Suppliers
Contact Us
 GENTAUR Europe BVBAVoortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, 
Macedonia,
Montenegro, 
Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
AccuPower 2X Greenstar master mix solution
Ready-to-use cocktail containing all components,except primer, for the amplification and detection of DNA in real-time quantitative PCR(qPCR).
The AccuPower® 2X Greenstar qPCR Master Mix is a ready-to-use cocktail containing all components,except primer, for the amplification and detection of DNA in real-time quantitative PCR(qPCR). It combines the automatic "Hotstart" technology of Top DNA polymerase and SYBR Green I fluorescent dye to deliver excellent sensitivity in the quantification of target sequences, with a linear dose response over a wide range of target concentration. Volumes are provided for 100 or 200 amplification reactions of 50ul each.
Features and Benefits
| High Specificity : | AccuPower® 2X Greenstar qPCR Master Mix provides more accurate Real-time PCR result by application of Hot-start method. |
| Stability: | The chemical stabilizer maintains enzyme activity for 2 years at -20°C |
| Simplicity: | Ready to use, AccuPower® 2X Greenstar qPCR Master Mix contains everything of Real-time PCR excluding primer and template. |
| Reproducibility: | Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment |
Applications
- Real-time quantification of DNA and cDNA targets
- Gene expression profiling
- Microbial & Viral pathogen detection
AccuPower DualStar qPCR PreMix
AccuPower DualStar qPCR PreMix is a lyophilized PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart qPCR master mix.
DualStar qPCR PreMix eliminates nonspecific reaction, while providing high sensitivity, due to our novel HotStart methodology. Gentaur uses a unique enzyme-mediated HotStart PCR that provides robust and reproducible results. Gentaur’s Top DNA Polymerase is inhibited at lower temperatures (< 70°C) by pyrophosphate. However, Top DNA Polymerase is rendered fully active at temperatures above 70°C via pyrophosphate hydrolysis with our thermostable pyrophosphatase. This prevents the formation of mis-primed products and primer-dimers during the reaction set up process resulting in improved PCR specificity. It is ideal for nucleic acid amplification reactions involving complex genomic or cDNA templates, very low copy targets, and multiplex reactions.
DualStar Plus qPCR PreMix goes a step further by providing resistance to common inhibitors of PCR (Blood-EDTA, Hemoglobin, Humic Acid from various sources, etc.) providing you with robust and reliable results, even with poor quality samples. Tested for resistance against many common inhibitors of PCR, DualStar plus is the enzyme of choice for any PCR that you want to work right – the first time and every time!
Features and Benefits
| Ease of use: | Just add template and primers and start your PCR. dNTPs, buffer and enzyme are provided. |
| Stability: | Stable at room temperature for a month and for 2 years in a -20°C freezer |
| HotStart: | Unique enzyme mediated HotStart results in greater specificity and more robust reactions |
| Reproducibility: | Each batch is produced under strict ISO quality controls. |
| DualStar Plus qPCR PreMix: | Available for less samples that will not work in other systems due to low purity |
Specifications
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
Terminal transferase activity: Yes
Fragment Size: 1 kb
Application
- Gene expression profiling
- Target DNA quantification
- Microbial detection
- SNP analysis
- Viral/bacterial pathogen load determination
- Evaluation of primer pair performance for probe-based real-time PCR
Figure 1. Real-Time PCR Data of AccuPower® DualStar™ qPCR PreMix
AccuPower® DualStar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (107 ~ 101copies/reaction).
A: Amplification curve. West Nile Virus (WNV) primers and TaqMan-based probe were added into DualStar qPCR Premix. A series of WNV positive control diluents were tested.
B: Standard curve. All data were obtained using Exicycler™ 96 Real-Time Quantitative PCR System (Gentaur).
Figure 2. Data using Various kinds of Real-time PCR Instruments
AccuPower® DualStar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (107 ~ 101copies/reaction).
A: Amplification curve. and standard curve using ABI 7500 Format Fast Real-time PCR machine (Applied Biosystems). A series of WNV positive control diluents were tested.
B: Amplification curve and standard curve using ABI 7500 Format Real-time PCR machine (Applied Biosystems).
C: Amplification curve and standard curve using Opticon Real-time PCR machine (MJ Research, currently Bio-Rad).
Figure 3. Comparison of detection sensitivity between AccuPower® DualStar™ qPCR PreMix and other supplier's master mixture
West Nile Virus (WNV) primers and TaqMan-based probe were added into AccuPower® DualStar™ qPCR Premix and other supplier's master mixture. A series of WNV positive control diluents were tested. Reaction mixtures were prepared and qPCR was performed according to each supplier's protocol. All data were obtained using ABI 7500 Format Fast Real-time PCR machine (Applied Biosystems).
