The AccuPower Taq PCR PreMix is a convenient lyophilized PCR master mix containing Taq DNA polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer and is aliquoted in 8-strip PCR tubes. 
The premix retains its activity for over a month at room temperature and is stable for two years in -20°C freezer. 
AccuPower Taq PCR PreMix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer.

Features and Benefits

Up to 10 per cent of the active genes of an organism that has survived 80 million years without sex are foreign, a new study from the University of Cambridge and Imperial College London reveals. The asexual organism, the bdelloid rotifer, has acquired a tenth of its active genes from bacteria and other simple organisms like fungi and algae.

The findings were reported Nov. 15 in the journal PLoS Genetics.

Bdelloid rotifers are best known for going 80 million years without sex, as they have evolved to reproduce successfully without males. Many asexual creatures go extinct without the benefit of traditional genetic evolution. However, bdelloids have flourished by developing ingenious ways of overcoming the limitations of being asexual.

Bdelloids have also developed the fascinating ability to withstand almost complete desiccation when the freshwater pools they typically live in dry up. They can survive in the dry state for many years only to revive with no ill effect once water becomes available again.

"We were thrilled when we discovered that nearly 10 per cent of bdelloids' active genes are foreign, adding to the weirdness of an already odd little creature," said Professor Alan Tunnacliffe, lead author of the study from the University of Cambridge. "We don't know how the gene transfer occurs, but it almost certainly involves ingesting DNA in organic debris, which their environments are full of. Bdelloids will eat anything smaller than their heads!"

Because some of the foreign genes are activated when the bdelloids begin to dry out, the researchers believe that the genes play a role in bdelloids' ability to survive desiccation.

Professor Tunnacliffe added: "Other researchers have shown that bdelloids contain powerful antioxidants, which help protect them from the toxic oxidising agents that are the by-products of desiccation. These antioxidants have not yet been identified, but we think that some of them result from foreign genes."

For the study, the researchers extracted all of the messenger RNA (genetic code similar to DNA which provides a blueprint for the creation of proteins) from bdelloid rotifers and sequenced each message, creating a library of the animal's active coding information. Using a supercomputer, they then compared these messages with all other known sequences and found that in many cases similar sequences had been found in other organisms.

Strangely, however, these other organisms were often not animals, but simple microbes. This means that bdelloids have genes that are not present in other animals, but have been acquired from micro-organisms and adapted for use in the rotifer.

The research was funded by the Biotechnology and the Biological Sciences Research Council (BBSRC) and the European Research Council.

Antibodies for :

Arabidopsis thaliana, Chlamydomonas sp. , diatoms, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pisum sativum, Populus sp., Spinacia oleracea, Synechococcus sp., Zea mays. And many more!

For: western blot, immunolocalizations, IP

Speed up Your research with the most comprehensive plant and algal antibody collection from original manufacturer

All antibodies are carefully validated by our own testing laboratory or in collaboration with scientists in the field. 

Contact us and we will help you to find the right antibody for your application!

Click for PDF document

The p40 antibody, catalog number A00112 is provided in concentrated form or in ready-to-use as a 2ml, 7ml, or 25ml.  This antibody is very important as it is helpful in the indication of several different carcinomas and has a wide-spread use in the market.  We have developed the p40 antibody as a replacement antibody for the p63 antibody.  The marker is highly specific for squamous basal cells and is more specific than the p63 marker.  One of the most important functions of this antibody is the ability to differentiate between adeno carcinoma and squamous cell carcinoma for lung cancer.  As you are aware the difference between those carcinomas will determine what avenue of treatment will be applied.  The p40 is specific for indication of the squamous cell carcinoma.  We will soon be releasing our Napsin A antibody for the adeno carcinoma.  These two antibodies work well in conjunction with one another and they will help the pathologist determine what method of treatment is necessary.

The p40 is also an excellent antibody in the use of prostate cancer.  In the early stages of prostate cancer the basal cells inside the ducts will be stained by the p40.  This will help indicate the prostatic intraepithelial neoplasia (PIN) that is considered to be a pre-malignancy as it is a carcinoma in situ of the prostatic glands.  As the cancer progresses the basal cells will be seen throughout the tissue as the cells become invasive.  This antibody can help determine the stage of carcinoma by indicating locations of the basal cells that are stained.

The p40 antibody is also being used with breast cancer as the antibody will stain myoepithelial cells along the ducts of the mammary glands.  This antibody is excellent in determining if Atypical ductal hyperplasia (ADH) is present which can indicate a risk factor for breast cancer and serves as early detection of breast cancer.  This marker will also indicate Ductal Carcinoma in Situ (DCIS) similar to the way it works on prostate cancer.  This is the most common non-invasive type of breast cancer.  The marker will indicate DCIS by highlighting that the cells are still within the ducts.  As breast cancer progresses and becomes invasive those ducts will breakdown and the myoepithelial cells will be seen throughout the tissue and become Invasive Ductal Carcinoma (IDC). 

p40 (p63 Delta); Polyclonal (Ready-To-Use)

Species: Rabbit
Clone: Polyclonal
Isotype: Rabbit IgG
Species Reactivity: Human.
Cellular Localization: Nuclear.
Specificity: The new marker p40 (p63 delta) is highly specific for squamous basal cells.

 Data sheet | MSDS

p40 (p63 Delta); Polyclonal (Concentrate)

Species: Rabbit
Clone: Polyclonal
Isotype: Rabbit IgG
Species Reactivity: Human.
Cellular Localization: Nuclear.
Specificity: The new marker p40 (p63 delta) is highly specific for squamous basal cells.

Data sheet | MSDS

Click to enlarge

More information about our new products

Prices

In his most recent study, Angelos Halaris, MD, PhD, and colleagues found that an inflammatory biomarker, interleukin-6, was significantly higher in the blood of 48 patients diagnosed with major depression than it was in 20 healthy controls. Interleukin-6 has been associated with cardiovascular disease. Halaris presented findings at a joint congress of the World Psychiatric Association and International Neuropsychiatric Association in Athens, Greece. At the congress, Halaris formally proposed creation of a new Psychocardiology subspecialty.

Forty to 60 percent of heart disease patients suffer clinical depression and 30 to 50 percent of patients who suffer clinical depression are at risk of developing cardiovascular disease, Halaris said.

Stress is the key to understanding the association between depression and heart disease. Stress can lead to depression, and depression, in turn, can become stressful.

The body’s immune system fights stress as it would fight a disease or infection. In response to stress, the immune system produces proteins called cytokines, including interleukin-6. Initially, this inflammatory response protects against stress. But over time, a chronic inflammatory response can lead to arteriosclerosis (hardening of the arteries) and cardiovascular disease.

It’s a vicious cycle: depression triggers a chronic inflammation, which leads to heart disease, which causes depression, which leads to more heart disease.

Clinical depression typically begins in young adults. “Treating depression expertly and vigorously in young age can help prevent cardiovascular disease later on,” Halaris said.

Physicians often work in isolation, with psychiatrists treating depression, and cardiologists treating cardiovascular disease. Halaris is proposing that psychiatrists and cardiologists work together in a multidisciplinary Psychocardiology subspecialty.

A Psychocardiology subspecialty would raise awareness among physicians and the public. It would forge closer working relationships between psychiatrists and cardiologists. It would formalize multidisciplinary teams with the requisite training and expertise to enable early detection of cardiovascular disease risk in psychiatric patients and psychiatric problems in heart disease patients. And it would provide continuing education to physicians in the safe and correct use of medications in cardiac patients who have psychiatric disorders.

“It is only through the cohesive interaction of such multidisciplinary teams that we can succeed in unravelling the complex relationships among mental stress, inflammation, immune responses and depression, cardiovascular disease and stroke,” Halaris said.

Halaris is Medical Director of Adult Psychiatry and a Professor in the Department of Psychiatry and Behavioral Neurosciences at Loyola University Chicago Stritch School of Medicine.

Check out our new Cardiovascular products

A drug currently approved to treat mouth ulcers has shown promise in animal studies for being a contender in pharmaceutical weight loss. Amlexanox was found by University of Michigan researchers to produce weight loss in obese mice without any change in diet or exercise habits.

For the study, mice fed a high calorie diet until they became obese were injected with amlexanox. The animals lost weight, despite consuming the same amount of calories. The researchers also noted a loss in overall body fat, a decrease in fatty liver, and a reversal of obesity-induced type 2 diabetes. Once taken off the amlexanox injections, however, the mice experienced weight gain.

Amlexanox may work by changing the action of genes that control metabolism versus working as an appetite suppressant.

When the drug was injected in mice, the drug worked by increasing metabolism, not by suppressing appetite.

"One of the reasons that diets are so ineffective in producing weight loss for some people is that their bodies adjust to the reduced calories by also reducing their metabolism, so that they are 'defending' their body weight," says Dr. Alan Saltiel, the lead researcher at the University of Michigan.

 "Amlexanox seems to tweak the metabolic response to excessive calorie storage in mice."

The findings were published Sunday in the journal Nature Medicine. Clinical trials are expected to begin later this year to test the drug's effectiveness in humans.

WSC-2610 MyMiniBLOCK

Do you need only heating function with block incubator? WSC-2610 MyMiniBLOCK is a microprocessor-controlled product using advanced thermoelectric technology. With this technique, more stable and accurate up to 100 C temperature control is achieved. In compact body, very various use such as sample preparation of electrophoresis, DNA/RNA denature and reaction, trans-information of E. coli, etc. can be applied.

WSC-2620 PowerBLOCK

WSC-2620 PowerBLOCK is able to reach set-temperature in mins.
It can reach to the target temperature in about 7min, you can easily use it with different temperature just after using with previously set temperature. Wide and stably accurate temperature setting is from –10 to 100o C. It can be used for very wide applications and used in different temperature settings continuously.

WSC-2630 PowerBLOCK Shaker

Characteristics
· Detection kit for Animal Species
· Real-time PCR based on TaqMan™ probe-based technology
· Rapid & simple reaction
· No contamination : closed system
· No error : Internal positive control
· Easy to operation in less than 2.5 hrs

Kit Components
· Primer/probe mixture
· 2X Real-time PCR Master Mix
· Control

Protocol
After DNA Ext.
· Step 1 : Mix extracted DNA with the Kit’s reagents
· Step 2 : Real-time PCR run
· Step 3 : Analysis

Exp

 Products:

Quantity: 4800 (in case - Racked - 96)
Availability: Yes
Details:
Barrier Tips to Give You Complete Peace of Mind
Protect your work from contamination with one of the industry’s most efficient aerosol filter assemblies. Our proven range of barrier tips reliably prevent aerosol transfer to give you complete peace of mind when performing your most sensitive assays.

BT10XL Series - 10 µl Extended Length Barrier Tip
GENTAUR offers a complete selection of high quality pipet tips for your entire laboratory's needs.

Specifications:
* GLP Compliance Certified
* S³ Low retention Polymer Technology
* No detectable nucleic acid
* No detectable endotoxin
* No detectable endonucleases
* No detectable PCR inhibitors
* Pre-sterilized
* NEPTUNE S³ Tip without any residue
* Non S³ Tip with sample residue
S3 Low Retention Polymer Technology for Total Sample Recovery.
Exclusively form CLP!
The trend in research toward ever smaller reaction volumes has many benefits, including improved reaction kinetics, more consistent results and lower overall costs. However, low volume techniques require dispensing very small volumes of reagents with high degree of accuracy and consistency
Pipet tips produced from standard polymers will variably retain biological solutions, preventing accurate and repeatable results. Diamond polishing of the mold reduces the number of imperfections producing a smoother surface. Silicone treatment of tips further reduces retention, but can leach out and interfere with reactions, or degrade at autoclaving temperatures.
CLP was the first company to address this challenge and develop a novel polymer technology that produces a Super Slick Surface S3 on plastics. Traditionally, small occlusions and cavities within the molded plastic resulted in significant sample loss. Our third generation S3 polymer system results in a microscopically uniform surface, which virtually eliminates sample hold-up providing the most accurate sample delivery possible. CLP’s S3 polymer combined with our advanced production process produces the most consistent low retention plastics in the industry.

Prices:

1 box 10x96 Tips for 59 EUR
10boxes 10x96 Tips + 1 labpet of choice for 480 EUR
20boxes 10x96 Tips+ 3 labpets for 880 EUR
30boxes 10x96Tips + 5 labpets for 1290 EUR

*1250ul are 8x96 in a box

Click here for more Barrier Tips products