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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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 GENTAUR BULGARIA
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    Background staining or non-specific staining is an often-encountered problem in immunohistochemistry, in immunofluorescence and in situ stains. Background staining is caused by a number of factors such as cross reactivity of antibodies with the shared epitopes in the tissue, by the presence of natural and/or contaminating antibodies present in the primary antibody and/or the secondary antibody, by ionic interactions, by the presence of carbohydrates and by endogenous biotin present in the tissue. Eradicating background is most important for obtaining background-free specific staining for the ease of qualitative and quantitative evaluation.

    PRODUCT DESCRIPTION
    Background Buster is a peptide Blocker that eradicates all general background staining. Background Buster removes all background staining caused by primary antibodies, by the staining reagents, by the chromogens, by the fixatives and by endogeneouse biotin present in tissues such as liver and spleen and kidney. Background Buster is used in place of normal sera and other blocking solution for removing background staining in both human and animal tissues.

    Background Buster is applicable IHC staining, to immunofluorescence staining and to in situ probe staining in both human and animal tissues. It is also applicable to flow cytometric assays.

    Background Buster is a must for animal tissue staining, it is especially essential when staining identical species tissue and antibodies such as mouse antibodies on mouse tissues (Mouse-on-Mouse) and Rabbit-on- Rabbit. A 30-minute incubation with Background Buster is recommended prior to the application of the primary antibody for staining of identical species primary antibodies and tissues. The use of Background Buster is highly recommended for staining of indirect species (non-identical species tissue/ antibody) such as Rat –On-Mouse, Mouse-on- Rat, Mouse-on-Rabbit, etc. A 20-minute incubation with Innovex Background Buster is recommended prior to the application of the primary antibody for staining of Indirect (non-identical species primary antibodies and tissues).

    In immunoperoxidase-IHC staining, another type of background and non-specific staining is caused by red blood cell staining; this is due to endogenous peroxidase enzyme present in red blood cells. This type of background requires a pre-treatment step with 3% freshly made hydrogen peroxide (H2O2) in water, this blocking step should precede the blocking step with Innovex Background Buster.

    NB306 Background Buster 125 ml qty 419 EUR
    NB306-50 Background Buster 50 ml qty 325 EUR

    For more information Download PDF file

    Immunolocalization of electrogenic sodium-bicarbonate cotransporters pNBCI and kNBCI in the rat eye

    IMMNLINOLOCALIZATION  OF SODIUM.BICARBONATE  COTRANSPORTERS

    More: Background Buster

    Published in Promos


    A MUST FOR:

    • - Mouse-On-Mouse
    • - Human and Animal IHC
    • - Immunofluorescence
    • - in-situ Hybridization


    background busterBackground Buster is highly effective for quenching background fluorescence and is easy to use: 

    For Fluorescence:

    • Apply Background Buster for 15 minutes prior to application of the first antibody (unlabeled or fluorescence-labeled antibody).
    • Rinse with PBS for 1 minute.
    • Apply unlabeled antibody for indirect method or fluorescence labeled antibody for direct method and continue with usual protocol.

     

    Background Buster can be used for IHC:

     background buster ihc

     

    Background or non-specific staining is often observed in a variety of immunoassays, in immunohistochemistry and other immunoassay types such as immunofluorescence, ELISA and flow cytometric assays, background staining can be prevented by the use of INNOVEX Recombinant Protein Technology,Background Buster.


    Background Buster is also applicable to eliminating non-specific binding in immunofluorescence, ELISA and flow cytometric assays.


    ADVANCED & UNIQUE FEATURES:

    • Allows staining of identical species antibodies and tissues (e.g., mouse antibody on mouse tissue, rat-on-rat, rabbit-on-rabbit, etc.).
    • Short 10 minute incubation step prior to applying primary antibody or in-situ probe at room temperature
    • Delivers complete eradication of general background staining
    • Replaces the use of normal serum, powdered milk, casein, and other blocking agents and renders complete success
    • Excellent for both frozen and paraffin sections
    • Excellent for in situ application
    • A must for animal tissue staining

    Download datasheet PDF file

    Published in Promos