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Howard Frank Turnberry House
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Whetstone London N20 9BH
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San Jose, CA 95123
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Avian influenza virus (H7N9) Real Time RT-PCR Kit
Catalog number: RR-0309-02
* Will be delivered in 1-2 weeks
 |
Products related to Avian influenza virus (H7N9) Real Time RT-PCR Kit
1. Intended Use
Avian influenza virus H7N9 real time RT-PCR kit is used for the detection of gene H7 and gene N9 of avian influenza A subtype H7N9 in human nasal and pharyngeal secretions and bird fece by using real time PCR systems.
2. Principle of Real-Time PCR
The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the
fluorescence intensities during Real Time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.
3. Product Description
Highly pathogenic avian influenza (HPAI) caused by certain subtypes of influenza A virus in animal populations, particularly chickens, poses a continuing global human public health risk. Direct human infection by an avian influenza A (H5N1) virus was first recognized during the 1997 outbreak in Hong Kong. The avian influenza virus H7N9 is one subgroup among the larger group of H7 viruses. Some cases of human infection with H7N9 virus in China are confirmed till early April of 2013. Avian influenza virus H7N9 real time RT-PCR kit contains a specific ready-to-use system for the detection of avian influenza virus H7N9 by Reverse Transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The master contains Super Mix for the specific amplification of the avian influenza virus H7N9 RNA. The reaction is done in one step real time RT-PCR.
The first step is a reverse transcription (RT), during which the avian influenza virus H9 RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by polymerase chain reaction. Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The detection of amplified avian influenza virus H7N9 DNA fragment is performed in fluorimeter channel FAM and HEX/VIC/JOE with the fluorescent quencher BHQ1. In addition, the kit contains a system to identify possible PCR inhibition by measuring the FAM fluorescence of the internal control (IC).
