Analysis sensitivity: 1×103copies/ml;

    Note: Analysis sensitivity depends on the sample volume, elution volume, nucleic acid extraction methods and other factors .If you use the RNA extraction kits recommended, the analysis sensitivity is the same as it declares. However, when the sample volume is dozens or even hundreds of times greater than elution volume by some concentrating method, it can be much.

     

    Our Price: EUR 562

    Order Button1

     

    For additional information, here is a full view of Gentaur's AIV-related products: 

    http://antibody-antibodies.com/search_full.php?search=AIV

    Published in Promos
    Friday, 05 April 2013 16:15

    Seasonal Discount

    bottlesweb

    Dear Clients,

    From the 1st May until 30th June we will be offering 20% off the list price of our flashBAC kits.

     

    This includes:

    flashBAC 3,5,24 reaction kits.

    flashBAC GOLD 3,5,24 reaction kits.

    flashBAC ULTRA 3,5,24 reaction kits

    flashBAC PRIME 3,5,24 reaction kits.

     

    Hurry and call us today to secure your product!

    Published in Promos

    RNA-targatt-antibody-antiA single drug may be an effective therapy for a number of different viral diseases such as Ebola and rabies, a new study published in Cell Chemistry and Biology.

    John Connor - a virologist at the University of Boston, USA, and co-author of the article, explains that his research team study the vesicular stomatitis virus, a close relative of the Ebola virus, but not as deadly. It turns out that several viruses, including rabies, mumps, vesicular stomatitis virus and NICA (deadly pathogen spread by bats) use the same method to reproduce in human cells. This led scientists to start looking for a substance that can stop the replication process of these viruses.

    The result - the first broad-spectrum antiviral compound that stops the playback of a variety of viruses through disruption of the synthesis of viral ribonucleic acid. Although up to a medicinal product used in humans will probably take at least several years of laboratory research and as many clinical studies, the discovery is a major breakthrough in anti-infective and, in particular - antivirus, therapy with the potential to change the treatment of many of the most serious viral diseases.

     

    Published in News

    12846 bigFDA issued a permit for use of the new product Tenex Health - TX1 - a system to remove damaged or necrotic tissue. This application will allow a wide range of surgical procedures and interventions - abdominal surgery, orthopedic surgery, laparoscopy, Plastic and Reconstructive Surgery.

    The company currently offers products for the treatment of damaged tissue in the tendons in chronic tendon pain. INSTRUMENTS is the size of a pen and allows surgeons to supply energy in the form of ultrasonic waves through a needle that cuts and removes only the dead tissue. The whole procedure was carried out for 20 minutes under local anesthetic.

    Each year in the U.S. alone, the number of operations in tendon pain is over 10 million. Extending the testimony of multiple device will increase its applicability and convenience for physicians and patients, and the quality of the manipulation.

    According to Jill Foosball - Executive Director of Tenex Health, the device allows surgery at an early stage of the disease, allowing for faster, easier and more effective treatment and faster return patients to their daily activities. He said TH1 offers unique advantages - minimum invasiveness, which means speed, efficiency and safety of the procedures with minimal recovery time.

    The company designs and other products for minimally invasive surgery for the treatment of soft tissue injuries when bursitis, carpal tunnel surgery syndrome and post surgical adhesions.

    Published in News

    gene-therapy-gentaur-antibodiesWITHIN just eight days of starting a novel gene therapy, David Aponte's "incurable" leukaemia had vanished. For four other patients, the same happened within eight weeks, although one later died from a blood clot unrelated to the treatment, and another after relapsing. The cured trio, who were all previously diagnosed with usually fatal relapses of acute lymphoblastic leukaemia, have now been in remission for between 5 months and 2 years. Michel Sadelain of the Memorial Sloan-Kettering Cancer Center in New York, co-leader of the group that designed the trial, says that a second trial of 50 patients is being readied, and the team is looking into using the technique to treat other cancers.

    The key to the new therapy is identifying a molecule unique to the surface of cancer cells, then genetically engineering a patient's immune cells to attack it. In acute lymphoblastic leukaemia, immune cells called B-cells become malignant. The team were able to target a surface molecule known as CD19 that is only present on B-cells. Doctors extracted other immune cells called T-cells from the patients. These were treated with a harmless virus, which installed a new gene redirecting them to attack all cells bearing CD19. When the engineered T-cells were reinfused into the patients, they rapidly killed all B-cells, cancerous or otherwise.

    "The stunning finding was that in all five patients, tumours were undetectable after the treatment," says Sadelain. He reckons that the body should replenish the immune system with regular T-cells and healthy B-cells after a couple of months. However, the patients received donated bone marrow to ensure they could regrow a healthy immune system.

    The treatment is not the first to re-engineer T-cells to attack a form of leukaemia. Last year, an international company called Adaptimmune used the approach to treat 13 people with multiple myeloma – it left 10 in remission. "Although it's early days for these trials, the approach of modifying a patient's T-cells to attack their cancer is looking increasingly like one that will, in time, have a place alongside more traditional treatments," says Paul Moss of Cancer Research UK. Sadelain's team is now investigating the scope for attacking other cancers. Where no single surface molecule is unique to a cancer, he is seeking to target pairs of molecules that only occur together on cancer cells. In January, he demonstrated this approach by wiping out human prostate tumours implanted in mice, using T-cells engineered to target two surface molecules.

     

    Published in News

    french-bees-honey-gentaurThe hands of French apiarist Andre Frieh hold jars of coloured honey at his home in Ribeauville near Colmar Eastern France, October 5, 2012. (Reuters/Vincent Kessler)

     

    Beekeepers in north eastern France have been scratching their heads after the hives began to produce a weird colored substance instead of regular honey. They think candy M&Ms are to blame.

    The bees around the town of Ribeauville in the Alsace region have been carrying an unidentified colored substance back to their hives since August. The keepers have done a bit of sleuthing and think the Agrivolar biogas plant around 4 kilometers away is to blame.

    The enterprise has been processing waste from a Mars factory producing the colored M&M’s. The waste products have been stored in open containers and the bees could easily access the contents.

    “We discovered the problem at the same time they did. We quickly put in place a procedure to stop it,” Reuters quotes Agrivalor manager Philippe Meinrad as saying. The plant said they would now store waste indoors and in tightly closed containers.

    The beekeepers have already suffered high bee mortality due to the coldness of last winter. They are now wondering what to do with the colored honey and whether their bees will survive after dealing with the chemicals, Alain Frieh, president of the apiculturists’ union said. Also they will have to face a decline in sales, as they won’t be able to make much money out of the blue and green honey.

    “For me, it’s not honey. It’s not sellable,” Frieh says adding that the substance still tastes like honey.

    France is among the major producers of honey in the EU. There are around 2,400 beekeepers in the Alsace region producing about 1,000 tons of honey per year, according to the region’s chamber of agriculture.

    french-bees-honey-gentaur-2

     

    Published in News

    While providing a thorough overview of the observed objects, optical microscopes are limited by so-called. diffraction barrier, why microscope can not distinguish two separate objects if they are at a distance of less than about 200 nm. This microscope is not simple, says Gizmodo.

    Combining powerful optics and advanced algorithms to recreate the image, DeltaVision OMX Blaze General Electric Company allows us to peer into the microscopic world and remain amazed by it.

    Established in 2011, DeltaVision OMX Blaze worth 1.2 million dollars. "Some of us jokingly started calling him OMG, after seeing images that can produce it," says Jane Stout of the Medical Faculty of the University of Indiana in Bloomington.

    In footage shown here winning Elestric General Healthcare Life Sciences 2012 Imaging Competition.

    hek293 cells expressing fluorescent gpcr  green  and   -arrestin  red  fusion proteins with dna staining  blue . therapeutic focus drug discovery gentaur

    gentaur-antibodies-cell-culture-microscope-4

    gentaur-antibodies-cell-culture-microscope-3

    gentaur-antibodies-cell-culture-microscope-2

    Published in News

    horror frog"Amphibian horror" isn't a movie genre, but on this evidence perhaps it should be. Harvard biologists have described a bizarre, hairy frog with cat-like extendable claws.Trichobatrachus robustus actively breaks its own bones to produce claws that puncture their way out of the frog's toe pads, probably when it is threatened. David Blackburn and colleagues at Harvard University's Museum of Comparative Zoology, think the gruesome behaviour is a defence mechanism. The researchers say there are salamanders that force their ribs through their skin to produce protective barbs on demand, but nothing quite like this mechanism has been seen before. The feature is also found in nine of the 11 frogs belonging to the Astylosternusgenus, most of which live in Cameroon.

    Instant weapon

    "Some other frogs have bony spines that project from their wrist, but in those species it appears that the bones grow through the skin rather than pierce it when needed for defence," says Blackburn. At rest, the claws of T. robustus, found on the hind feet only, are nestled inside a mass of connective tissue. A chunk of collagen forms a bond between the claw's sharp point and a small piece of bone at the tip of the frog's toe. The other end of the claw is connected to a muscle. Blackburn and his colleagues believe that when the animal is attacked, it contracts this muscle, which pulls the claw downwards. The sharp point then breaks away from the bony tip and cuts through the toe pad, emerging on the underside.

    Hirsute horror

    The end result may look like a cat's claw, but the breaking and cutting mechanism is very different and unique among vertebrates. Also unique is the fact that the claw is just bone and does not have an outer coating of keratin like other claws do. Because Blackburn has only studied dead specimens, he says he does not know what happens when the claw retracts - or even how it retracts. It does not appear to have a muscle to pull it back inside so the team think it may passively slide back into the toe pad when its muscle relaxes. "Being amphibians, it would not be surprising if some parts of the wound heal and the tissue is regenerated," says Blackburn. Males of the species, which grows to about 11 centimetres, also produce long hair-like strands of skin and arteries when they breed (see image). It is thought that the "hairs" allow them to take in more oxygen through their skin while they take care of their brood.

    Spiky snack

    In Cameroon, they are roasted and eaten. Hunters use long spears and machetes to kill the frogs, apparently to avoid being hurt by their claws. "This is an incredible story," says Ian Stephen, curator of herpetology at the Zoological Society of London, UK. "Some frogs grow spines on their thumbs during breeding season, but this is entirely different." "For me, it highlights the need for a lot more research on amphibians especially in light of the threat of mass extinctions," he adds. The existence of frogs with erectile claws like cats was first described by Belgian zoologist George Boulenger in 1900 in frogs found in the French Congo, now the Republic of Congo.

     

    Published in News
    Thursday, 28 March 2013 16:39

    XerumFree™ XF205 Medium Supplement

    Adapting Cells To a Serum-Free Environment

    Fully defined, animal-component free and GMP produced cell culture supplement.

    Performing cell culture without serum can be challenging. However, the rewards do largely recompense the efforts, and re-discove- ring the basics of cell culture develops quickly into a passion. The intention of this paper is to guide the user to a smooth transition to serum-free conditions and to avoid all inadequate or inappropriate efforts.

    Ideally, the transition to serum-free conditions should be carried out over several passages to gradually select cells that can grow under serum-free conditions. However, direct adaptation to serum-free environments may also work out successfully, provided that all crucial aspects are addressed properly.

    Regardless of the method used, key concerns include the growth state of the cellular inoculum, cell seeding density, sub-cultivation techniques, and biophysical attributes of the cell culture system.

    Our XerumFree™ serum replacement has been designed so as to be used in the same way as conventional cell culture sera, as a medium supplement. The concentration however is 5x higher, so typically you will use 2% to a basal medium. You will go through the same steps as usual.

    Be the first to order this brand new product!

    Order Button1

    PDF-IconDownload datasheet with instructions for use

    Published in Promos
    Thursday, 28 March 2013 11:07

    Better than mTeSR

    Pluri-EZ™ hESC/iPSC Culture Medium

    Cat #: ASM-5014
    Product Size: 100 ml
    Background: The ability to culture human embryonic stem cells (hESCs) and Induced Plurpotent Stem Cells (iPSCs) under chemically defined conditions is a prerequisite not only for the production of hESCs/iPSCs under GMP conditions but, also the development of protocols that will be used for future preclinical/clinical studies (1). Attempts at the formulation of chemically defined tissue culture conditions have included: 1) The replacement of serum in the medium with a chemically defined substitute (2,3) and the replacement of a MEF feeder layer with a defined feeder free culture surface (4-6).

    targatt

    Figure 1: (a) iPSC passage 5 growth curves comparing Pluri-EZ™ to mTeSR™. No statistical difference was found in iPSC growth. Typical iPSC colony grown using Pluri-EZ™(b) and mTeSR™(c).
    Product description: Pluri-EZ is chemical based media and highly stable.
    Storage conditions: -20˚C; 1 year, 4˚C; 2 months. Minimize exposure to direct light.
    Shipping: On dry ice

    Notes:
    1. Thaw the Pluri-EZ™ medium either overnight at 4˚C or for 20 minutes at RT.
    2. Addition of bFGF and Activin A may help cell culture results.

    Neuro-Sure™ Neural Crest Stem Cell Culture Media

    Cat #: ASM-4021
    Product Size: 100 ml
    Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems 1,2. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells3,4. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
    Product description and usage: Add 2 mL of NCSC medium supplement to 498 mL of
    NCSC culture medium to produce 500 mL of NCSC media.

    Storage conditions for NCSC medium: -80˚C; 1 year, 4˚C; two weeks. Minimize exposure
    to direct light.

    Storage condition for NCSC supplement: -80˚C; 1 year, 4˚C; two weeks.

    Storage condition for NCSC media: 4˚C; two weeks. Minimize exposure to direct light.

    Shipping: On dry ice.

    Recommended procedure:
    1. Thaw NCSC medium either overnight at 4˚C or for several hours at RT.
    2. Thaw the NCSC medium supplement at RT.
    3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4˚C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
    4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 370C water bath.

     

    ESC-Sure™ Serum-/Feeder- Free hESC/iPSC Culture Medium (SFFM)

    Cat #: ASM-5010
    Product Size: 100 ml
    Our Serum, feeder-free medium formulations are optimized for human ESC and iPSC culture. It contains growth factors and extracellular matrix proteins secreted by MEF cells.

    SFFM is ready-to-use.
    - Only need to add FGF2
    - Culture dish coating: matrigel or similar matrix.

     

    Blastocyst-Sure™ KSOM Embryo Culture Medium, with Phenol Red (with Amino Acid)

    Cat #: ASM-5023
    Product Size: 1 Pack (8 ml X 3 vials)
    Size: 1 Pack (8 mL X 3 vials)

     

    Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.

    targatt

    Formulation: Frozen liquid
    Recommended protocol:
    1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
    2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
    3. Change to fresh medium every 24h during embryo culture.

     

     

    Blastocyst-Sure™ KSOM Embryo Culture Medium, without Phenol Red (with Amino Acid)

    Cat #: ASM-5024
    Product Size: 1 Pack (8 ml X 3 vials)
    Size: 1 Pack (8 mL X 3 vials)

     

    Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.

    targatt

    Formulation: Frozen liquid
    Recommended protocol:
    1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
    2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
    3. Change to fresh medium every 24h during embryo culture.

    Published in Promos