Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

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    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Monday, 11 March 2013 15:47

    Stem Cell Culture Services

    We offer standard and customized cell culture services including mycoplasma testing and FBS lot evaluation.

    Please contact us to discuss your needs with our technical service specialists.

    Published in Targatt services
    Monday, 11 March 2013 15:44

    Stem Cell Differentiation

    Targatt offers various services for the differentiation of ESC/iPSC lines into more specialized cells including multipotent stem cells and fully differentiated somatic cells.

    Advantage of using multipotent stem cells

    • Thaw ESC/iPSC-derived multipotent stem cells (e.g. Neural Stem Cells) when you need them
    • Differentiate multipotent stem cells to specialized somatic cells (e.g. neurons and glial cells)
    • Expand the multipotent stem cells for a ready supply for your planned experiments = save money and time!

    Standard services:

    • Neural Stem Cells
    • Neural Crest Stem Cells
    • Cardiomyocytes
    • Skeletal Muscle

     

    Bespoke services:

    We also offer customized differentiation services. Please contact us with details about what kind of cells you are interested in.

     

    Human ESC / iPSC Neuronal Differentiation Service

    • Send us your pluripotent cell line (or use our iPSC generation service)
    • We will differentiate your ESC/iPSC line into neural stem cells (NSCs) or neural crest stem cells (NCSC) by using our proprietary neural induction protocol.
    • NSCs and NCSCs are proliferating cells that can be frozen and cultured over a prolonged period of time.
    • NSCs can be further differentiated into multiple neural cell types.

     

    stemcell-targatt-antibodies-gentaur-4

    Published in Targatt services
    Monday, 11 March 2013 15:39

    Teratoma Formation Analysis Services

    Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.

    Features:

    • - Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
    • - Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
    • - Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

     

    Service Includes:

    • - Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
    • - Tissue blocks and H&E stained tissue section slides

     

    Notes:

    • - Frozen sample culture 
    • - Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) 
    • - Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request.
    • - Frozen tissue blocks are available.

     

    Technical Details:

    • Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
    • Injection site:Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
    • Service includes:Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.

    teratoma-gentaur-targatt-1

    teratoma-gentaur-targatt-2

    teratoma-gentaur-targatt-3

    Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.

    Features:

    • Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
    • Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
    • Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

    Service Includes:

    • Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
    • Tissue blocks and H&E stained tissue section slides

    Notes:

    • Frozen sample culture Fee $500 per line
    • Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) $200 per line
    • Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request with additional $100 per line.
    • Frozen tissue blocks are available with additional $100.
    Published in Targatt services
    Monday, 11 March 2013 15:36

    Stem Cell Characterization

    Human and Mouse ESC/iPSC Characterization in vivo & in vitro

    • Teratoma Formation Analysis Service
    • EB Formation and Analysis Service
    • Pluripotency Marker Characterization (Human / Mouse)
    • Karyotyping (Human / Mouse)
    • Germline Transmission of mouse ESCs
    • Sex determination

    We make the line for you, and we characterize it, too!

    We offer a wide range of services to help you identify and characterize stem cell lines. 

    stemcell-gentaur-targatt-antibodies

    Published in Targatt services
    Monday, 11 March 2013 15:33

    Stem Cell Generation

    We have successfully derived many induced pluripotent stem cell (iPSC) lines from patient specific fibroblasts.

    We have three methods for iPSC generation: mRNA, episomal (both genomic footprint-free), or retroviral.

    Genomic footprint-free Induced Pluripotent Stem Cell (IPSC) Generation Service:

    • - No genomic footprint - the non-integrating system is safe in drug discovery and cell therapy applications.
    • - Two methods available: Episomal DNA vectors containing the reprogramming factors or mRNA.
    • - High reprogramming efficiency (0.2%-0.5%).
    • - The reprogramming vectors/mRNA are removed naturally during the cell cycle.
    • - Safe to handle - no viral particles.

     

    • stemcell-targatt-knockin-knockout-mouse-1
    Published in Targatt services
    Monday, 11 March 2013 15:30

    Stem Cell Derivation

    Targatt offers a mouse embryonic stem cell (ESC) derivation service from any strain of your interest. We can also derive ESC lines from transgenic mice.

    Published in Targatt services
    Monday, 11 March 2013 15:26

    Cell Line Gene Modification

    Cell line gene modification is a versatile genetic tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications.

    Gene targeting is primarily performed in embryonic stem (ES) cells for the purpose of generating knock-out mice. However, targeted gene modification in cultured cell lines can be a considerably easier and more rapid approach of creating valuable research models. Customized gene-targeted mammalian cell lines are a cheap and convenient model system that can be used for many applications in research and drug discovery, including gene function studies and high-throughput screening.

    With strong expertise in genetic modification, gene targeting and cell culture, Targatt can help you to make your customized gene modified cell line. We specialized in both adherent and suspension cell culture. We have many traditional mammalian cell lines in stock and we can also adapt our established targeting techniques to less commonly used cell lines. With a great variety of tools for cell line gene modification in place we have successfully generated gene-targeted clonal lines even from hard-to-transfect cells.

    Available Gene Modification Strategies (selected):

    • Knock-in/knock-out
    • Gene replacement
    • Gene editing
    • Gene therapy
    • Mutagenesis

    Each project is individually devised and realized. Our service includes an initial project assessment based on your model requirements. A dedicated PhD level project manager will discuss available gene targeting methods, vector design strategies and special culture requirements with you. After targeting we will clonally expand and cryopreserve several stable transgenic cell lines. Upon request we can expand the clones further on a large scale for high-throughput assays.

    Applications (selected)

    • - Recombinant protein production in CHO cells
    • - Disease model in human cell lines for drug discovery
    • - Gene therapy in diseased cell line
    • - Generation of a specific cell line with TARGATT docking site as a master cell line for site-specific gene knock-in reporter cell line for gene function studies

     

    Targatt also offers additional services wtihin our cell line models line including TARGATT Gene Insertion.

    Published in Targatt services

    Based on our proprietary TARGATT Technology for site-specific knock-in mouse generation, we developed a TARGATT system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted efficiently into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.

     TARGATT Features:

    • Site-specific insertion of any gene 
    • High integration efficiency
    • Single-copy integration
    • Stable expression
    • Fast: get your transgenic cell line in 3 months!

     

    targatt talen knockout genemodification

    targatt-antibodies-gentaur-knockin-knockout-mouse-1

    Targatt proprietary TARGATT Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Our TARGATT Technology improves several aspects in the generation of transgenic cell lines and animals:

    1. High integration efficiency mediated by  PhiC31 integrase reduces time and cost
    2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene
    3. Integration at intergenic region ensures that no internal genes are interrupted
    4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability
    5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

    TARGATT Technology can be utilized for a variety of applications including reporter gene expression, gene knockdown and disease models.

    targatt-antibodies-gentaur-knockin-knockout-mouse-3

    Published in Targatt services
    Monday, 11 March 2013 15:15

    Knock-in and Knock-out Rat

    Targatt provides a complete range of gene targeting services in rats to generate transgenic knock-in and knock-out rat models.

    Rats are more versatile research model animals than mice since they are physiologically more similar to humans. The recent isolation of rat embryonic stem (ES) cells opened the doors to the generation of transgenic rats and we are proud to be one of the first providers of an all-inclusive transgenic rat generation service. Our competence in gene-targeting vector design coupled with our expertise in culturing rat ES cells allows us to offer a high-quality customized service for the generation of transgenic knock-in and knock-out rats.

    Using our proprietary germline competent rat ES cell lines we can create your customized transgenic rat model, including

    • - Constitutive knockin/out
    • - Conditional knockin/out
    • - Inducible knockin/out
    • - Point mutations
    • - Random integration
    • - Insertion of reporter genes
    • - Humanized rat models
    • - Disease rat models
    • knock-in-knock-out-mouse-targatt-1

     

    We take great pride in offering you the highest level of service and we are committed to ensuring the success of your transgenic rat project. Our service includes an initial project assessment based on your model requirements. A dedicated PhD level project manager will discuss available gene targeting vector design strategies and will update you continuously throughout the project.

    We provide the most flexible customized service options whether you’d like us to do all the work or are on a tight budget and prefer doing some steps yourself.

     Full Transgenic Rat Generation Service Includes:

    • - Gene targeting vector design, construction and sequencing
    • - Rat ES cell targeting, screening and expansion of positive rat ES cell clones
    • - Karyotyping of targeted rat ES cells
    • - Chimera production via blastcyst injection
    • - Chimera breeding for germline transmission
    • - Genotyping of offspring
    • - Transfer of heterozygous targeted rats to the customer
    • - Optional: breeding to generate homozygous rats

     

    All our animals are housed in a facility that is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International, has an Assurance on file with the Office of Laboratory Animal Welfare (OLAW), and is a registered research facility with the U.S. Department of Agriculture.

    knock-in-knock-out-mouse-targatt-2

    We also offer Knock-in/Knock-out Mouse and Transgenic Mouse (Random Insertion) services. For more information please contact us.

     

    Published in Targatt services
    Monday, 11 March 2013 15:13

    Random Transgenic Mouse

    We provide a service to generate transgenic mice by traditional pronuclear microinjection. Using this method, transgenes are inserted randomly into the genome.

    Expression of the transgene and the subsequent transgenic mouse phenotype may vary from line to line due to the nature of random gene insertion. We recommend to generate multiple independent trangenic mouse lines in order to interpret the experimental results.

    Available mouse strains: FVB, C57BL/6

    We also offer Knock-in and Knock-out Mice and Knock-in and Knock-out Rat services. For more information please contact us.

    Generation of transgenic mice by random insertion

    Step 1: Cloning of cDNA, promoter of interest, and other elements

    Step 2: Construction of the transgene vector

    Step 3: Pronuclear microinjection of vector DNA into mouse embryos

    Step 4: Transfer of injected embryo into recipient mothers

    Step 5: Genotyping of offspring to identify potential transgenic founders

    Step 6: Breeding to F1 or F2 progenies (optional)

    Published in Targatt services