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Monday, 25 November 2013 16:25

Uracil DNA-Glycosilase (UDGase)

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Cat. num.: BIO-27044

Quantity: 500 Units

Description
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM).
UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.

Source: Recombinant E. coli strain carrying the over-expressed modified gene of Uracil DNA Glycosylase from E. coli.

Features
• Removal of uracil from uracil-containing DNA
• Novel temperature sensitive mutant irreversibly inactive after
heating

Applications
• UDG treatment of uracil-containing DNA to prevent
amplification by DNA Polymerases
• Used to investigate features of protein-DNA interactions

Storage and stability:
Uracil DNA Glycosylase (UDG) is shipped on Dry/Blue Ice. It should be stored at -20°C upon receipt. When stored under optimum conditions, the reagents are stable for 24 months from date of purchase.

Storage and Dilution Buffer:
20mM Tris-Cl, pH 8.0, 0.1M NaCl, 0.1mM EDTA, 1mM DTT, 0.1% stabilizer and 50% glycerol.