Researchers from Massachusetts General Hospital in Boston are getting closer to the moment will be using tissue engineering to create ear shells for patients who are born with malformed or have lost those parts of your body to incidents BBC .

In tissue engineering material is used by the patient and in the laboratory are "grown" organs to replace those missing in people for various reasons. Years ago, scientists were able to raise their ear about the size of a baby on the back of a mouse.

In yet another accomplishment that reported the journal Journal of the Royal Society Interface, talking about the following: scientists take living tissue from cows or sheep and then "grow" on a flexible matrix of titanium, reproduced on the basis of three-dimensional representation of a real human cochlea. Then again comes to the aid experimental mouse - rat immunosuppressed organ is implanted to reach the required size.

Dr. Thomas Cervantes, who heads the team, told the media that the first such human ear in real terms, it's a fact. After 12 weeks, the rat donor is willing to part with their ear and it should be provided to the patient for implantation.

Scientists in Japan cloned a mouse from a drop of blood. Moving blood cells taken from the tail of the mouse donor were used to create the clone, said researchers from the center Ricken Bioresources. Some time ago the same scientists have created nearly 600 exact genetic copies of a mouse.

Mice were cloned from different sources of donor cells, including white blood cells of the lymph nodes, bone marrow and liver. Japanese researchers studied whether the moving blood cells can be used for cloning. Their goal was to find an easy source of donor cells for cloning valuable scientifically types of laboratory mice.

Scientists led by Atsuo Ogura of Bioresources Rikes center in Tsukuba, blood taken from the tail of a mouse donor, isolated white blood cells and used kernel cloning experiments using the same technique as for the cloning of Dolly the sheep in Edinburgh.

The process, known as somatic cell nuclear transfer involving transfer of the nucleus from a cell in the adult body as blood cells or skin unfertilized egg, which was removed the nucleus. Scientists, whose research was published in the journal Biology of Reproduction, said that it shows for the first time that mice can be cloned using the nucleus of peripheral blood cells.

"These cells can be used to clone immediately after isolation, without requiring euthanasia of donor" complemented researchers.

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Studies of "drug" are still in a very early stage and tests are performed only on mice.

Soon fans will be able to cup sober just one pill. Researchers made ​​a cocktail of alcohol metabolizing enzymes rapidly reduce the level of alcohol in the blood while drunk mouse forward "Daily Mail". 

"Treatment", which compares with having millions of liver cells in your stomach, you may have a lasting impact on fans of spirits. Yangfeng Lou - professor of chemistry and biomolecular engineering, and Cheng Zhi - Professor of Biochemistry and Molecular Biology at the University of Southern California, injected mice with drunk nanocapsule containing two enzymes. One of the enzymes - oxidase comes as a by-product of hydrogen peroxide, which can be harmful. That's why he has to work with another enzyme to break down hydrogen peroxide. The study shows that drunken mice that were injected with these nanocapsule, sobered much faster than those who did not receive enzyme "treatment". The breakthrough is still in a very early stage and can not speak for its application in humans.

But expert Lou argues that experience can lead to the invention of a new drug to act as an antidote to alcohol. He states that "drug" can be taken as a simple pill. "It's like you have millions of liver cells in your stomach that help you revise alcohol," said Professor Lu.

We provide a high quality service for the generation of gene-targeted knock-in and knock-out mouse models. Our team has decades of experience in vector design, ES cell targeting and mouse handling. We will discuss your project needs with you and custom design your knockin/knockout mouse model. Our service includes consultation on available gene targeting vector design strategies and project assessment based on your model requirements.

Service Milestones for the Generation of Knock-in/Knock-out Mouse Models

• - Gene targeting vector construction and sequencing
• - ES cell targeting, screening and expansion of positive ES cell clones
• - Karyotyping of targeted ES cells
• - Cre/FLP recombination (optional)
• - Chimera production by blastocyst injection
• - Breeding of chimeras for germline transmission
• - Genotyping of offspring
• - Transfer of heterozygous targeted mice to the customer
• - Optional: breeding to generate homozygous mice

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Please contact us with any questions regarding services not listed. We are happy to discuss your project with you and design customized strategies. We also offer Transgenic Mouse (Random Insertion) and Transgenic Rat Services (knockin, knockout).

Testimony of Dr. Zhenheng Guo, PhD, Assistant Professor of Internal Medicine, Division of Endocrinology and Molecular Medicine, University of Kentucky

Project: Germline-transmitted, conditional knock-out mice by tetraploid complementation

"Overall, I am very satisfied with the quality of your service. After we received the mice, we did extensive studies to characterize them. All of the PCR reactions confirm the gene modifications are in the correct sites. We have also crossed mice with Cre mice to demonstrate that the Cre deleted the exon in heterozygous pups as expected. All data obtained showed that the mice were correctly targeted. In addition, I am very happy with the frequent communication with your scientists during the process. I highly recommend your services."

Project types

Knock-in Mouse Models

The introduction of a gene into a specified location of the mouse genome can be used for various applications. Mice can be homozygous or heterozygous for the inserted gene.

• - Reporter genes (e.g. GFP, lacZ) are used for expression analysis of a gene of interest. The reporter gene is inserted into the gene of interested in-frame (non-disruptive), allowing for visualization of temporal and spatial gene expression pattern.
• - Humanized disease models can be generated by inserting a human mutant gene or gene fragment into the corresponding mouse gene. The diseased human allel is thus transcribed in the appropriate genomic context and can be analyzed on behavioral, pathological, cellular and/or molecular level.
• - DNA recombinases (CRE, FLP) can be inserted into a gene of interest. The celltype-specific expression of the recombinase then allows for gene inactivation in the desired tissue after crossing this knock-in mouse with a conditional knockout mouse.

Knock-out Mouse Models

The knockout technology is most commonly used for gene inactivation, either in a constitutive or conditional fashion.

• - Constitutive knock-out mouse models are widely used to study gene function. The gene of interest is permanently inactivated in all cells of the animal. However, this non-conditional knock-out may cause lethality and is therefore not always recommended.
• - Conditional knock-out models are inducible - the targeted gene is excised after crossing the mouse with a Cre-transgenic mouse line. Gene inactivation can be celltype-specific and/or chemically induced.