Pluri-EZ™ hESC/iPSC Culture Medium

Cat #: ASM-5014
Product Size: 100 ml
Background: The ability to culture human embryonic stem cells (hESCs) and Induced Plurpotent Stem Cells (iPSCs) under chemically defined conditions is a prerequisite not only for the production of hESCs/iPSCs under GMP conditions but, also the development of protocols that will be used for future preclinical/clinical studies (1). Attempts at the formulation of chemically defined tissue culture conditions have included: 1) The replacement of serum in the medium with a chemically defined substitute (2,3) and the replacement of a MEF feeder layer with a defined feeder free culture surface (4-6).

Figure 1: (a) iPSC passage 5 growth curves comparing Pluri-EZ™ to mTeSR™. No statistical difference was found in iPSC growth. Typical iPSC colony grown using Pluri-EZ™(b) and mTeSR™(c).
Product description: Pluri-EZ is chemical based media and highly stable.
Storage conditions: -20˚C; 1 year, 4˚C; 2 months. Minimize exposure to direct light.
Shipping: On dry ice

Notes:
1. Thaw the Pluri-EZ™ medium either overnight at 4˚C or for 20 minutes at RT.
2. Addition of bFGF and Activin A may help cell culture results.

Neuro-Sure™ Neural Crest Stem Cell Culture Media

Cat #: ASM-4021
Product Size: 100 ml
Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems 1,2. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells3,4. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
Product description and usage: Add 2 mL of NCSC medium supplement to 498 mL of
NCSC culture medium to produce 500 mL of NCSC media.

Storage conditions for NCSC medium: -80˚C; 1 year, 4˚C; two weeks. Minimize exposure
to direct light.

Storage condition for NCSC supplement: -80˚C; 1 year, 4˚C; two weeks.

Storage condition for NCSC media: 4˚C; two weeks. Minimize exposure to direct light.

Shipping: On dry ice.

Recommended procedure:
1. Thaw NCSC medium either overnight at 4˚C or for several hours at RT.
2. Thaw the NCSC medium supplement at RT.
3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4˚C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 370C water bath.

ESC-Sure™ Serum-/Feeder- Free hESC/iPSC Culture Medium (SFFM)

Cat #: ASM-5010
Product Size: 100 ml
Our Serum, feeder-free medium formulations are optimized for human ESC and iPSC culture. It contains growth factors and extracellular matrix proteins secreted by MEF cells.

SFFM is ready-to-use.
- Only need to add FGF2
- Culture dish coating: matrigel or similar matrix.

Blastocyst-Sure™ KSOM Embryo Culture Medium, with Phenol Red (with Amino Acid)

Cat #: ASM-5023
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)

Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.

Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.

Blastocyst-Sure™ KSOM Embryo Culture Medium, without Phenol Red (with Amino Acid)

Cat #: ASM-5024
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)

Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.

Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.

Above: hESC in ESC-Sure
conditioned medium
Bottom: hESC on MEF TM

Background: Traditionally human embryonic stem cell (hESC) culture requires a mouse embryonic fibroblast (MEF) cells’ support, which makes the protocol complicated. Our Serum- and Feeder-free medium that is for human ESC culture contains all the growth factors needed in hESC culture, except the bFGF; eliminate the routine preparation of feeder. It is a ready-to-use product for a serum/feeder-free system. Our medium makes your cell culture more efficient and easier to control.
Serum- and Feeder-free medium (1x) for proliferation of pluripotent human embryonic stem cell (hESC) culture in a serum/feeder-free system. Each and every batch has been tested for hES/iPS cells pluripotency. All you need to add is 20 ng/ml bFGF.
Product description: 100 mL of hESC-Sure
Source: Chemical plus growth factors
Storage: -80°C
Shipping: Shipped on dry ice.
Recommended procedure:
Human ESC culture procedure using hESC-Sure Serum- and Feeder-free medium:
1. Coat culture dish with Matrigel the day before.
2. Wash Matrigel (BD Biosciences Cat# 354277) coat dishes with DMEM/F12 medium(Targatt Cat# ASM-5002).
3. Once human ES cell were confluent or cultured for more than 5 days, disaggregated cells
with 1mg/ml Dispase(Dilute 5mg/ml stock solution with DMEM/F12 media), incubate at 37
C for 3 minutes.
4. Wash container with 1x PBS, and to scrape colonies off the bottom of the container with cell
scraper.
5. Collect the cells in the 50ml or 15ml falcon tube, pellet by centrifugation at 700rpm for 5 min
at room temperature.
6. Resuspend hESC pellets in hESC-Sure Serum- and Feeder-free culture medium supplemented with 20 ng/ml bFGF.
TM
7. Split cells at 1:3 to 1:5 ratio every 4 to 5 days using 1mg/ml Dispase (diluted with
DMEM/F12).
8. 1x freezing medium contains 90% knockout serum and 10% DMSO.

 Price: 104 EUR