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GmbH Marienbongard 20
52062 Aachen Deutschland
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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Kuiper 1
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Piazza Giacomo Matteotti, 6, 24122 Bergamo
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San Jose, CA 95123
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
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QuantiChrom™ Urea Assay Kit
Catalog number : DIUR-500
Quantity: 500 tests
Availability: Yes
DESCRIPTION:
Urea is primarily produced in the liver and secreted by the kidneys. Urea
is the major end product of protein catabolism in animals. It is the
primary vehicle for removal of toxic ammonia from the body. Urea
determination is very useful for the medical clinician to assess kidney
function of patients. In general, increased urea levels are associated with
nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal
diseases, e.g., congestive heart failure, liver diseases and diabetes.
Decreased levels indicate acute hepatic insufficiency or may result from
over-vigorous parenteral fluid therapy.
Simple, direct and automation-ready procedures for measuring urea
concentration or blood urea nitrogen BUN in biological samples are
becoming popular in Research and Drug Discovery. BioAssay Systems'
urea assay kit is designed to measure urea directly in biological samples
without any pretreatment. The improved Jung method utilizes a
chromogenic reagent that forms a colored complex specifically with urea.
The intensity of the color, measured at 520nm, is directly proportional to
the urea concentration in the sample. The optimized formulation
substantially reduces interference by substances in the raw samples.
KEY FEATURES:
Sensitive and accurate. Use 5 μL samples. Linear detection range 0.08
mg/dL (13 μM) to 100 mg/dL (17mM) urea in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single
working reagent and incubation for 20 min. Can be readily automated as a
high-throughput assay for thousands of samples per day.
Improved reagent stability and versatility. The optimized formulation has
greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Low interference in biological samples. No pretreatments are needed.
Assays can be directly performed on raw biological samples i.e., in the
presence of lipid and protein.
APPLICATIONS:
Direct Assays: urea in serum, plasma, urine, milk, cell/tissue culture,
bronchoalveolar lavage (BAL) etc.
Drug Discovery/Pharmacology: effects of drugs on urea metabolism.
Environment: urea determination in waste water, soil etc.
KIT CONTENTS: (500 tests in 96-well plates)
Reagent A: 50 mL Reagent B: 50 mL
Urea standard: 1 mL 50 mg/dL
Storage conditions. The kit is shipped at room temperature. Store all
components at 2-8°C. For long-term storage, keep standard at –20°C.
Shelf life: 12 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Price: 339 EUR
